Traditional botanical knowledge across all use categories

In total, 246 plant species belonging to 53 families were reported to be known by the respondents in western Ladakh in contrary to other studies wherein 232 plants species belonging to 38 families were also documented from Eastern Ladakh [30]. Batool et al. have documented 176 medicinal plants belonging to 45 families from Trans Himalayan region of Ladakh [31]. Asteraceae (35) was the most dominant family, followed by Leguminosae (16), Ranunculaceae (14), Poaceae (11), Rosaceae (11), Gentianaceae (10), Polygonaceae (10), Amaranthaceae (9), Apiaceae (9) and Boraginaceae (9). These families have been recorded to be dominant in other studies [32,33,34]. From all the uses recorded in this study, maximum number of species was reported in the medicinal (23%), followed by fodder category (22%), ornamental (15%), food (15%), fuel wood (10%), religious (5%) and dye (4%). The category “other” (6%) included species used for making agricultural implements, sports appliances, artifacts, etc. Figure 2 shows the comparison of use category of plants across three valleys in Western Ladakh.

Fig. 2

Number of wild plant species used for different purposes across the valleys

The highest number of plants was cited by the locals in Suru (37%) followed by Wakha-chu (33%) and Lower Indus valley (30%).

Figure 3 shows the common species across the three valleys. The comparative information given by the communities in Suru, Wakha-chu and Lower Indus valley shows that there is variation in the knowledge of people living in three different valleys. Food and fodder were the most prominent use categories known by the people of the Lower Indus, while medicinal and fodder plants dominate the knowledge of people of the Suru valley. People in the Wakha-chu valley have more knowledge of medicinal and ornamental plants than the plants of other use categories. Multidimensional scaling shows the categories which were similar to each other were close and which were not related to each other were segregated (Fig. 4).

Fig. 3

Venn diagram showing the number of common and unique medicinal plants across three valleys

Fig. 4

Similarities/dissimilarities among different use categories of plants

Important plants in different use categoriesFood

Wild edible plants are an important constituent of traditional diets in Western Ladakh. These wild plants provide the majority of dietary requirement of proteins, sugars, vitamins and minerals [35, 36]. There are 84 plant species used as food in the study area. These plants were consumed as vegetable, salad, beverages and fruits. The dependency on wild plants as a source of food was higher in the isolated community of the Lower Indus valley as compared to the other two valleys. Earlier, people used to store sun-dried food collected during summers to use during the winters. However, during recent years such practices have ceased due to increased accessibility in markets. Development has not affected the dependency of people on wild edible plants, which has rather increased.

The maximum dependency on wild plants, i.e., 63 edible species, from the isolated community of the Lower Indus, 58 species from the Suru and 51 species from the Wakha-chu valley, has been reported. In the Lower Indus valley, the fruits of Prunus domestica (Phating) were the most popular food, eaten as meals, fruit, used as face pack, oil extraction and as beverages. The Lower Indus valley is famous for its traditional wine, which is popularly known as grun-chang (grape wine), prepared from grapes Vitis sp., which are only found in the Lower Indus valley. The other traditional beverage includes Chang and Aarak. These were prepared from barley and were popular among the Buddhist communities. Chang was prepared during religious and other celebrations and is considered auspicious. Rheum species (Lachu and khakol) were eaten raw while Allium species were used as a spice. They were also used to prepare a delicious traditional soup known as “Thukpa.” Urtica hyperborea (Zachot), Thymus linearis (Tumburu) and Capparis spinosa (Kapra) were dried and used as vegetable in traditional delicacy. Rosa webbiana (Pilli) and Lepidium latifolium seeds were ground with wheat flour to bake nutritious traditional bread (pongpong, khambir, tuk-tuk). Young shoots of Hippophae rhamnoides (tsetalulu), Potentilla anserina (toma) and Rosa webbiana (Sai-mendok) were used for making butter tea. More than half 71% (382) of the people interviewed in the three valleys use traditional food as their diet out of which 61% still consumes traditional food regularly (twice a day) and 32% consumes it once a day. Furthermore, the diet of the people has presumably undergone considerable changes, with the government providing food provisions such as sugar and rice at subsidized rates. Most of these changes have been accompanied by an increasing dependence on cash, integration with cash markets.

