Split Fluorescent Protein-Mediated Multimerization of Cell Wall Binding Domain for Highly Sensitive and Selective Bacterial Detection

AbbreviationsCBD

cell wall binding domain

FP1–10,

1st to 10th beta-strands of FP

FP11,

11th beta-strand of FP

LstBD

lysostaphin binding domain

PlyGBD

endolysin binding domain from γ phage

SA1BD

autolysin SA1 binding domain

HRP

horseradish peroxidase

SUMO

small ubiquitin-like modifier

IPTG

isopropyl-β-D-thiogalactoside

PMSF

phenylmethanesulfonyl fluoride

Ni-NTA

nickel nitrilotriacetic acid

PBS

phosphate-buffered saline

SPR

surface plasmon resonance

NPB

native purification buffer

SDS-PAGE

sodium dodecyl sulfate-polyacrylamide gel electrophoresis

Keywords

Split fluorescent protein

Cell wall binding domain

Bacteria detection

Enzymatic biosensors

Data Availability

Data will be made available on request.

© 2024 The Author(s). Published by Elsevier B.V.

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