Intestinal stem cells (ISCs) play a critical role in gut repair, aging support, and the maintenance of homeostasis, owing to their proliferative capacity and self-renewal abilities (de Morree and Rando, 2023; Ermolaeva et al., 2018). However, their functionality declines significantly with age, resulting in reduced tissue regeneration and a higher risk of age-related diseases (Brunet et al., 2023; Ermolaeva et al., 2018; Lopez-Otin et al., 2023). Addressing this decline in ISC performance during aging presents a substantial challenge. Drosophila ISCs are an ideal model for studying development and aging because of their genetic richness, ease of manipulation, and similarity to mammalian tissues (Jasper, 2020). As the primary mitotically active cells in the Drosophila gut, ISCs show considerable flexibility in response to dietary and stress signals. They can divide symmetrically to produce more ISCs or asymmetrically to generate progenitor cells, such as EnteroBlasts (EBs), EnteroEndocrine cells (EEs), or large polyploid EnteroCytes (ECs) (Guo and Ohlstein, 2015). Although manipulating signaling pathways or dietary restrictions has shown promise in regulating ISC functions and extending lifespan in animal models, these approaches face practical limitations for broader application. Additionally, the specific impact of nutrients or dietary elements on ISC adaptive resizing is still not well understood (Du et al., 2020; Fan et al., 2023; Fang et al., 2019; Kim et al., 2022; Qin et al., 2024; Yan et al., 2022).

Kaempferol, a naturally occurring flavonoid prevalent in fruits and vegetables, emerges as a strong candidate for dietary interventions (Gidaro et al., 2016). Its widespread dietary availability, minimal toxicity, cost-efficiency, and ease of daily incorporation make it well-suited for extensive use (Gidaro et al., 2016; Yang et al., 2023). Numerous studies have demonstrated kaempferol’s wide-ranging pharmacological benefits, including anticancer (Lee and Kim, 2016; Luo et al., 2012; Luo et al., 2011; Shrestha et al., 2021), anti-inflammatory (Wang et al., 2020), anti-obesity (Han et al., 2021), antiviral (Bangar et al., 2023; Gao et al., 2023), antioxidant (Guo et al., 2015; Wang et al., 2018), immune-modulatory (Bangar et al., 2023), and neuroprotective effects (Dong et al., 2023; Holland et al., 2020; Yuan et al., 2021). However, the specific effects of kaempferol on ISC aging and injury, along with its underlying mechanisms, remain inadequately explored. During the processes of aging and injury, reactive oxygen species (ROS) and endoplasmic reticulum (ER) stress can adversely affect ISC function and adaptability (Du et al., 2021; Yan et al., 2022). Given kaempferol’s capacity to neutralize ROS, reduce inflammation, and provide anticancer benefits (Guo et al., 2015; Han et al., 2021; Lee and Kim, 2016; Luo et al., 2012; Luo et al., 2011; Wang et al., 2018; Wang et al., 2020). We aim to investigate its potential to slow ISC aging and enhance damage recovery in Drosophila.

In this research, we demonstrate that Kaempferol serves as a potent natural compound by effectively curbing ISC hyperproliferation during aging. Notably, Kaempferol extends the lifespan of aged Drosophila by regulating gut function and sustaining homeostasis. Mechanistically, it mitigates ISC hyperproliferation by reducing ER stress through the upregulation of UPRER-related genes. Additionally, Kaempferol controls the excessive accumulation of ROS via ER-stress signaling and concurrently suppresses the insulin signaling pathway, contributing to delayed aging. Overall, we have identified Kaempferol as a novel natural phenolic compound that slows ISC aging and offers protective effects against damage.