Tulipa stellata (Kapi-chong) species was recorded only from the Suru and in a parts of the Wakha-chu valley. Plants recorded from the interviews were mostly dependent on the availability of the species occurring in the area. In the Lower Indus valley, there was a difference in species ranking and citation. The top ten species ranked higher were not the frequently cited species. Medicago sativa (Ole), Ephedra gerardiana (Tsepat), Arnebia euchroma (Demok) and Morus alba (Osey) were the most cited species which were not listed among the ten most cited species. In the Suru valley, the ten highest priority plants based on average ranking were similar to the most cited species list, except Mentha longifolia (Phololing) and Bergenia stracheyi (Shapur). This was also the case in the Wakha-chu valley, except for Allium carolinianum (Skotse).

Fodder

Farmers do not feed their livestock during summers because it is possible to rear them on free range. Almost all respondents of Western Ladakh (95%) rear livestock and their livelihood are primarily dependent on agro-pastoral activity. The study revealed that there were 5877 livestock in Western Ladakh including 1554 cattle, 133 yaks, 615 donkeys and 61 horses (interview). Cattle and other livestock were reared essentially for household purposes. The majority of people use livestock milk to make butter, which was used for making salt tea, a very popular stimulant. In the Lower Indus valley, cow milk was prohibited, and so, they use livestock milk substituting the cow milk.

There were 123 species used as fodder from Western Ladakh, 83 species reported from Suru, 74 species from the Lower Indus and 54 from the Wakha-chu valley. Most of these species were collected from the wild. Only Medicago sativa and Avena spp. were cultivated. Some of the plant species were collected, dried and stored for winter. 68% of the people were still dependent on the wild for their fodder requirements.

In the Lower Indus valley, there was difference of two species between higher-ranking plants and most cited plants. Allium tortosum and Rheum webbianum were the most cited species but were not ranked high under the top priority species. In the Suru valley, the ten high priority plants based on average ranking were similar to the most cited species list, except Arnebia euchroma and Trifolium repens. In the Wakha-chu valley also, the ten high priority plants based on average ranking were similar to the most cited species list, except Rumex patientia, which was different from the most cited species list.

Fuel wood

In Western Ladakh 89% (483 households) of the total households surveyed were dependent on fuel wood for their major household activities. Despite the fact that 97% of the surveyed households have LPG (liquid petroleum gas, used for cooking) connection, but they were nevertheless dependent on fuel wood. Fuel wood is one of the important contrivances of all the communities in Ladakh. There were many species that could potentially be used as “fuel wood.” However, only a few of them, bearing unique characteristics, were sought after by the people. Of all the categories, fuel wood is highly utilized throughout the year for heating rooms during the harsh winters, cooking, roasting of grains to obtain flour. Juniperus semiglobosa (shukpa), Betula utilis (Stakpa) and Myricaria elegans (Umbu) were the most preferred fuel wood in the Lower Indus, Wakha-chu and Suru valleys, respectively. Other frequently used sources of fuel wood were Salix daphanoides, Acantholimon lycopodioides (Longzey), Artemisia spp. (Burtse) and Rosa webbiana (Sai). A total of 64 species were recorded as fuel wood from Western Ladakh. The ethnic groups have reported 23 species in the Lower Indus, 38 species in Wakha-chu and 40 species in Suru valley. Fuel wood was collected from the wild. The extraction of fuel wood varies from 50 to 100 kg per family. Collection varies from valley to valley. There were certain rules made by the locals on collection of these resources from the wild, but these were usually not followed.

In the Lower Indus valley, there was a difference of one species between higher-ranking plants and most citation species. Populus sp. (Yerpa) was frequently cited but not listed under the top-ranking species. In the Suru valley, the ten most priority plants based on average ranking were similar to the most cited species list. In the Wakha-chu also, the ten high priority plants based on average ranking were similar to the most cited species list, except for Caragana versicolor (Tama, Brama).

