Senolytic effects of berberine protects fibroblasts cells against UV induced ageing
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https://doi.org/10.56042/ijeb.v62i03.803Title: Senolytic effects of berberine protects fibroblasts cells against UV induced ageingAuthors:
Sunilkumar, Athira RajiAnitha, Blessan VijayakumarShylaja, Karthika SajuBindhu, NeethuSheeja, Niranjana AjayakumarVijayanathan, LekshmiVijayakumar, NithinRamachandran, RajeshKeywords: Comet assay;DNA damage;Photoaging;Photochemoprotection;Radiation;Reactive oxygen species (ROS);Senescence;Ultraviolet raysIssue Date: Mar-2024Publisher: NIScPR-CSIR,IndiaAbstract: The gradual and irreversible pathophysiological process of ageing escalates many chronic diseases which may lead to
disability and ultimately death in elderly individuals. Premature ageing is largely associated with DNA damages similar to
the ones associated with UV-induced photoaging and the protective effects of phytochemicals as inhibitors of cellular
senescence is an area of increased research significance. In the present study, we tried to determine the UV protective and
senolytic effects of berberine in experimental ageing models. L929 cells were subjected to UV treated and treated with
different concentrations of berberine from 3.1 to 25 μg.mL-1. The effect of berberine on intracellular reactive oxygen species
(ROS) generation was determined by 2'-7'-dichlorodihydrofluorescein diacetate (DCFDA) staining. The DNA damages were
measured by comet assay and senescence was determined using galactosidase staining. The methylthiazolyldiphenyltetrazolium
bromide (MTT) results confirmed the berberine-associated protection in L929 cells with significant
improvement in cellular morphology. UV exposure significantly increased ROS generation and was decreased in groups cotreated
with berberine. UV irradiation resulted in increased tail length whereas co-treatment with berberine significantly
reduced DNA damage. X gal staining confirmed the senolytic effects of berberine. The experimental findings confirm that
berberine can limit the oxidative injury and DNA damages produced by UV rays which inturn find applications in limiting
ageing and associated metabolic diseases.Page(s): 187-191ISSN: 0975-1009 (Online); 0019-5189 (Print)Appears in Collections:
IJEB Vol.62(03) [March 2024]Items in NOPR are protected by copyright, with all rights reserved, unless otherwise indicated.
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