Recent research has identified certain molecules with anti-aging properties in Drosophila, which have also shown similar benefits in mammals (Du et al., 2020; Fang et al., 2019; Wu et al., 2022). Building on these insights, we aimed to identify compounds in vegetables and fruits that could potentially extend lifespan. Several vegetable and fruit extracts have been reported to have such effects on Drosophila. Among these, Kaempferol, a flavonoid-rich in vegetables and fruits, drew our attention due to its well-documented health benefits and disease-preventing capabilities (An and Kim, 2015; Bangar et al., 2023; Dong et al., 2023; Gao et al., 2023; Gidaro et al., 2016; Guo et al., 2015; Han et al., 2021; Holland et al., 2020; Lee and Kim, 2016; Luo et al., 2012; Luo et al., 2011; Shrestha et al., 2021). To evaluate Kaempferol’s potential to inhibit ISC aging, we employed the esg-GFP/CyO reporter line, where green fluorescent protein (GFP) is regulated by the escargot (esg) gene (Korzelius et al., 2014), to assess its impact on ISC proliferation.

Previous research has indicated that in young Drosophila, ISCs either self-renew or differentiate into ECs and EEs (Guo and Ohlstein, 2015) (Figure 1A). With aging, These cells undergo aberrant proliferation and differentiation, leading to the excessive proliferation of ISCs and progenitor cells (Jasper, 2020). To examine this, we first fed Drosophila normal food for 26 days, then supplemented their diet with different concentrations of Kaempferol (0.2, 1.5, 20, 50 μM) for 14 days (Figures 1B, C). We assessed the number of ISCs and progenitor cells marked by esg-GFP+, pH3+ (a proliferation marker), and Dl+ (an ISC marker). Flies treated with 20 μM Kaempferol had fewer esg-GFP+, pH3+ (Figures 1C–E), and Dl+ cells (Figures 1F, G) compared to those fed diets added DMSO. At 50 μM, Kaempferol’s ability to reduce age-related ISC hyperproliferation diminished, suggesting potential side effects at higher concentrations (Figures 1C–E). TUNEL staining confirmed that the reduced ISC proliferation was not due to apoptosis of esg-GFP+ cells (Figure 1H). We also examined the impact of Kaempferol on ECs (marked with pros+) and EEs (marked with NRE+), which showed similar effects in aged flies (Supplementary Figures S1A–D). Overall, our data indicate that Kaempferol exerts a potent inhibitory effect on ISC hyperproliferation and attenuates gut hyperplasia in aged Drosophila.

Figure 1. Kaempferol Modulates the hyperproliferation of ISCs in a concentration-dependent manner during aging. (A) ISC division and differentiation model: ISCs (marked as Dl+ and esg+) undergo symmetric division for self-renewal and asymmetric division to produce enteroendocrine progenitor cells pre-EEs (marked as esg+ and Pros+) or EBs (marked as esg+ and NRE+). Pre-EEs differentiate into EEs (marked as Pros+), while EBs develop into ECs (marked as Pdm1+). (B) Model of Kaempferol feeding in aging Drosophila: Mock refers to flies hatched for 10 days and fed a standard diet. Kae indicates flies given Kaempferol. (C) Model of Kaempferol feeding in aging Drosophila: “Mock” denotes flies hatched for 10 days and fed a standard diet. “Kae” refers to flies treated with Kaempferol. (D) The proportion of esg-GFP+ cells to DAPI-stained cells per region of interest (ROI) in Drosophila midguts treated with varying concentrations of Kaempferol (0.2, 1, 5, 20, and 50 µM) compared to untreated controls. (E) The number of pH3+ cells per gut in 40-day-old Drosophila midguts, treated with varying concentrations of Kaempferol (0.2, 1, 5, 20, and 50 µM) or without treatment. (F) Representative images of immunofluorescence staining showing ISCs in Drosophila midguts, treated and untreated with 20 µM Kaempferol. Nuclei stained with DAPI (blue) Dl (red) marked ISCs. The top images represent the merged images and the bottom images represent ISCs. (G) The proportion of Dl+ cells to DAPI+ cells per ROI without or with 20 µM Kaempferol treatment. (H) Representative immunofluorescence images of ISCs in Drosophila midguts, with or without 20 µM Kaempferol treatment. Nuclei are stained with DAPI (blue), and ISCs are labeled with Dl (red). The top images show merged images; the bottom images highlight ISCs. Scale bars denote 25 μm (C, F, H). Error bars show SD. Statistical significance was assessed with Student’s t-tests: *p < 0.05, **p < 0.01, ***p < 0.001; ns indicates p > 0.05.