Ritual

Like any other traditional society, people of Western Ladakh have a deep faith in spirituality which is reflected though their customs [37]. This is why they have a high affinity toward religious plants, which were used in everyday lives. Thirty-one species of plants were recorded which were exclusively used for the same purpose. In the Lower Indus 23 species, 11 from Suru and 20 from Wakha-chu valley were recorded as religious plants. These plants were used in all the religious rituals from birth to death. The monks, Shamans, Akhons and locals use them during several religious occasions [15]. Mostly religious plants were used either to please the deities or assure their support of human health and well-being or to drive out the malevolent spirits. The ritual plants were typically trees, shrubs or herbs. Arnebia euchroma (Demok or Sbrimok) has maximum citation in the Lower Indus and Wakha-chu valleys, while in the Suru valley Viola kunawurensis (Gunapsha) ranks first.

In certain rituals, different voodoo (storma) dolls were made from the branches of Salix tetrasperma or Myricaria elegans. These “voodoo-like dolls” were used to drive ghosts away, to cure illnesses and during funerals. Juniperus semiglobosa (Shukpa or Chilgi) was the most important plant for religious purposes. Its needle scaly leaves were used as incense before each ritual. Additionally, the leaves are considered an important contrivance for the Shamans (Lha/Lhamos) to follow the rituals performed by them. Other plants used for the same purpose were Waldheimia glabra (Palu) and Rosa webbiana. These were dried and mixed with Juniperus sp. and burned in an incense holder (Phoks). One of the mixtures used regularly by every household, locally called Sangs, prepared from tsampa (roasted barley grain) flour mixed with flowers of Tagetes erecta, Rosa webbiana or Waldhemia sp. This powder was stored in a container and put into the incense bowl (Phoks-por) every morning.

In the Lower Indus and Suru valleys, higher-ranking plants and most cited species were similar. However, there was a difference in the hierarchical order. In the Wakha-chu valley, plants based on average ranking were slightly different from the most cited species list Malus domestica (Ku-shu) was replaced by Salix alba (changma) as the most cited species.

Medicinal plants

This was the largest class with 120 plant species, most of which have multiple medicinal uses. Maximum number of medicinal plants was reported from the Wakha-chu valley (88 species) followed by the Suru (85 Species) and Lower Indus valleys (54 species). The diversity of species used as medicine was higher in the Wakha-chu and Suru valleys as compared to the Lower Indus valley. The utilization of the plants depends on their availability and accessibility. Summer and autumn were the best seasons for collection. Most people collect during the rearing of animals in the higher altitude areas. They always collect some plants, which were required for curing ailments. These medicinal plants were taken orally and topically in different forms such as powder, paste, ointment or decoction (liquid obtained from boiling of the medicinal plants in a solvent) and were prescribed by the Amchis [38]. Different types of preparations were used for different conditions and diseases. For example, Aconitum spp. was used for stomachache; the plant was used as a powder and as a decoction. The medicines most commonly used were Aconitum rotoundifolium (Boga) for stomachache and Nepeta longibracteata (Piyangku) for other gastrointestinal problems [39]. They also use these medicinal plants for curing animal diseases. Cicer microphyllum (Sari) used for mouth ulcer in cattle. The mites in livestock were removed with Stachys tibetica (Yakzes), and decoction was used as anti-mite. The study shows that Inula racemosa was the only medicinal plant, which was cultivated in fields. Hyoscyamus pusillus was another plant frequently used by the people for toothache.

In the Lower Indus valley, there was difference of one species between high-ranking plants and most cited plants. Rheum spiciforme was among the ten most cited species but was not listed under the top ten ranking species. In the Suru valley, the ten high priority plants, based on average ranking, were similar to the most cited species list, except for Arnebia euchroma. In the Wakha-chu valley, the ten high priority plants based on average ranking were similar to the most cited species list.

