Bone Marrow-Derived Mesenchymal Stem Cells (BM-MSCs)

Mesenchymal stem cells (MSCs) represent a type of adult stem cell endowed with self-renewal properties while being able to differentiate into diverse lineages [9]. These multipotent stem cells can be derived from a great degree of sources, including bone marrow, adipose tissues, synovial fluids, ligaments, muscle, umbilical cord, and placenta [10]. The employment of BM-MSCs has gained attention in the regenerative management of NGB. In in vivo models of NGB created by either complete or incomplete spinal cord injury (SCI), Salehi-pourmehr et al. [11] observed a notable improvement in urinary dysfunction subsequent to the administration of BM-MSCs sourced from rats directly into the bladder wall. Noteworthy, statistically significant changes in bladder compliance and residual volume were noticed in the hemi-SCI group at the study’s conclusion, albeit these metrics did not fully revert to pre-SCI levels. Furthermore, histological analysis showed a reduction in collagen deposition in groups treated with BM-MSCs. The BM-MSCs mitigated bladder dysfunction in the SCI model, notably in the hemi-SCI group, when transplanted during chronic injury phases. The transplantation of BM-MSCs into the bladder wall might present a novel therapeutic avenue for alleviating bladder dysfunction in SCI patients during the chronic phase of healing, particularly those afflicted with NGB disorder [11]. In a comparable study, Shen et al. [12] administered BM-MSCs into the bladder wall of rats subjected to bilateral pelvic nerve crush. Subsequently, a similar improvement in urodynamic parameters and a reduction in collagen deposition were observed. Western blot analysis showed that the injured rats, post-MSC treatment, exhibited a rise in α-SMA expression while manifesting a decline in TGF-β1, Smad2/3, and collagen type I and III expression in contrast to the control injured rats [12]. Given the association of the TGF-β1 pathway with tissue fibrosis [13], the treatment with BM-MSCs seems to exhibit anti-fibrotic attributes in the bladder. Additionally, the authors propose that BM-MSCs might foster nerve regeneration, as delineated by the enhanced expression of cholinergic neuron biomarkers in the MSC-treated animals. This study suggested that therapy with BM-MSCs could inhibit detrusor fibrosis, enhance intravesical pressure and voiding efficiency, and partially improve voiding function in rats subjected to bilateral pelvic nerve crush [12].

Immortalized Stem Cells (imMSCs)

Tian et al. [14] suggested that an environment rich in brain-derived neurotrophic factor (BDNF) increases the regenerative effects observed with stem cell therapy. Injecting imMSCs into the bladder wall led to functional improvements of the lower urinary tract post-bilateral major pelvic ganglion injury. However, MSCs engineered to exhibit high BDNF expression showed significantly superior improvements. In cohorts treated with BDNF overexpressing MSCs, the recovery of muscle and nerve tissue was expedited. The authors hypothesized that the BDNF/TrκB/CREB signaling pathway was essential in neural tissue regeneration, as MSCs expressing BDNF resulted in a marked rise in phosphorylation levels. In a rat model of NGB instigated by nerve injury, the impact of MSCs was evident on nerve tissue repair, contributing positively to functional recovery and tissue repair of the NGB. Concurrently, it was demonstrated that enhanced expression of BDNF, which holds a specific reparative effect on nerve injury, could more efficaciously repair the injured major pelvic ganglion in the local microenvironment. This mechanism might be correlated with the activation of the BDNF/TrκB/CREB signaling pathway and the mitigation of apoptosis by the highly expressed BDNF [14].

Placental Stem/Stromal Cells (PMSCs)

Yao et al. [15] developed a rat model of NGB deriving from SCI. On the ninth day post-injury, nerve cells derived from PMSCs were administered at the injury site. Subsequent urodynamic examination revealed partial restoration of bladder function, and an apparent improvement in detrusor muscle cell morphology was observed. The authors suggested that the post-injury recovery was improved by the MSCs’ capacity to differentiate into neurons, providing a protective barrier against apoptosis of cells, thereby aiding in maintaining bladder functionality. The findings of this study demonstrate that the transplantation of PMSCs could rapidly differentiate into nerve cells to compensate for the extensive apoptosis of cells [15].

Human Amniotic Fluid Stem Cells (hAFSCs)

Amniotic fluid stem cells have been explored for potential utilization in NGB therapy. In an in vivo model employing unilateral and bilateral pelvic nerve transection (PNT), Liang et al. [16] administered stem cells collected from amniotic fluid to the injury site. A cytometric assessment revealed improvements in pelvic nerve damage with hAFSCs. Moreover, both groups exhibited recovery of neurofilament density and PGP9.5 mRNA following the administration of hAFSCs. Expressions of GAP-43, p75, and NGF were diminished, while encephalins were elevated back to control levels after the treatment, indicating their involvement in this process. In summary, bladder dysfunction triggered by pelvic nerve transection could see improvement following hAFSC transplantation, with PGP9.5, GAP-43, neurotrophins, and encephalin potentially playing roles in nerve regeneration [16].