Previous studies have indicated that Drosophila midguts undergo continuous turnover and can regenerate after tissue damage (Lucchetta and Ohlstein, 2012). Chemical damage, such as from bleomycin (BLM), triggers ISC proliferation. Given Kaempferol’s positive effects on Drosophila gut health (Amcheslavsky et al., 2009), we examined whether it could enhance the gut’s resistance to injury. After a 1-day BLM treatment, the flies were placed on normal food for a 2-day recovery (Figure 2A). Surprisingly, 2 days post-injury, Drosophila treated with Kaempferol showed a more significant reduction in ISC numbers (marked by esg and Dl) and pH3+ cells compared to controls, indicating that Kaempferol supports intestinal repair and limits ISC hyperproliferation under stress (Figures 2B–E). Additionally, Paraquat (PQ), known to induce oxidative stress by increasing ROS production, is commonly used to cause intestinal damage in Drosophila (Szafrańska et al., 2016). Kaempferol notably extended the lifespan of both female and male Drosophila exposed to PQ/BLM treatment (Figures 2F, G; Supplementary Figures S2A, B). In conclusion, these findings imply that Kaempferol safeguards ISCs from damage while improving Drosophila’s resilience to stress.

Figure 2. Kaempferol promotes injury repair in the intestinal and enhances healthspan under injury conditions. (A) Model of Kaempferol feeding in Drosophila during BLM treatment. (B) Immunofluorescence images of Drosophila midguts fed with 25 μg/mL BLM, with or without the addition of 20 µM Kaempferol. Nuclei are stained with DAPI (blue), GFP highlights ISCs and progenitor cells (green), and Dl identifies ISCs (red). The top images show merged images, the middle images highlight ISCs and progenitor cells, and the bottom images focus on ISCs. (C) The proportion of esg-GFP+ cells to DAPI+ cells per ROI in midguts treated with BLM, with or without Kaempferol supplementation. (D) The proportion of DI+ cells to DAPI+ cells per ROI in midguts treated with BLM, with or without Kaempferol supplementation. (E) The number of pH3+ cells per fly gut, with or without 20 µM Kaempferol, under PQ/BLM treatment conditions. (F, G) Survival rates of female wild-type flies treated with DMSO (blue curve) or Kaempferol (red curve) under 20 mM PQ (F) or 5 μg/mL BLM (G) conditions. Data from three independent experiments are shown.

Ensuring the intestinal barrier remains intact is essential for upholding epithelial balance, shielding against pathogens, and supporting immune tolerance to beneficial bacteria (Gervais and Bardin, 2017; Naszai et al., 2015). The decline in ISC functionality and genetic integrity is a key factor in the deterioration of tissue function with aging (Biteau et al., 2008; Gervais and Bardin, 2017; Naszai et al., 2015). Since Kaempferol has shown promise in preventing age-related and injury-induced ISC decline, we further examined its potential to protect intestinal functions in Drosophila during aging. To assess this, we analyzed the copper cell region (CCR) condition in both young and old Drosophila. The CCR’s function decreases with age, disrupting intestinal acid-base balance (Li et al., 2016). In the Drosophila midgut, the CCR secretes acid, which is detected using bromophenol blue, a pH indicator. When intestinal homeostasis deteriorates and acid secretion decreases, the CCR remains blue. In contrast, if acid secretion improves and homeostasis is restored, the CCR turns yellow. Our results indicate that Kaempferol supplementation effectively maintains food intake and intestinal acid-base balance in aged Drosophila (Figures 3A–C). Additionally, Kaempferol improves intestinal function and enhances excretion in these flies (Figures 3D, E). Considering previous research and our findings that highlight the importance of intestinal regeneration for lifespan extension (Biteau et al., 2010), we also investigated Kaempferol’s impact on lifespan. Our data show that Kaempferol feeding extends lifespan in both male and female Drosophila (Figures 3F, G). In summary, Kaempferol supplementation counteracts the decline in intestinal function associated with aging and enhances the lifespan of Drosophila.