Dyes

People in Ladakh have traditionally been engaged in extraction, processing and preparation of dyes using barks, leaves, fruits and roots of plant. In Western Ladakh, 19 species of plants were recorded to be used as dyes. These were especially used for dying wool, food and hair. The most commonly used dye was Arnebia euchroma, which was a multipurpose dye plant used in every ritual. Before synthetic clothes were introduced into the area, locals wore only traditional wool attires, which were dyed with Arnebia euchroma (Demok). These attires were yarned from the wool of sheep. The leaves and flower of Rumex sp. and Rosa species also yield dyes, which give wool a blackish red color. The fastness or longevity of dyes depends on the use of a mordant and personal experience, which can bring refinement to a particular dye or specific preparation.

In the Lower Indus, Suru and Wakha-chu valleys, there was no difference between higher-ranking plants and most cited plants list. This means that the species cited most frequently were also ranked high in all the valleys.

Ornamental

People of Ladakh use various types of ornamental plants in their houses, monasteries and during religious and cultural occasions. Total 66 species of ornamental plants were used in the Wakha-chu, 41 in the Lower Indus and 56 in the Suru valley. The Brokpas of the Lower Indus have more affinity toward ornamental plants than the other two valleys. They wear flowers (as headgear) on every occasion. Ornamental plants were also used for worshiping, decoration and to welcome guests. The ornamental plants were also used to express love and care to their beloved ones. There was a folklore, which describes sending wildflowers (Puroh) from the high grazing land, where they take their cattle for rearing, to their families in the villages. This symbolizes the love and well-being of the person.

The species ranked higher were not the frequently cited species. Rosa webbiana and Geranium spp. were the most cited species but were not listed among the higher ranked species. In the Suru valley, the ten highest priority plants based on average ranking were similar to the most cited species list, except Rosa ecae. In the Wakha-chu valley also the ten highest priority plants based on average ranking were similar to the most cited species list, except Rosa webbiana and Saxifraga sp. which were different under the most cited species list.

Others

This category consists of plants, which were used for making tools, cooking utensils, containers and construction. Out of 75 plants documented, 21 species were used in the Lower Indus valley, 29 and 25 species in the Wakha-chu and Suru valleys, respectively. These plants were used for making various household artifacts such as brooms, utensils, baskets, measuring devices for grains, containers for chang and wooden barrels for storing grains. Local people carve the hard inner wood of certain species into mortars for grinding. They also design barley containers (zong) made from the Salix wood. Styipa sp. (sibskya), a typical grass was split, thinned and then woven into local strainers (chakma), which were used during the preparation of chang (local beer) and other daily household activities. Salix and Styipa twigswere also used for weaving baskets (Chepo) which were very popular in every household. Chepo (figure) was one of the assets, which was a part of women’s attire in early times in Ladakh. The stem of the Vitis sp. (grun) was the most preferred fiber used for making ropes in the Lower Indus valley.

Artemesia spp. (burtse) was used to make brooms. The wood of Betula utilis was the preferred material to make plows and agricultural implements. Though the use of wood for house construction was decreasing, wood flooring (shen) was still preferred throughout Ladakh. The flooring were made with Juglans regia (starga), Prunus sp. (Phating) and Salix sp. Sometimes even Populus nigra (yerpa) was also used for flooring. Traditional woodcarving was another very important utility of wood in Western Ladakh. The woods commonly used for this purpose were walnut and apricot. Juniper species were also used in making large containers called zem, used for making and storing local bear Chang. Ribes sp. (seth) and Rosa sp. (sai) were used for making arrows which were used during the festivals devoted to Archery. Corydalis sp. (Nya-tuk) was used for poisoning fish or fish traps.

The important species ranked higher were not the frequently cited species. Lonicera spp. and Salix tetrasperma were the most cited species but were not listed among the higher ranked species in the Lower Indus and Suru valleys.

Cultural importance indices

Table 2 shows the contribution of each use category to the total cultural importance (CI) index of the 30 most relevant and useful species in Western Ladakh. The folk species Arnebia euchroma “Demok” was the most culturally significant according to the CI index. It has a CI index value of 1.55 (FC = 442) citations (836 use reports).

