Barb AW et al (2012) NMR characterization of immunoglobulin G fc glycan motion on enzymatic sialylation. Biochemistry 51:4618–4626
Article CAS PubMed Google Scholar
Barb AW, Falconer DJ, Subedi GP (2019) The preparation and solution NMR spectroscopy of human glycoproteins is accessible and rewarding. Methods Enzymol 614:239–261
Article CAS PubMed Google Scholar
Barbieri L, Luchinat E, Banci L (2016) Characterization of proteins by in-cell NMR spectroscopy in cultured mammalian cells. Nat Protoc 11:1101–1111
Article CAS PubMed Google Scholar
Callaway E (2020) Revolutionary cryo-EM is taking over structural biology. Nature 578:201
Article CAS PubMed Google Scholar
Chalmers GR et al (2019) NMR resonance assignment methodology: characterizing large sparsely labeled glycoproteins. J Mol Biol 431:2369–2382
Article CAS PubMed PubMed Central Google Scholar
Delaglio F et al (1995) NMRPipe: a multidimensional spectral processing system based on UNIX pipes. J Biomol NMR 6:277–293
Article CAS PubMed Google Scholar
Dutta A, Saxena K, Schwalbe H, Klein-Seetharaman J (2012) Isotope labeling in mammalian cells. Methods Mol Biol 831:55–69
Article CAS PubMed PubMed Central Google Scholar
Ereno-Orbea J et al (2017) Molecular basis of human CD22 function and therapeutic targeting. Nat Commun 8:764
Article PubMed PubMed Central Google Scholar
Feng L, Lee HS, Prestegard JH (2007) NMR resonance assignments for sparsely 15 N labeled proteins. J Biomol NMR 38:213–219
Article CAS PubMed Google Scholar
Johnson BA (2004) Using NMRView to visualize and analyze the NMR spectra of macromolecules. Methods Mol Biol 278:313–352
Jumper J et al (2021) Highly accurate protein structure prediction with AlphaFold. Nature 596:583–589
Article CAS PubMed PubMed Central Google Scholar
Luchinat E, Barbieri L, Banci L (2017) A molecular chaperone activity of CCS restores the maturation of SOD1 fALS mutants. Sci Rep 7:17433
Article PubMed PubMed Central Google Scholar
Matthews DE (2020) Review of lysine metabolism with a focus on humans. J Nutr 150:2548S–2555S
Moremen KW, Tiemeyer M, Nairn AV (2012) Vertebrate protein glycosylation: diversity, synthesis and function. Nat Rev Mol Cell Biol 13:448–462
Article CAS PubMed PubMed Central Google Scholar
Nkari WK, Prestegard JH (2009) NMR resonance assignments of sparsely labeled proteins: amide proton exchange correlations in native and denatured states. J Am Chem Soc 131:5344–5349
Article CAS PubMed PubMed Central Google Scholar
Ollerenshaw JE, Tugarinov V, Kay LE (2003) Methyl TROSY: explanation and experimental verification. Mag Res Chem 41:843–852
Pervushin K, Riek R, Wider G, Wuthrich K (1997) Attenuated T2 relaxation by mutual cancellation of dipole–dipole coupling and chemical shift anisotropy indicates an avenue to NMR structures of very large biological macromolecules in solution. Proc Natl Acad Sci U S A 94:12366–12371
Article CAS PubMed PubMed Central Google Scholar
Riek R, Wider G, Pervushin K, Wuthrich K (1999) Polarization transfer by cross-correlated relaxation in solution NMR with very large molecules. Proc Natl Acad Sci U S A 96:4918–4923
Article CAS PubMed PubMed Central Google Scholar
Sastry M, Bewley CA, Kwong PD (2015) Effective isotope labeling of proteins in a mammalian expression system. Methods Enzymol 565:289–307
Article CAS PubMed PubMed Central Google Scholar
Subedi GP, Barb AW (2015) The structural role of antibody N-glycosylation in receptor interactions. Structure 23:1573–1583
Article CAS PubMed PubMed Central Google Scholar
Subedi GP, Johnson RW, Moniz HA, Moremen KW, Barb A (2015) High yield expression of recombinant human proteins with the transient transfection of HEK293 cells in suspension. J Vis Exp. https://doi.org/10.3791/53568
Article PubMed PubMed Central Google Scholar
Takeuchi H, Haltiwanger RS (2014) Significance of glycosylation in notch signaling. Biochem Biophys Res Commun 453:235–242
Article CAS PubMed PubMed Central Google Scholar
Venters RA, Farmer BT 2nd, Fierke CA, Spicer LD (1996) Characterizing the use of perdeuteration in NMR studies of large proteins: 13 C, 15 N and 1H assignments of human carbonic anhydrase II. J Mol Biol 264:1101–1116
Article CAS PubMed Google Scholar
Williams RV et al (2022) AssignSLP_GUI, a software tool exploiting AI for NMR resonance assignment of sparsely labeled proteins. J Magn Reson 345:107336
Article CAS PubMed PubMed Central Google Scholar
Wu FJ, Kronenberg D, Hertel I, Grzesiek S (2023) The key role of glutamine for protein expression and isotopic labeling in insect cells. J Biol Chem 299:105142
Article CAS PubMed PubMed Central Google Scholar
Wüthrich K (2001) The way to NMR structures of proteins. Nat Struct Biol 8:923–925
Article CAS PubMed Google Scholar
Yamaguchi Y et al (2006) Glycoform-dependent conformational alteration of the fc region of human immunoglobulin G1 as revealed by NMR spectroscopy. Biochim Biophys Acta 1760:693–700
Article CAS PubMed Google Scholar
Yanaka S, Yagi H, Yogo R, Onitsuka M, Kato K (2022) Glutamine-free mammalian expression of recombinant glycoproteins with uniform isotope labeling: an application for NMR analysis of pharmaceutically relevant fc glycoforms of human immunoglobulin G1. J Biomol NMR 76:17–22
留言 (0)