Figure 3. Kaempferol maintains gut homeostasis and extends the lifespan of Drosophila. (A) Images of intestinal acid-base regulation and non-feeding intestines. The black box highlights the CCR. “Non-eating” indicates wild-type flies that did not consume food, with the CCR shown in white. “Eating” refers to flies consuming bromophenol blue. “Homeostasis” shows the CCR in yellow, while “Perturbed” depicts it in blue. (B) The percentage of eating intestines in this experiment (A). Data from three independent experiments are shown. (C) The percentage of homeostasis intestines in this experiment (A). Data from three independent experiments are shown. (D) Representative images of waste deposits in flies treated with or without Kaempferol. Three separate experiments were conducted. (E) Deposits per field in experiment (D). Results are obtained from three independent experiment data. (F, G) Survival rates of female and male wild-type flies with DMSO (blue line) or Kaempferol (red line) treatment starting at 10 days old. Results are based on three independent trials.

The proliferation of ISCs is tightly controlled by gene expression (Guo and Ohlstein, 2015). To understand how Kaempferol influences ISC proliferation, we conducted the RNA-seq on midguts from flies treated with Kaempferol compared to controls. Principal component analysis (PCA) highlighted significant differences between the two groups (Figure 4A). Gene Ontology (GO) enrichment analysis indicated a dramatic increase in genes associated with the unfolded protein response (UPR) in flies treated with Kaempferol (Figure 4B). The UPRER refers to a subset of UPR genes activated by ER stress, which occurs when the ER becomes overwhelmed with misfolded proteins (Rath et al., 2012). To suppress ER stress, the UPR triggers its target genes to alleviate the accumulation of unfolded proteins by enhancing the production of several stress-responsive chaperones (Du et al., 2021; Hetz, 2012). GO enrichment analysis revealed significant differences in UPRER expression between Kaempferol-treated and control flies. We analyzed URPER-related genes and created a volcano plot to highlight the significance and fold changes of differentially expressed genes. The results showed that UPRER genes, such as Hsp23 and HSP68, were upregulated in the midguts of 40-day-old wild-type flies treated with Kaempferol compared to controls (Figure 4C). Heatmap analysis further identified UPR target genes (Hsp70Bbb, Hsp70Ab, HSC70-5, Hsp70Bb, Hsp68, Hsp70Bc, Hsp23, and Hsp70Aa) with markedly higher expression levels in the aged midguts of Drosophila treated with Kaempferol compared to untreated flies (Figure 4D). The UPRER is known to be crucial in regulating ISC hyperproliferation associated with aging (Du et al., 2021). Drawing on prior research and our current data, we propose that Kaempferol addresses aging-related gut hyperplasia through pathways involving UPRER genes. Moreover, we analyzed the expression levels of p-eIF2α, an essential transcription factor in the ATF4 ER stress pathway (Mao et al., 2019). Phosphorylated eIF2α (p-eIF2α) serves as a reliable biomarker for evaluating ER stress levels in cells (Rath et al., 2012), In Drosophila ISCs, p-eIF2α was significantly reduced (Figure 4E). This finding suggests that Kaempferol likely mitigates ISC aging by alleviating ER stress, as evidenced by the increased expression of UPRER target genes in aged flies treated with Kaempferol. Immunostaining further demonstrated that Kaempferol effectively lowered elevated p-eIF2α levels in these cells (Figure 4E). To validate Kaempferol’s effect on ER-stress signaling, we conducted RT-qPCR analyses, which revealed decreased levels of ER-stress-related genes (Ko and Brandizzi, 2024; Kumar and Maity, 2021). ER-stress-related genes (ATF6, ATF4, and XBP1) were reduced in the guts of aged Drosophila treated with Kaempferol (Figure 4F). These results suggest that Kaempferol may affect ISC proliferation by modulating ER-stress signaling.