Table 2 Cultural importance (CI) index of most relevant species of Western Ladakh with each use category

Arnebia euchroma was mainly used for religious purposes and dyes (CIRE = 0.47, CIDY = 0.47) or as firewood (CIFW = 0.11), fodder and medicine (CIFO = 0.15, CIMD = 0.15). The second species in the rank order was Juniperus semiglobosa L. (CI = 1.42). Several informants cited its use in six out of the eight categories. The most important use category was religious (CIRE = 0.68), which was also observed during the interview. Every household uses Juniper for most of the rituals. The other uses include fuel wood (CIFW = 0.47), other categories (tool making, furniture, artifacts, etc.) (CIOT = 0.24), ornamental (CIOR = 0.02) and medicine (CIMD = 0.01).

Each index aims to assess the cultural significance of plant species and is suitable for statistical testing. For comparison, we used data concerning plants traditionally used in the study area. Our results show a positive and significant correlation between the number of uses (NU) and the frequency of citation (FC) of the species. It seems to be a general rule that the more versatile a plant, the more widespread its usefulness. In addition, NU is highly influenced by the number of use categories in the study.

Comparing different indices

Table 3 shows a comparison with the other three indices described earlier in the methodology section, indicating species ranking based on each index and the three basic values of the study, i.e., frequency of citation (FC), use reports (UR) and number of uses (NU) for each species. As mentioned, except for FC, which only considers the spread of knowledge of useful plants (number of people that mention them), the other indices also consider multiplicity of use (number of use categories mentioned for a species).

Table 3 Indices and ranking of useful plants of Western Ladakh

There are considerable differences in species ranking yielded by the various indices set out in table. Although the first two species are the same in all of them, the order varies depending on the chosen index. The CI, RI and CV indices place Arnebia euchroma in first position because these two indices assign greater importance to the multiplicity of uses and the species was mentioned in a higher number of use categories (NU = 8). Artemisia spp. logically should be considered the most important as they predominate in the landscape and are mentioned by a higher number of informants.

Table 4 shows the Spearman correlations among all the variables. All the correlations are significant at P < 0.05 (n = 247), some being stronger than others. The correlations range from 0.76 to 0.99 (highly correlated). An interesting point that appears to corroborate these data is that the frequency of citation (FC) is not completely independent of use diversity. The correlation index between the FC and NU is quite high (0–76) meaning that a versatile species is more likely to be mentioned by a higher number of informants.

Table 4 Spearman rank order correlations among all variables: basic values and indicesTraditional knowledge across age, gender and ethnicity

Traditional knowledge based on the scores (to measure the knowledge), photographs and plant specimens were shown to the respondents and hypotheses were created. Unexpectedly, most of the plants had similar names in different valleys. Limitation was deficient vocabulary of plants, which encountered in all the three valleys. For example, multiple names were given for the same species, Medicago sp., for example ole, buksuk, namtse. Multiple names were also recorded for these plants. The number of plants in each use category given by a respondent varied depending on several factors, which were discussed below in detail.

H0 1 There was no significant difference between knowledge of people across different age groups (15–30, 31–50, above 51 years).Age

Analysis of variance between use categories and informant age groups indicated that the youngest informants group (15–30) had the lowest levels of knowledge (F = 174.4, df = 2 and P = 0.00 or P < 0.05), which can be attributed to a lack of interest in learning and practicing such knowledge. Difference in knowledge was found to be statistically significant for most of the use categories (P = 0.021). Informants that identified most of the plants belonged to the 41–50 age groups, suggesting that knowledge of plant species is only concentrated among the veterans. No significant differences (P = 0.10) were found in the use categories of dye and religion. Religious and dye plants are both contemporary uses. Both young and old unlike other uses use them. One possible explanation for this is that there were fewer plants used by the people in this category and their use was frequent across the valleys.

Age-related divergences in knowledge might either be an indication of a gradual erosion of knowledge. Formal education, up to some extent, has changed the way of livelihood of local people and restricted their interaction with local biophysical and cultural environment. Another reason of less traditional knowledge among the youngsters was increasing opportunities in different fields and acculturation. Older people are more experienced and have had greater contact with plant resources through exchange of knowledge. Older people prepare home remedies for themselves and for younger people, which favors the retention of knowledge.

