Raposo G, Stoorvogel W. Extracellular vesicles: exosomes, microvesicles, and friends. J Cell Biol. 2013;200(4):373–83.

Article  CAS  PubMed  PubMed Central  Google Scholar 

van Niel G, D’Angelo G, Raposo G. Shedding light on the cell biology of extracellular vesicles. Nature Rev Mol Cell Biol. 2018;19(4):213–28.

Article  Google Scholar 

Doyle LM, Wang MZ. Overview of extracellular vesicles, their origin, composition, purpose, and methods for exosome isolation and analysis. Cells. 2019;8(7):727.

Article  CAS  PubMed  PubMed Central  Google Scholar 

Kourembanas S. Exosomes: vehicles of intercellular signaling, biomarkers, and vectors of cell therapy. Annu Rev Physiol. 2015;77(1):13–27.

Article  CAS  PubMed  Google Scholar 

Anand S, Samuel M, Kumar S, Mathivanan S. Ticket to a bubble ride: cargo sorting into exosomes and extracellular vesicles. Biochim Biophys Acta, Proteins Proteomics. 2019;1867(12):140203.

Article  CAS  PubMed  Google Scholar 

Farzanehpour M, Miri A, Alvanegh AG, Gouvarchinghaleh HE. Viral vectors, exosomes, and vexosomes: potential armamentarium for delivering CRISPR/Cas to cancer cells. Biochem Pharmacol. 2023;212:115555.

Article  CAS  PubMed  Google Scholar 

Sancho-Albero M, Medel-Martínez A, Martín-Duque P. Use of exosomes as vectors to carry advanced therapies. Rsc Advances. 2020;10(40):23975–87.

Article  CAS  PubMed  PubMed Central  Google Scholar 

Elsharkasy OM, Nordin JZ, Hagey DW, de Jong OG, Schiffelers RM, Andaloussi SEL, Vader P. Extracellular vesicles as drug delivery systems: why and how? Adv Drug Deliv Rev. 2020;159:332–43.

Article  CAS  PubMed  Google Scholar 

Batrakova EV, Kim MS. Using exosomes, naturally-equipped nanocarriers, for drug delivery. J Control Release. 2015;219:396–405.

Article  CAS  PubMed  PubMed Central  Google Scholar 

Zhang Y, Bi JY, Huang JY, Tang YN, Du SY, Li PY. Exosome: a review of its classification, isolation techniques, storage, diagnostic and targeted therapy applications. Int J Nanomed. 2020;15:6917–34.

Article  CAS  Google Scholar 

Bulcha JT, Wang Y, Ma H, Tai PWL, Gao G. Viral vector platforms within the gene therapy landscape. Curr Signal Transduct Ther. 2021;6(1):53.

Article  CAS  Google Scholar 

Scioli Montoto S, Muraca G, Ruiz ME. Solid lipid nanoparticles for drug delivery: pharmacological and biopharmaceutical aspects. Front Mol Biosci. 2020;7:587997.

Müller RH, Mäder K, Gohla S. Solid lipid nanoparticles (SLN) for controlled drug delivery – a review of the state of the art. Eur J Pharm Biopharm. 2000;50(1):161–77.

Article  PubMed  Google Scholar 

Shirley JL, de Jong YP, Terhorst C, Herzog RW. Immune responses to viral gene therapy vectors. Mol Ther Nucleic Acids. 2020;28(3):709–22.

Article  CAS  Google Scholar 

Mehta M, Bui TA, Yang X, Aksoy Y, Goldys EM, Deng W. Lipid-based nanoparticles for drug/gene delivery: an overview of the production techniques and difficulties encountered in their industrial development. ACS Mater Au. 2023;3:600–19.

Article  CAS  PubMed  PubMed Central  Google Scholar 

Huang L, Wan J, Wu Y, Tian Y, Yao Y, Yao S, et al. Challenges in adeno-associated virus-based treatment of central nervous system diseases through systemic injection. Life Sci. 2021;270:119142.

Article  CAS  PubMed  Google Scholar 

Théry C, Amigorena S, Raposo G, Clayton A. Isolation and characterization of exosomes from cell culture supernatants and biological fluids. Curr Protoc Cell Biol. 2006;30(1):3–22.

Zeringer E, Barta T, Li M, Vlassov AV. Strategies for isolation of exosomes. Cold Spring Harb Protoc. 2015;2015(4):319–23.

Article  PubMed  Google Scholar 

Zhang M, Jin K, Gao L, Zhang Z, Li F, Zhou F, Zhang L. Methods and technologies for exosome isolation and characterization. Small Methods. 2018;2(9):1800021.

Article  Google Scholar 

Momen-Heravi F, Balaj L, Alian S, Mantel P-Y, Halleck AE, Trachtenberg AJ, et al. Current methods for the isolation of extracellular vesicles. Biol Chem. 2013;394(10):1253–62.

Article  CAS  PubMed  PubMed Central  Google Scholar 

Liangsupree T, Multia E, Riekkola ML. Modern isolation and separation techniques for extracellular vesicles. J Chromatogr A. 2021;1636:461773.

