Kastner, B., Will, C. L., Stark, H. & Lührmann, R. Structural insights into nuclear pre-mRNA splicing in higher eukaryotes. Cold Spring Harb. Perspect. Biol. 11, a032417 (2019).
Article CAS PubMed Central PubMed Google Scholar
Wilkinson, M. E., Charenton, C. & Nagai, K. RNA splicing by the spliceosome. Annu. Rev. Biochem. 89, 359–388 (2020).
Article CAS PubMed Google Scholar
Wan, R., Bai, R., Zhan, X. & Shi, Y. How is precursor messenger RNA spliced by the spliceosome? Annu. Rev. Biochem. 89, 333–358 (2020).
Article CAS PubMed Google Scholar
Will, C. L. & Lührmann, R. Spliceosome structure and function. Cold Spring Harb. Perspect. Biol. 3, a003707 (2011).
Article CAS PubMed Central PubMed Google Scholar
Vorländer, M. K., Pacheco-Fiallos, B. & Plaschka, C. Structural basis of mRNA maturation: time to put it together. Curr. Opin. Struct. Biol. 75, 102431 (2022).
Grainger, R. J. & Beggs, J. D. Prp8 protein: at the heart of the spliceosome. RNA 11, 533–557 (2005).
Article CAS PubMed Central PubMed Google Scholar
Golas, M. M. et al. 3D cryo-EM structure of an active step I spliceosome and localization of its catalytic core. Mol. Cell 40, 927–938 (2010).
Article CAS PubMed Google Scholar
Bach, M. & Lührmann, R. Protein–RNA interactions in 20S U5 snRNPs. Biochim. Biophys. Acta 1088, 139–143 (1991).
Article CAS PubMed Google Scholar
Laggerbauer, B. et al. The human U5 snRNP 52K protein (CD2BP2) interacts with U5-102K (hPrp6), a U4/U6.U5 tri-snRNP bridging protein, but dissociates upon tri-snRNP formation. RNA 11, 598–608 (2005).
Article CAS PubMed Central PubMed Google Scholar
Liu, Y.-C. & Cheng, S.-C. Functional roles of DExD/H-box RNA helicases in pre-mRNA splicing. J. Biomed. Sci. 22, 54 (2015).
Article PubMed Central PubMed Google Scholar
Plaschka, C., Newman, A. J. & Nagai, K. Structural basis of nuclear pre-mRNA splicing: lessons from yeast. Cold Spring Harb. Perspect. Biol. 11, a032391 (2019).
Article CAS PubMed Central PubMed Google Scholar
Bialkowska, A. & Kurlandzka, A. Proteins interacting with Lin 1p, a putative link between chromosome segregation, mRNA splicing and DNA replication in Saccharomyces cerevisiae. Yeast 19, 1323–1333 (2002).
Article CAS PubMed Google Scholar
Klimešová, K. et al. TSSC4 is a component of U5 snRNP that promotes tri-snRNP formation. Nat. Commun. 12, 3646 (2021).
Article ADS PubMed Central PubMed Google Scholar
Bergfort, A. et al. The intrinsically disordered TSSC4 protein acts as a helicase inhibitor, placeholder and multi-interaction coordinator during snRNP assembly and recycling. Nucleic Acids Res. 50, 2938–2958 (2022).
Article CAS PubMed Central PubMed Google Scholar
Boon, K. L. et al. prp8 mutations that cause human retinitis pigmentosa lead to a U5 snRNP maturation defect in yeast. Nat. Struct. Mol. Biol. 14, 1077–1083 (2007).
Article CAS PubMed Central PubMed Google Scholar
Weber, G. et al. Structural basis for dual roles of Aar2p in U5 snRNP assembly. Genes Dev. 27, 525–540 (2013).
Article CAS PubMed Central PubMed Google Scholar
Laggerbauer, B., Achsel, T. & Lührmann, R. The human U5-200kD DEXH-box protein unwinds U4/U6 RNA duplices in vitro. Proc. Natl Acad. Sci. USA 95, 4188–4192 (1998).
Article ADS CAS PubMed Central PubMed Google Scholar
Plaschka, C., Lin, P.-C., Charenton, C. & Nagai, K. Prespliceosome structure provides insights into spliceosome assembly and regulation. Nature 559, 419–422 (2018).
