Abdali N, Parks JM, Haynes KM, Chaney JL, Green AT, Wolloscheck D, Walker JK, Rybenkov VV, Baudry J, Smith JC, Zgurskaya HI (2017) Reviving antibiotics: efflux pump inhibitors that interact with AcrA, a membrane fusion protein of the AcrAB-TolC multidrug efflux pump. ACS Infect Dis 3:89–98

Article  CAS  PubMed  Google Scholar 

Aboelenin AM, Hassan R, Abdelmegeed ES (2021) The effect of EDTA in combination with some antibiotics against clinical isolates of gram negative bacteria in Mansoura. Egypt Microb Pathog 154:104840

Article  CAS  PubMed  Google Scholar 

Andini S, Araya-Cloutier C, Lay B, Vreeke G, Hageman J, Vincken JP (2021) QSAR-based physicochemical properties of isothiocyanate antimicrobials against gram-negative and gram-positive bacteria. Lwt-Food Science and Technology 144:111222

Article  CAS  Google Scholar 

Annunziato G (2019) Strategies to overcome antimicrobial resistance (AMR) making use of non-essential target inhibitors: a review. Int J Mol Sci 20:5844

Article  CAS  PubMed  PubMed Central  Google Scholar 

Borges A, Abreu AC, Ferreira C, Saavedra MJ, Simoes LC, Simoes M (2015) Antibacterial activity and mode of action of selected glucosinolate hydrolysis products against bacterial pathogens. J Food Sci Technol 52:4737–4748

Article  CAS  PubMed  Google Scholar 

Bowler P, Murphy C, Wolcott R (2020) Biofilm exacerbates antibiotic resistance: is this a current oversight in antimicrobial stewardship? Antimicrob Resist Infect Control 9:162

Article  PubMed  PubMed Central  Google Scholar 

Clifton LA, Skoda MW, Le Brun AP, Ciesielski F, Kuzmenko I, Holt SA, Lakey JH (2015) Effect of divalent cation removal on the structure of gram-negative bacterial outer membrane models. Langmuir 31:404–412

Article  CAS  PubMed  Google Scholar 

CLSI (2019) CLSI (2019) CLSI document M100 – performance standards for antimicrobial susceptibility testing, 29th edn. CLSI, Wayne, PA

Google Scholar 

Cochran JW, Byrne RW (1974) Isolation and properties of a ribosome-bound factor required for ppGpp and pppGpp synthesis in Escherichia coli. J Biol Chem 249:353–360

Article  CAS  PubMed  Google Scholar 

Grassi L, Di Luca M, Maisetta G, Rinaldi AC, Esin S, Trampuz A, Batoni G (2017) Generation of persister cells of Pseudomonas aeruginosa and Staphylococcus aureus by chemical treatment and evaluation of their susceptibility to membrane-targeting agents. Front Microbiol 8:1917

Article  PubMed  PubMed Central  Google Scholar 

Hancock RE, Wong PG (1984) Compounds which increase the permeability of the Pseudomonas aeruginosa outer membrane. Antimicrob Agents Chemother 26:48–52

Article  CAS  PubMed  PubMed Central  Google Scholar 

Hauryliuk V, Atkinson GC, Murakami KS, Tenson T, Gerdes K (2015) Recent functional insights into the role of (p)ppGpp in bacterial physiology. Nat Rev Microbiol 13:298–309

Article  CAS  PubMed  PubMed Central  Google Scholar 

Impey RE, Hawkins DA, Sutton JM, Soares da Costa TP (2020) Overcoming intrinsic and acquired resistance mechanisms associated with the cell wall of Gram-negative bacteria. Antibiotics (basel) 9:623

Article  CAS  PubMed  Google Scholar 

Jang M, Hong E, Kim GH (2010) Evaluation of antibacterial activity of 3-butenyl, 4-pentenyl, 2-phenylethyl, and benzyl isothiocyanate in Brassica vegetables. J Food Sci 75:M412-416

Article  CAS  PubMed  Google Scholar 

Kaushik V, Sharma S, Tiwari M, Tiwari V (2022) Antipersister strategies against stress induced bacterial persistence. Microb Pathog 164:105423

Article  CAS  PubMed  Google Scholar 

Kim MG, Lee HS (2009) Growth-inhibiting activities of phenethyl isothiocyanate and its derivatives against intestinal bacteria. J Food Sci 74:M467-471

Article  CAS  PubMed  Google Scholar 

Kim HC, Jang TW, Chae HJ, Choi WJ, Ha MN, Ye BJ, Kim BG, Jeon MJ, Kim SY, Hong YS (2015) Evaluation and management of lead exposure. Ann Occup Environ Med 27:30

Article  PubMed  PubMed Central  Google Scholar 

Ko MO, Kim MB, Lim SB (2016) Relationship between chemical structure and antimicrobial activities of isothiocyanates from cruciferous vegetables against oral pathogens. J Microbiol Biotechnol 26:2036–2042

Article  CAS  PubMed  Google Scholar 

Krishnamoorthy G, Leus IV, Weeks JW, Wolloscheck D, Rybenkov VV, Zgurskaya HI (2017) Synergy between active efflux and outer membrane diffusion defines rules of antibiotic permeation into Gram-negative bacteria. MBio 8:e01172-01117