Article  CAS  PubMed  Google Scholar 

Baranyai T, Herczeg K, Onódi Z, Voszka I, Módos K, Marton N, et al. Isolation of exosomes from blood plasma: qualitative and quantitative comparison of ultracentrifugation and size exclusion chromatography methods. PLoS One. 2015;10(12):e0145686.

Article  PubMed  PubMed Central  Google Scholar 

An M, Wu J, Zhu J, Lubman DM. Comparison of an optimized ultracentrifugation method versus size-exclusion chromatography for isolation of exosomes from human serum. J Proteome Res. 2018;17(10):3599–605.

Article  CAS  PubMed  PubMed Central  Google Scholar 

Cheruvanky A, Zhou H, Pisitkun T, Kopp JB, Knepper MA, Yuen PST, Star RA. Rapid isolation of urinary exosomal biomarkers using a nanomembrane ultrafiltration concentrator. Am J Physiol Renal Physiol. 2007;292(5):F1657–61.

Article  CAS  PubMed  Google Scholar 

Xu R, Greening DW, Rai A, Ji H, Simpson RJ. Highly-purified exosomes and shed microvesicles isolated from the human colon cancer cell line LIM1863 by sequential centrifugal ultrafiltration are biochemically and functionally distinct. Methods. 2015;87:11–25.

Article  CAS  PubMed  Google Scholar 

Greening DW, Xu R, Ji H, Tauro BJ, Simpson RJ. A protocol for exosome isolation and characterization: evaluation of ultracentrifugation, density-gradient separation, and immunoaffinity capture methods. Methods Mol Biol. 2015;1295:179–209.

Article  CAS  PubMed  Google Scholar 

Sharma P, Ludwig S, Muller L, Hong CS, Kirkwood JM, Ferrone S, Whiteside TL. Immunoaffinity-based isolation of melanoma cell-derived exosomes from plasma of patients with melanoma. J Extracell Vesicles. 2018;7(1):1435138.

Article  PubMed  PubMed Central  Google Scholar 

Jiang Z, Liu G, Li J. Recent progress on the isolation and detection methods of exosomes. Chem Asian J. 2020;15(23):3973–82.

Article  CAS  PubMed  Google Scholar 

Tauro BJ, Greening DW, Mathias RA, Ji H, Mathivanan S, Scott AM, Simpson RJ. Comparison of ultracentrifugation, density gradient separation, and immunoaffinity capture methods for isolating human colon cancer cell line LIM1863-derived exosomes. Methods. 2012;56(2):293–304.

Article  CAS  PubMed  Google Scholar 

Muller L, Hong CS, Stolz DB, Watkins SC, Whiteside TL. Isolation of biologically-active exosomes from human plasma. J Immunol Methods. 2014;411:55–65.

Article  CAS  PubMed  PubMed Central  Google Scholar 

Hiemstra TF, Charles PD, Gracia T, Hester SS, Gatto L, Al-Lamki R, et al. Human urinary exosomes as innate immune effectors. J Am Soc Nephrol. 2014;25(9):2017–27.

Article  CAS  PubMed  PubMed Central  Google Scholar 

Lai RC, Arslan F, Lee MM, Sze NSK, Choo A, Chen TS, et al. Exosome secreted by MSC reduces myocardial ischemia/reperfusion injury. Stem Cell Res J. 2010;4(3):214–22.

Article  CAS  Google Scholar 

Liu F, Vermesh O, Mani V, Ge TJ, Madsen SJ, Sabour A, et al. The exosome total isolation chip. ACS Nano. 2017;11(11):10712–23.

Article  CAS  PubMed  PubMed Central  Google Scholar 

Gámez-Valero A, Monguió-Tortajada M, Carreras-Planella L, Marcel-la F, Beyer K, Borràs FE. Size-exclusion chromatography-based isolation minimally alters extracellular vesicles’ characteristics compared to precipitating agents. Sci Reports. 2016;6:33641.

Sidhom K, Obi PO, Saleem A. A review of exosomal isolation methods: is size exclusion chromatography the best option? Int J Mol Sci. 2020;21(18):6466.

Vaidyanathan R, Naghibosadat M, Rauf S, Korbie D, Carrascosa LG, Shiddiky MJA, Trau M. Detecting exosomes specifically: a multiplexed device based on alternating current electrohydrodynamic induced nanoshearing. Anal Chem. 2014;86(22):11125–32.

Article  CAS  PubMed  Google Scholar 

Zarovni N, Corrado A, Guazzi P, Zocco D, Lari E, Radano G, et al. Integrated isolation and quantitative analysis of exosome shuttled proteins and nucleic acids using immunocapture approaches. Methods. 2015;87:46–58.

Article  CAS  PubMed  Google Scholar 

Bruce TF, Slonecki TJ, Wang L, Huang S, Powell RR, Marcus RK. Exosome isolation and purification via hydrophobic interaction chromatography using a polyester, capillary-channeled polymer fiber phase. Electrophoresis. 2019;40:571–81.

Article  CAS  PubMed  Google Scholar 

Wang L, Bruce TF, Huang S, Marcus RK. Isolation and quantitation of exosomes isolated from human plasma via hydrophobic interaction chromatography using a polyester, capillary-channeled polymer fiber phase. Anal Chim Acta. 2019;1082:186–93.

Article  CAS  PubMed  Google Scholar