Human Umbilical Cord Mesenchymal Stem Cells (hUC-MSCs)

Human umbilical cord-derived mesenchymal stem cells (hUC-MSCs) facilitate revascularization, reduce inflammation, and enhance the microenvironment for SCI treatment [17•]. To decipher the mechanism underlying hUC-MSC-based NGB treatment, Li et al. [18] administered the cells to rats. Through Western blot and qPCR analyses, it was observed that following SCI, the levels of collagen type I/III, FGF2, p-p38, TRPV1, TLR4, and p-NF-κB increased. However, post-MSC treatment, the levels of the proteins mentioned above were significantly decreased. It was assumed that bladder function improvement was attributed to the inhibition of the p38 MAPK/NF-κB pathway by MSCs. Animals treated with MSCs exhibited increased neuronal cell survival alongside reduced edema, inflammatory cell presence, fibrosis, and collagen deposition. In conclusion, hUC-MSCs aid in restoring bladder function post-SCI by inhibiting the p38 MAPK/NF-κB pathway [18].

Adipose-Derived Stem Cells (ADSCs)

A study by Tien et al. [19] assessed the impact of autologous adipose-derived stem cell (ADSC) transplantation on acute SCI. Forty-seven SCI patients were divided into intervention and control groups, with ADSCs cultured and transplanted in the intervention group. Post-transplantation assessments revealed improvements in motor function, bladder function, and daily living activities for all patients, with no reported side effects. All patients experienced enhanced quality of life, with 80% expressing satisfaction with the treatment outcomes. The findings suggest that ADSC transplantation may be a safe and effective therapeutic approach for SCI patients while resulting in a significant improvement in muscle function [19].

Induced Pluripotent Stem Cells (iPSCs)

Being less differentiated compared to multipotent MSCs, pluripotent stem cells have been explored for similar applications in treating NGB. Shao et al. [20] employed an in vivo SCI-based NGB model to delve into the role of autophagy in treatment with neural stem cells derived from induced pluripotent stem cells (iPSCs). The study highlighted that the autophagy induction agent rapamycin facilitated the differentiation of iPSCs into neural stem cells by inhibiting the mTOR pathway. Additionally, upon implantation of autophagy-induced iPSCs, bladder tissue repair was noticed, while autophagy-inhibited cells yielded the reverse effect. These findings reveal that transplanting iPSC-derived neural stem cells (NSCs) can alleviate NGB following SCI, whereas autophagy inhibition results in exacerbated bladder tissue damage in SCI-affected rats, manifesting as bladder wall thickening and collagen deposition. These outcomes imply that autophagy acts as a self-repair, renewal, and protective mechanism in the transplantation of iPSC-derived NSCs for NGB treatment [20].

Stromal Cell-Derived Factor-1 (SDF-1) Mediated MSC Recruitment

SDF-1 is a highly potent chemoattractant signal involved in stem cell homing [21••]. Several studies propose that SDF-1 might play a role in the regenerative properties of BM-MSCs in treating NGB models. In a study by Zhu et al. [22••], rats with bilateral pelvic nerve damage were treated using upregulated and downregulated SDF-1 BM-MSCs. Notably, a significant improvement of NGB symptoms was observed in the groups treated with high SDF-1 MSCs, alongside enhancements in nerve repair and smooth muscle recovery. A high SDF-1 environment correlated with elevated expression levels of nNos, VEGF, and bFGF. The PI3K/AKT/mTOR and MAPK signaling pathways might be crucial to nerve recovery, as the upregulated and downregulated SDF-1 MSCs triggered their activity. Specifically, the high SDF-1 group exhibited the most explicit expression of mediators related to these pathways [22••]. In a study by Cho et al. [23], high SDF-1 expressing BM-MSCs were administered to rats subjected to bilateral pelvic nerve crush procedures. The researchers observed enhanced smooth muscle recovery associated with an upregulation of α-SMA expression in the bladder wall. Additionally, nerve recovery in the high SDF-1 group was increased, as evidenced by elevated beta-III-tubulin expression. Collectively, these investigations emphasized a promising association between SDF-1 and the tissue regenerative capacities of MSCs in the context of NGB. The authors suggest stem cell treatment may improve NGB function and recover atrophic bladder by promoting angiogenesis, tissue regeneration, and nerve recovery [23].

Neural Progenitor Cells (NPCs)

In a recent phase I clinical trial (NCT01933802), Harris et al. [24•] explored the treatment of multiple sclerosis utilizing NPCs derived from BM-MSCs. Out of the 20 participants enrolled in the trial, half exhibited enhanced bladder function following the administration of NPCs. Among the two participants diagnosed with NGB, both experienced alleviation of NGB symptoms following the treatment. Additionally, post-intrathecal NPC treatment, 70% and 50% of the participants exhibited enhanced muscle strength and bladder function, respectively. The observed potential reversal of disability in a subset of patients advocates for a larger phase II placebo-controlled study to ascertain the efficacy of intrathecal NPC treatment in individuals with multiple sclerosis [24•]. A summary of included studies in this review can be found in Table 1 and Fig. 1. Figure 2 indicates the remodeling process of NGB mediated by inflammation and growth factors.

Table 1 Key findings of the studies are included in this reviewFig. 1

An overview of selected articles in the review paper

Fig. 2

A summary of neurogenic bladder remodeling process driven by inflammation and growth factors