There was a significant difference in knowledge levels across all age classes in all three valleys. Lower Indus valley (F = 1.60, df = 2 and P < 0.05), Suru valley (F = 81.14, df = 2 and P < 0.02) and Wakha-chu valley (F = 127.8, df = 2 and P < 0.05). However, knowledge varies between age classes for different categories in each valley: food (F = 149.61, df = 2 and P < 0.0001), fodder (F = 129.95, df = 2 and P < 0.05), fuel wood (F = 48.89, df = 2 and P < 0.0001), ornamental (F = 83.61, df = 2 and P < 0.0001), medicine (F = 96.34, df = 2 and P < 0.0001), dye (F = 61.44, df = 2 and P < 0.0001), Religious (F = 42.78, df = 2 and P < 0.0001), other (F = 38.94, df = 2 and P < 0.0001). This difference in knowledge among different age classes in the Lower Indus valley is shown in Table 5.

Table 5 Traditional knowledge score (mean ± SE) across different age class and gender in Western LadakhH0 2 There was no significant difference between the knowledge of people across genderGender

Differences between men and women’s knowledge are often related to divergences in their daily activities and to their divergent domains of responsibility [40,41,42,43]. Gender-related differences between men and women in ethnobotanical knowledge were often ascribed to the division of household responsibilities, labor and expertise, control and interests at the intra-household, inter-household and community levels. Result (Table 5) shows that across the valleys there was no difference in knowledge between men and women. Men have an average knowledge score (of useful plant species) of 109.18 ± 2.7 (mean ± standard error), whereas women have an average score of 111.68 ± 2.8. No significant differences were found between the two groups (Tukey’s test, F = 0.413, df = 1 and P = 0.52). The null hypothesis (Ho) was accepted. However, knowledge of plant species under different categories shows those women knew of significantly more food plants than men. In the study, in contrast to a common perception that men possess greater knowledge of fodder and fuel wood plants due to greater participation in activities related to natural resources, women too were equally involved in activities linked to the use of plant resources. It appeared that women possessed a level of knowledge, which was not restricted simply to that of plants that are directly related to their activities in the house and raising children.

H0 3 There was no significant difference between the knowledge of people (number of plants) across different valleys

We rejected the null hypothesis (Ho) as we found evidence that the people of the Lower Indus valley were significantly more knowledgeable than those of the Suru and the Wakha-chu communities. This difference was due to the behavioral distinctions between the ethnic groups. They were closer to nature and their history shows that their religious beliefs and rituals were originally in essence demonolatry, ancestor worship and nature worship [10]. The Lower Indus community was isolated from other parts of Ladakh since 1979 and they were more attached to nature and thus like to incorporate nature in their lives by simply what they wear as headgear, which was one of the important assets of their personality. Hence, they were closer to nature than the other two groups (F = 90.7, df = 2, P = 0.0001) shown in Table 6.

Table 6 Knowledge score (mean ± standard error) across different valleys in Western Ladakh

But it was interesting to note that there was no significant difference between the knowledge of the people of the Suru and that of the Wakha-chu valley (F = 0.29, df = 1, P = 0.587). They almost have similar knowledge of plants. The reason may be due to intercultural relationships between the valleys, which were relatively close to each other. Plants have great importance in all the valleys and locals were dependent on them for their daily requirements. However, across all age categories in the three valleys there was a significant difference in knowledge of plants (age class I: F = 18.06, df = 2, P = 0.0001); age class II: F = 81.98, df = 2, P = 0.0001; and age class III: F = 153.60, df = 2, P = 0.0001).

Knowledge across the different use categories in three valleys was also significant.

Another important aspect affecting TK is the dependency of people on locally available resources. Indigenous knowledge of plant species is often strong for species that are in common use by the local community. In more remote areas, people generally rely more on local resources, and with increasing dependency on external resources through access to roads, markets and other modern goods and services, knowledge may decline considerably. However, this tendency can be reversed when other factors such as environment, sociocultural identity and settlement history come into play [44, 45].