Article  CAS  PubMed  PubMed Central  Google Scholar 

Kwan BW, Valenta JA, Benedik MJ, Wood TK (2013) Arrested protein synthesis increases persister-like cell formation. Antimicrob Agents Chemother 57:1468–1473

Article  CAS  PubMed  PubMed Central  Google Scholar 

Lanigan RS, Yamarik TA (2002) Final report on the safety assessment of EDTA, calcium disodium EDTA, diammonium EDTA, dipotassium EDTA, disodium EDTA, TEA-EDTA, tetrasodium EDTA, tripotassium EDTA, trisodium EDTA, HEDTA, and trisodium HEDTA. Int J Toxicol 21(Suppl 2):95–142

CAS  PubMed  Google Scholar 

Li XZ, Plesiat P, Nikaido H (2015) The challenge of efflux-mediated antibiotic resistance in Gram-negative bacteria. Clin Microbiol Rev 28:337–418

Article  PubMed  PubMed Central  Google Scholar 

Menegucci TC, Fedrigo NH, Lodi FG, Albiero J, Nishiyama SAB, Mazucheli J, Carrara-Marroni FE, Voelkner NMF, Gong H, Sy SKB, Tognim MCB (2019) Pharmacodynamic effects of sulbactam/meropenem/polymyxin-B combination against extremely drug resistant Acinetobacter baumannii using checkerboard information. Microb Drug Resist 25:1266–1274

Article  CAS  PubMed  Google Scholar 

Narayanaswamy VP, Keagy LL, Duris K, Wiesmann W, Loughran AJ, Townsend SM, Baker S (2018) Novel glycopolymer eradicates antibiotic- and CCCP-induced persister cells in Pseudomonas aeruginosa. Front Microbiol 9:1724

Article  PubMed  PubMed Central  Google Scholar 

Ni W, Li Y, Guan J, Zhao J, Cui J, Wang R, Liu Y (2016) Effects of efflux pump inhibitors on colistin resistance in multidrug-resistant Gram-negative bacteria. Antimicrob Agents Chemother 60:3215–3218

Article  CAS  PubMed  PubMed Central  Google Scholar 

Nowicki D, Maciag-Dorszynska M, Bogucka K, Szalewska-Palasz A, Herman-Antosiewicz A (2019) Various modes of action of dietary phytochemicals, sulforaphane and phenethyl isothiocyanate, on pathogenic bacteria. Sci Rep 9:13677

Article  ADS  PubMed  PubMed Central  Google Scholar 

Nowicki D, Krause K, Szamborska P, Zukowska A, Cech GM, Szalewska-Palasz A (2020) Induction of the stringent response underlies the antimicrobial action of aliphatic isothiocyanates. Front Microbiol 11:591802

Article  PubMed  Google Scholar 

Osei Sekyere J, Amoako DG (2017) Carbonyl cyanide m-chlorophenylhydrazine (CCCP) reverses resistance to colistin, but not to carbapenems and tigecycline in multidrug-resistant Enterobacteriaceae. Front Microbiol 8:228

Article  PubMed  PubMed Central  Google Scholar 

Pancu DF, Scurtu A, Macasoi IG, Marti D, Mioc M, Soica C, Coricovac D, Horhat D, Poenaru M, Dehelean C (2021) Antibiotics: Conventional therapy and natural compounds with antibacterial activity-a pharmaco-toxicological screening. Antibiotics (Basel) 10:401

Article  CAS  PubMed  Google Scholar 

Pausch P, Abdelshahid M, Steinchen W, Schafer H, Gratani FL, Freibert SA, Wolz C, Turgay K, Wilson DN, Bange G (2020) Structural basis for regulation of the opposing (p)ppGpp synthetase and hydrolase within the stringent response orchestrator Rel. Cell Rep 32:108157

Article  CAS  PubMed  Google Scholar 

Pelletier C, Bourlioux P, van Heijenoort J (1994) Effects of sub-minimal inhibitory concentrations of EDTA on growth of Escherichia coli and the release of lipopolysaccharide. FEMS Microbiol Lett 117:203–206

Article  CAS  PubMed  Google Scholar 

Romeo L, Iori R, Rollin P, Bramanti P, Mazzon E (2018) Isothiocyanates: an overview of their antimicrobial activity against human infections. Molecules 23:624

Article  PubMed  PubMed Central  Google Scholar 

Salehi B, Ghalavand Z, Yadegar A, Eslami G (2021) Characteristics and diversity of mutations in regulatory genes of resistance-nodulation-cell division efflux pumps in association with drug-resistant clinical isolates of Acinetobacter baumannii. Antimicrob Resist Infect Control 10:53

Article  PubMed  PubMed Central  Google Scholar 

Schrank CL, Wilt IK, Monteagudo Ortiz C, Haney BA, Wuest WM (2021) Using membrane perturbing small molecules to target chronic persistent infections. RSC Med Chem 12:1312–1324

Article  CAS  PubMed  PubMed Central  Google Scholar 

Shin J, Prabhakaran VS, Kim KS (2018) The multi-faceted potential of plant-derived metabolites as antimicrobial agents against multidrug-resistant pathogens. Microb Pathog 116:209–214

Article  CAS  PubMed  Google Scholar 

Sim JXF, Khazandi M, Pi H, Venter