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metadata.dc.identifier.doi: https://doi.org/10.56042/ijeb.v62i05.1768Title: Correlations between beclin-1 and transforming growth factor-β1 in letrozole induced polycystic ovary syndromeAuthors: Hamdy, Nermin M.
Ghanem, Hala M.
Mohamed, Mervat M.
Abd El-Rahman, Fawzia A.A.
Soliman, Ahmed F.Keywords: Autophagy;Estradiol;Follicle-stimulating hormone;Luteinizing hormone;Oxidative stress;Progesterone;Protein carbonyl content;Reactive oxygen species (ROS);Testosterone;Total antioxidant capacityIssue Date: May-2024Publisher: NIScPR-CSIR, IndiaAbstract: In the pathogenesis of polycystic ovary syndrome (PCOS), despite the importance of autophagy and transforming growth factor-β1 (TGF-β1), there is scarce information about their inter-relationship. Therefore, here we assessed the correlations between beclin-1, a cornerstone in autophagy, and TGF-β1 in a letrozole-induced PCOS rat model. Accordingly, a total of 45 female adult albino Wistar rats were randomly assigned into control, vehicle (carboxymethyl cellulose), and PCO groups. To establish the PCOS model, letrozole (1.0 mg/kg body wt., p.o.) was given once daily for three successive weeks. Circulating levels of luteinizing hormone, follicle-stimulating hormone, testosterone, estradiol, and progesterone were assayed along with ovarian total antioxidant capacity (TAC), protein carbonyl content (PCC), beclin-1 level, and TGF-β1 level. Ovarian morphology and ultrastructure were examined by hematoxylin and eosin staining and electron microscopy, respectively. Compared to control groups, hormonal levels and ovarian morphology in the letrozole-exposed animals indicated the successful construction of the PCOS model. Further, the PCO group exhibited an oxidative stress status reflected by a significant decrease in ovarian TAC and a significant elevation in the PCC. Moreover, ovarian beclin-1 and TGF-β1 levels were significantly increased with an enhancement of autophagy as revealed by electron microscopy. In multiple linear regression models, only TGF-β1 was observed in the final model where it explained the 62.3% variability of Beclin-1. In conclusion, the ovarian level of TGF-β1 might be a determinant factor of beclin-1 level in PCOS which may provide new insight into the pathophysiology and therapy of the disease.Page(s): 332-340ISSN: 0975-1009 (Online); 0019-5189 (Print)Appears in Collections:IJEB Vol.62(05) [May 2024]

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