Article ADS CAS PubMed Central PubMed Google Scholar
Charenton, C., Wilkinson, M. E. & Nagai, K. Mechanism of 5′ splice site transfer for human spliceosome activation. Science 364, 362–367 (2019).
Article ADS CAS PubMed Central PubMed Google Scholar
Zhan, X., Yan, C., Zhang, X., Lei, J. & Shi, Y. Structures of the human pre-catalytic spliceosome and its precursor spliceosome. Cell Res. 28, 1129–1140 (2018).
Article CAS PubMed Central PubMed Google Scholar
Agafonov, D. E. et al. Molecular architecture of the human U4/U6.U5 tri-snRNP. Science 351, 1416–1420 (2016).
Article ADS CAS PubMed Google Scholar
Bell, M., Schreiner, S., Damianov, A., Reddy, R. & Bindereif, A. p110, a novel human U6 snRNP protein and U4/U6 snRNP recycling factor. EMBO J. 21, 2724–2735 (2002).
Article CAS PubMed Central PubMed Google Scholar
Nielsen, T. K., Liu, S., Lührmann, R. & Ficner, R. Structural basis for the bifunctionality of the U5 snRNP 52K protein (CD2BP2). J. Mol. Biol. 369, 902–908 (2007).
Article CAS PubMed Google Scholar
Galej, W. P., Oubridge, C., Newman, A. J. & Nagai, K. Crystal structure of Prp8 reveals active site cavity of the spliceosome. Nature 493, 638–643 (2013).
Article ADS CAS PubMed Central PubMed Google Scholar
Jehle, S. et al. A human kinase yeast array for the identification of kinases modulating phosphorylation-dependent protein–protein interactions. Mol. Syst. Biol. 18, e10820 (2022).
Article CAS PubMed Central PubMed Google Scholar
Weber, G. et al. Mechanism for Aar2p function as a U5 snRNP assembly factor. Genes Dev. 25, 1601–1612 (2011).
Article CAS PubMed Central PubMed Google Scholar
Liu, S., Rauhut, R., Vornlocher, H. P. & Lührmann, R. The network of protein-protein interactions within the human U4/U6.U5 tri-snRNP. Rna 12, 1418–1430 (2006).
Article CAS PubMed Central PubMed Google Scholar
Schreib, C. C. et al. Functional and biochemical characterization of Dib1’s role in pre-messenger RNA splicing. J. Mol. Biol. 430, 1640–1651 (2018).
Article CAS PubMed Central PubMed Google Scholar
Mayeda, A. & Krainer, A. R. Preparation of HeLa cell nuclear and cytosolic S100 extracts for in vitro splicing. Methods Mol. Biol. 118, 309–314 (1999).
Kastner, B. et al. GraFix: sample preparation for single-particle electron cryomicroscopy. Nat. Methods 5, 53–55 (2008).
Article CAS PubMed Google Scholar
Tegunov, D. & Cramer, P. Real-time cryo-electron microscopy data preprocessing with Warp. Nat. Methods 16, 1146–1152 (2019).
Article CAS PubMed Central PubMed Google Scholar
Scheres, S. H. W. RELION: implementation of a Bayesian approach to cryo-EM structure determination. J. Struct. Biol. 180, 519–530 (2012).
Article CAS PubMed Central PubMed Google Scholar
Punjani, A., Rubinstein, J. L., Fleet, D. J. & Brubaker, M. A. cryoSPARC: algorithms for rapid unsupervised cryo-EM structure determination. Nat. Methods 14, 290–296 (2017).
Article CAS PubMed Google Scholar
Jamali, K., Kimanius, D. & Scheres, S. ModelAngelo: automated model building in cryo-EM maps. Preprint at bioRxiv https://doi.org/10.1101/2023.05.16.541002 (2023).
Goddard, T. D. et al. UCSF ChimeraX: meeting modern challenges in visualization and analysis. Protein Sci. 27, 14–25 (2018).
Article CAS PubMed Google Scholar
Evans, R. et al. Protein complex prediction with AlphaFold-Multimer. Preprint at bioRxiv https://doi.org/10.1101/2021.10.04.463034 (2022
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