Socioeconomic and other factors of knowledge

To determine the influence of socioeconomic and other factors on the knowledge of people, different explanatory variables were selected. These variables were age, gender, religion, valley affiliation or ethnicity and socioeconomic variables such as distance to town, education, tourism, occupation and income. Analysis was performed for total knowledge as well as for each use category separately. The result of the stepwise multiple regressions showed that the important factor that influenced the traditional knowledge in Western Ladakh was the age of the informant. This was true and statistically significant both when the entire data set was analyzed (Table 7) and when it was broken down between three valleys (Lower Indus, Suru and Wakha-chu) and also when it was broken down between use categories (food, fodder, fuel wood, ornamental, medicine, dye, religious and other).

Table 7 Different variables and impact of these variables in Western Ladakh

Other socioeconomic variables that influence plant knowledge when the entire dataset was analyzed were valley affiliation, distance, religion and education (R2 = 0.68, d F = 5, F = 232.79 and P < 0.05) (Table 7).

The prime importance of the informants’ age reflects dwindling of knowledge in Western Ladakh and across different valleys, respectively. It may also indicate that age encompasses experience or accumulation of knowledge through the course of a lifetime.

The second variable that influences knowledge was valley affiliation, which was ethnicity. It may also indicate that valley affiliation includes a number of aspects that functions in parallel, such as marginalization or isolation of the villages in the valley, the condition of the ecosystems available to villages and the ethnic condition of the valley. The effect of the residence village on traditional knowledge showed that the choice of plant resources purposes might be influenced by the cultural characteristics of the informants.

The third variable, which influenced the knowledge of people, was distance from the main town, Kargil. This observation was used to test the null hypotheses that modernization has caused loss of traditional knowledge on local level. People living in the remote areas or away from the main town have least access to goods and they totally depend on the natural resources available in their neighborhood. Significant but negative relationships were found between knowledge and increasing distance from interviewee’s village to Kargil. It was observed that while other ethnobotanical knowledge was being lost slowly, medicinal plant knowledge has been disappearing rapidly over an extensive period of acculturation. Nearly a decade later after the study of Kachroo [46], there is extremely limited ethnobotanical knowledge present in the province. The result supports the argument that medicinal plant knowledge is particularly vulnerable to modernization.

The fourth variable, which influences the knowledge in Western Ladakh, was religion. The practice of a religion which include rituals, sermons, commemoration or veneration of deity, gods or goddesses, sacrifices, festivals, feasts, trance, initiations, funerary, services, matrimonial services, meditation, prayer, music, art, dance, public service or other aspects of human culture. It was observed that a Buddhist respondent knows more plants compared to Muslims.

The fifth factor influencing knowledge was education, which was negatively correlated to the informant knowledge of plants. It was found that people who were illiterate, have more traditional knowledge compared to educated people, because mostly illiterate people were associated with agricultural practices, resources extraction and other traditional activities.

Comparison among use categories

Traditional knowledge of different use categories had different responses to the variables (Table 8).

Table 8 Traditional knowledge score of different use categories across variables influencing them

The knowledge of food, age and distance shows a positive relationship while male respondents had a negative relationship (R2 = 0.54, F = 7.246, df = 1, P = 0.007). In case of fodder, age had a positive relationship while male respondents showed a negative relationship (R2 = 0.35, F = 4.646, df = 1, P = 0.032). In fuelwood knowledge, age, distance to town and tourism had a positive relationship and male respondent had negative relationship (R2 = 0.50, F = 4.638, df = 1, P = 0.032). In ornamental category, age and religion (Buddhist) have a positive relationship while tourism and male respondents had a negative relationship (R2 = 0.46, F = 4.449 df = 1, P = 0.035). In the medicinal plant category, age and distance to town show a positive relationship (R2 = 0.35, F = 16.619, df = 1, P = 0.00). In the case of dye, age, distance to town and tourism show a positive while education shows a negative relationship (R2 = 0.27, F = 7.180 df = 1, P = 0.008). In religious plant category, distance, age and religion (Buddhist) shows a positive and tourism shows a negative influence (R2 = 0.43, F = 5.212, df = 1, P = 0.023). In the other category, age shows a positive and distance and occupation of people show a negative relationship (R2 = 0.59, F = 0.353, df = 1, P = 0.040).