Figure 4. Kaempferol inhibits ISC hyperproliferation through the ER-stress signaling. (A) PCA of midguts from 40-day-old wild-type flies: one cohort was treated with 20 µM Kaempferol, and the other with DMSO only. (B) GO pathway enrichment analysis was performed on upregulated and downregulated genes in a pair-wise comparison between 40-day-old Drosophila midguts treated with or without Kaempferol. (C) The volcano plot displays genes that are differentially expressed in 40-day-old Drosophila midguts with Kaempferol treatment compared to those without. Red dots highlight genes that are remarkably upregulated, blue dots mark genes that are remarkably downregulated, and gray dots indicate genes with no significant change. (D) The differential gene expression of UPRER in midguts of flies with Kaempferol feeding compared to the control group is shown in the heatmap. (E) Representative immunofluorescence images of p-eIF2α staining in midguts with or without Kaempferol treatment. The top images show merged images, while the bottom images focus on p-eIF2α signals. Three independent experiments were conducted. (F) RT-qPCR was used to measure the mRNA levels of ER-stress-related genes in the midguts of 40-day-old wild-type Drosophila, comparing those with and without Kaempferol treatment (administered starting at 26 days). Three independent experiments were conducted.

To further understand Kaempferol’s impact on aging ISCs, we reviewed existing studies, revealing that Kaempferol can inhibit antioxidant activity (Wang et al., 2018; Wang et al., 2020). It also impacts the expression of genes involved in ER-stress signaling (Figures 4B–D, F). ER stress is closely linked to ROS accumulation (Uddin et al., 2021). ER stress can lead to ROS production through mitochondrial DNA damage. Connecting ROS to mitochondrial stress as a consequence of ER signaling (Salminen and Kaarniranta, 2010). To investigate whether Kaempferol’s antioxidant effects are preserved in Drosophila, we measured the expression of antioxidant-related genes via RT-qPCR. Results showed increased levels of Catalase (Cat), Sod1, and Sod2 in aged esg+ cells treated with Kaempferol (Figure 5A). Additionally, Using the fluorescent probe DHE, we evaluated ROS levels in esg+ cells. The results indicated a dramatic decrease in ROS in aged flies that received Kaempferol (Figures 5B, C). To examine Kaempferol’s role in ISC homeostasis, we overexpressed Cat and used Keap1 RNAi in esg+ cells with a temperature-sensitive system (esgts), as Cat and Keap1 are key regulators of intracellular redox balance (Hochmuth et al., 2011). This approach revealed that Cat overexpression and Keap1 RNAi reduced ROS levels and ISC hyperproliferation, as shown by fewer esg+ and pH3+ cells in 40-day-old Drosophila (Figures 5D–F). Importantly, Kaempferol also decreased ISC proliferation in Drosophila with Cat overexpression and Keap1 depletion (Figures 5D–F), suggesting its role extends beyond antioxidant activity to regulating ISC homeostasis.

Figure 5. Kaempferol prevents ISC hyperproliferation through its antioxidative functions during aging. (A) RT-qPCR utilized to measure the mRNA levels of ROS-related genes (sod1, sod2 and Cat) in the midguts of 40-day-old wild-type Drosophila, comparing those with and without Kaempferol treatment (administered starting at 26 days). Three independent experiments were completed. (B) Representative images of DHE staining are provided. esg-GFP+ cells (green) and DHE staining (red) are highlighted by white dashed lines. The top images show merged images, while the bottom images focus on DHE staining. DHE fluorescence intensity was measured in esg-GFP+ cells, with each dot representing a single cell. (C) Quantitation of DHE fluorescence intensity in esg-GFP+ cells from this experiment. (D) Kaempferol further inhibited ISC proliferation in Uas-LacZ, Cat-overexpressed, and Keap1-depleted aged ISCs (starting from day 26). The top images show merged images, and the bottom images highlight esg+ cells, labeled with GFP. (E) The proportion of esg-GFP+ cells to DAPI+ cells per ROI in experiment (C). (F) The number of pH3+ cells per gut in experiment (C).

Kaempferol’s ability to further inhibit ISC proliferation in Drosophila with Keap1 RNAi and Cat overexpression suggests it might engage other pathways in its mechanism. Although lifespan varies greatly among species, fundamental genetic pathways that control longevity are conserved (Green et al., 2022; Guo et al., 2016; Hartl, 2016; Jasper, 2020; Melzer et al., 2020). A well-known example is the insulin/IGF-1 signaling (IIS) pathway (Giannakou and Partridge, 2007; Partridge, 2001). RNA-seq data show that Kaempferol significantly affects insulin signaling (Figure 4B). This pathway contains the insulin/IGF-1 receptor tyrosine kinase, and the serine/threonine kinase AKT (Giannakou and Partridge, 2007; Serrano et al., 2009). To investigate the impact of Kaempferol on this pathway, we measured insulin signaling activity by assessing levels of phosphorylated Akt (pAKT). Immunostaining results indicated that Kaempferol decreased pAKT levels in ISCs of aged flies (Figures 6A, B). Further validation was performed by inhibiting the insulin pathway using either InR RNAi (insulin-like receptor) or a dominant-negative (DN) form of InR, leading to a reduction in esg-GFP+ and pH3+ cells compared to controls (Figures 6C–E). Kaempferol supplementation also reduced these cell populations in aged flies, likely due to its antioxidant properties related to ER stress. These discoveries indicated that Kaempferol supports ISC homeostasis in aging Drosophila by partially inhibiting the insulin signaling pathway.

Figure 6. Kaempferol inhibits the hyperproliferation of ISCs in aging via the insulin signaling pathway. (A) p-AKT staining (red) in aged esg-GFP+ cells of Drosophila midguts, visualized through immunofluorescence imaging, comparing samples with and without Kaempferol treatment. The top images show merged images and the bottom images display p-AKT signals. (B) Quantitation of p-AKT fluorescence intensity in esg-GFP+ cells. Each dot indicates one esg-GFP+ cell. (C) Immunofluorescence images of ISCs in UAS-LacZ, UAS-InRDN, and InR RNAi Drosophila midguts, with or without Kaempferol treatment (starting from day 26). The top images show merged images, while the bottom images display esg+ cells. (D) The proportion of esg-GFP+ cells to DAPI+ cells per ROI in experiment (C). (E) The number of pH3+ cells per fly gut.

As mentioned, ER stress produces ROS in mitochondria through mitochondrial DNA damage (Forgie et al., 2022). This process highlights the link between ER stress and mitochondrial stress. To explore how Kaempferol influences proliferation via ROS and insulin signaling, we conducted experiments involving Keap1 RNAi or Cat overexpression, combined with the expression of a DN form of InR to suppress the insulin signaling pathway in 40-day-old ISCs. The findings indicated a notable reduction in both esg-GFP+ and pH3+ cells in these flies when compared to the control group (Figures 7A–C). Interestingly, Kaempferol did not increase these effects any further (Figures 7A–C). These results indicated that Kaempferol suppresses age-related hyperproliferation ISC by modulating both the insulin and ROS signaling pathways (Figure 7D).

Figure 7. Kaempferol regulates ROS and insulin signaling pathways to inhibit the hyperproliferation of aging ISC. (A) Immunofluorescence images of ISCs in UAS-LacZ, UAS-Cat overexpressed, UAS-InRDN, and Keap1RNAi with UAS-InRDN Drosophila midguts, with or without Kaempferol treatment (administered from day 26). The top images display merged images, and the bottom images show esg+ cells. (B) The proportion of esg-GFP+ cells to DAPI+ cells per ROI in experiment (A). (C) The number of pH3+ cells per fly gut (A). (D) Model of Kaempferol suppresses ISC hyperproliferation via insulin signaling pathway conjunction with ER-stress and ROS.

In recent years, researchers in injury repair and aging have concentrated on understanding the underlying mechanisms and devising advanced treatments to improve tissue repair and combat age-related diseases. A key area of interest has been the effect of natural substances on tissue damage and aging. Kaempferol, a compound found in various fruits, vegetables, and dietary supplements, has garnered attention for its potential health benefits (Bangar et al., 2023). Its neuroprotective, antioxidant, anti-inflammatory, anti-cancer, and immune-modulatory properties have been documented in both in vitro and in vivo studies, showing promise against conditions like stroke, Alzheimer’s, and cancer (Dong et al., 2023; Gao et al., 2023; Gidaro et al., 2016; Guo et al., 2015; Han et al., 2021; Lee and Kim, 2016; Luo et al., 2012; Luo et al., 2011; Wang et al., 2018; Wang et al., 2020). Our research further emphasizes Kaempferol’s role in repairing ISC damage and reducing ISC hyperproliferation related to aging. We found that Kaempferol effectively mitigates ISC dysfunction, prevents gut hyperplasia, preserves intestinal PH balance, and enhances food consumption and excretion in aged Drosophila. Additionally, Kaempferol supplementation significantly improved ISC recovery from injury and increased survival rates of Drosophila under stress.

Our investigation into the mechanisms of Kaempferol revealed its ability to mitigate ER stress, regulate ROS levels in ISCs, and influence insulin signaling. The ER plays a vital role in eukaryotic cells, managing protein synthesis, folding, and transport. Disruptions to ER function can lead to an accumulation of unfolded or misfolded proteins, which causes ER stress and activates the UPR to reestablish cellular equilibrium (Bhattarai et al., 2020). Aging affects ER chaperones and folding enzymes, impairing proteostasis and causing an accumulation of misfolded proteins. Ineffective UPR activation worsens ER stress by hindering the removal of these proteins (Uddin et al., 2021). Moreover, aging is associated with increased ER stress and elevated ROS levels (Salminen and Kaarniranta, 2010). ROS, reactive molecules derived from oxygen, serve as signaling agents in normal physiological processes but can cause oxidative stress when present in excess, contributing to aging and associated diseases (Yan et al., 2022).

Kaempferol affects ISC aging by modulating the IIS pathway, which is conserved from Drosophila to mammals and regulates numerous physiological functions, including growth, metabolism, reproduction, stress responses, and aging (Giannakou and Partridge, 2007). The Drosophila genome includes eight insulin/IGF-like peptide paralogs (Post et al., 2019). Studies show that decreased IIS can extend lifespan across various species, such as worms, Drosophila, mice, and humans. Meanwhile, increased IIS is associated with a shorter lifespan and a higher risk of age-related diseases (Giannakou and Partridge, 2007; Post et al., 2019). Moreover, the IIS pathway is vital for stem cell homeostasis, with Drosophila insulin-like peptides, which act through insulin receptors, being crucial for the regulation of stem cell proliferation (Li and Geng, 2010).

Aging leads to defects in ISCs across various tissues, impairing their ability to repair damage and maintain homeostasis (Brunet et al., 2023; Ermolaeva et al., 2018). Our study reveals that Kaempferol inhibits excessive ISC proliferation by modulating ROS and insulin signaling pathways during aging. However, the specific molecular mechanisms through which Kaempferol influences these pathways in aging ISCs and tissue repair require further investigation. It is possible that Kaempferol directly interacts with certain targets to regulate these pathways. Additional research is needed to clarify Kaempferol’s precise role in ISC function and its potential effects on ISC differentiation in the context of aging.

All Drosophila genotypes and their sources used in this study are listed in Supplementary Table S4.

Total RNA was extracted from 30 midguts dissected from wild-type female Drosophila for RNA sequencing. The samples were immediately frozen in liquid nitrogen and processed by Shenzhen Chengqi Biotechnology Co., LTD (China). Sequencing was performed on the Illumina NovaSeq 6000 platform (San Diego, US) with 150 bp paired-end reads, yielding over 20 million reads per sample. Initial quality checks of the raw FASTQ files were done using FastQC (v0.11.9, http://www.bioinformatics.babraham.ac.uk/projects/fastqc/). The processed reads were aligned to the Drosophila reference genome (Ensembl build BDGP6, https://support.illumina.com/sequencing/sequencing_software/igenome.html), Gene symbols were annotated based on the Drosophila BDGP6 genome from Ensembl. Differential gene expression was assessed using DESeq2 (v1.26.0) with default parameters, focusing on genes with an absolute log2 fold change exceeding 0.5 between modified (e.g., RNAi) and control samples, and a p-value of less than 0.05. Pathway analysis was performed with a cluster Profiler.

One hundred dissected midguts from 100 wild-type female Drosophila were placed in 4°C DEPC-PBS. The samples were then incubated in 1 mg/mL elastase (Sigma, cat. no. E0258) in DEPC-PBS at 25°C, with gentle mixing every 15 min. After incubation, the samples were centrifuged at 600 × g for 15 min at 4°C and resuspended in cold DEPC-PBS. They were then filtered through 70 µm filters and sorted using a FACS Aria II Sorter (BD Biosciences, USA). Three biological replicates were performed. For subsequent analyses, samples were lysed with a lysis buffer, and total RNA was extracted using the Cell Total RNA Isolation Kit (FOREGENE, RE-03111) following the manufacturer’s instructions. Reverse transcription was performed with oligo dT using the PrimeScript RT Reagent Kit (Vazyme, R312-01), and the first-strand cDNA was diluted tenfold with water for real-time PCR analysis (Vazyme, Q711-02). Expression levels were quantified using the 2−ΔΔCT method and normalized to RpL15, with the control sample’s expression set to Primer sequences for qPCR are listed in Supplementary Table S3.

Dissected midguts of adult female Drosophila deposited in cold PBS. After dissection, remove the PBS and add a mixture of 4% paraformaldehyde and n-heptane (1:1) for 30 min at 25°C (room temperature). After fixation, the samples were washed twice with methanol for 5 min each. They were then subjected to three washes with PBS containing 0.1% Triton, each wash lasting 10 min. Then, overnight incubation of the midguts at 4°C was performed using primary antibodies diluted in wash buffer. The primary antibodies used in this study were listed in Supplementary Table S5.

The next day, the midguts were subjected to three washes with 0.1% PBST, each lasting 10–15 min. Subsequently, incubated with secondary antibodies and DAPI (Sigma) for 2–3 h at 25°C (Alexa 488 and Alexa 568, Invitrogen) were used at a 1:2,000 attenuation. Confocal imaging was conducted using a Leica TCS-SP8 microscope, and the results were analyzed by LAS X software.

R version 3.5.3 for RNA-seq analysis is available at R Project. The custom ImageJ for quantifying immunofluorescence can be accessed at ImageJ. Prism 7.0 (GraphPad), used for data analysis in this study, is available on the GraphPad website.

The datasets presented in this study can be found in online repositories. The names of the repository/repositories and accession number(s) can be found in the article/Supplementary Material.

The Supplementary Material for this article can be found online at: https://www.frontiersin.org/articles/10.3389/fcell.2024.1491740/full#supplementary-material

SUPPLEMENTARY TABLE S1 | The top 30 elevated and decreased genes of UPR in the Kaempferol supplement compared to the control in Figure 4.

SUPPLEMENTARY TABLE S2 | The genes are mainly studied UPR in Figure 4.

SUPPLEMENTARY TABLE S3 | Primers used for expression analyses and the corresponding gene names.

SUPPLEMENTARY TABLE S4 | Drosophila strains utilized in this study.

SUPPLEMENTARY TABLE S5 | The primary antibodies listed in this study.

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