Bellè F, Mercatanti A, Lodovichi S, Congregati C, Guglielmi C, Tancredi M, Caligo MA, Cervelli T, Galli A (2022) Validation and data-integration of yeast-based assays for functional classification of BRCA1 missense variants. Int J Mol Sci. https://doi.org/10.3390/ijms23074049

Article  PubMed  PubMed Central  Google Scholar 

Botstein D, Chervitz SA, Cherry JM (1997) Yeast as a model organism. Science. https://doi.org/10.1126/science.277.5330.1259

Article  PubMed  PubMed Central  Google Scholar 

Cannan WJ, Pederson DS (2016) Mechanisms and consequences of double-strand DNA break formation in chromatin. J Cell Physiol 231(1):3–14. https://doi.org/10.1002/jcp.25048

Article  CAS  PubMed  PubMed Central  Google Scholar 

Cervelli T, Lodovichi S, Bellè F, Galli A (2020) Yeast-based assays for the functional characterization of cancer-associated variants of human DNA repair genes. Microb Cell. https://doi.org/10.15698/mic2020.07.721

Article  PubMed  PubMed Central  Google Scholar 

Chatterjee N, Walker GC (2017) Mechanisms of DNA damage, repair, and mutagenesis. Environ Mol Mutagen. https://doi.org/10.1002/em.22087

Article  PubMed  PubMed Central  Google Scholar 

Clear AD, Manthey GM, Lewis O, Lopez IY, Rico R, Owens S, Negritto MC, Wolf EW, Xu J, Kenjić N, Perry JJP, Adamson AW, Neuhausen SL, Bailis AM (2020) Variants of the human RAD52 gene confer defects in ionizing radiation resistance and homologous recombination repair in budding yeast. Microb Cell. https://doi.org/10.15698/mic2020.10.732

Article  PubMed  PubMed Central  Google Scholar 

Costanza A, Guaragnella N, Bobba A, Manzari C, L’Abbate A, Giudice CL, Picardi E, D’Erchia AM, Pesole G, Giannattasio S (2022) Yeast as a model to unravel new BRCA2 functions in cell metabolism. Front Oncol. https://doi.org/10.3389/fonc.2022.908442,12,908442

Article  PubMed  PubMed Central  Google Scholar 

Danilowicz C, Vietorisz E, Godoy-Carter V, Prévost C, Prentiss M (2021) Influences of ssDNA-RecA filament length on the fidelity of homologous recombination. J Mol Biol. https://doi.org/10.1016/j.jmb.2021.167143

Article  PubMed  Google Scholar 

Erdeniz N, Mortensen UH, Rothstein R (1997) Cloning-free PCR-based allele replacement methods. Genome Res. https://doi.org/10.1101/gr.7.12.1174

Article  PubMed  PubMed Central  Google Scholar 

Gonzaga-Jauregui C, Lupski JR, Gibbs RA (2012) Human genome sequencing in health and disease. Annu Rev Med 63:35–61. https://doi.org/10.1146/annurev-med-051010-162644

Article  CAS  PubMed  PubMed Central  Google Scholar 

Ho CK, Mazón G, Lam AF, Symington LS (2010) Mus81 and Yen1 promote reciprocal exchange during mitotic recombination to maintain genome integrity in budding yeast. Mol Cell. https://doi.org/10.1016/j.molcel.2010.11.016

Article  PubMed  PubMed Central  Google Scholar 

Hoffman-Andrews L (2017) The known unknown: the challenges of genetic variants of uncertain significance in clinical practice. J Law Biosci. https://doi.org/10.1093/jlb/lsx038

Article  PubMed  Google Scholar 

Holloman WK (2013) Unraveling the mechanism of BRCA2 in homologous recombination. Nat Struct Mol Biol. https://doi.org/10.1038/nsmb.2096

Article  Google Scholar 

Huang R, Zhou PK (2021) DNA damage repair: Historical perspectives, mechanistic pathways and clinical translation for targeted cancer therapy. Signal Transduct Target Ther. https://doi.org/10.1038/s41392-021-00648-7

Article  PubMed  PubMed Central  Google Scholar 

Hunter N, Kleckner N (2001) The single-end invasion: an asymmetric intermediate at the double-strand break to double-holliday junction transition of meiotic recombination. Cell. https://doi.org/10.1016/S0092-8674(01)00430-5

Article  PubMed  Google Scholar 

Huszno J, Pigłowski W, Mazur M, Pamuła-Piłat J, Zajkowicz A, Kierzkowska AF, Wojciechowska MO (2021) BRCA1/BRCA2 variants of uncertain significance in clinical practice: a case report. Mol Clin Oncol. https://doi.org/10.3892/mco.2021.2385

Article  PubMed  PubMed Central  Google Scholar 

Ishii A, Kurosawa A, Saito S, Adachi N (2014) Analysis of the role of homology arms in gene-targeting vectors in human cells. PLoS One 9(9): e108236. https://doi.org/10.1371/journal.pone.0108236

Article  CAS  PubMed  PubMed Central  Google Scholar 

Jensen RB, Carreira A, Kowalczykowski SC (2010) Purified human BRCA2 stimulates RAD51-mediated recombination. Nature. https://doi.org/10.1038/nature09399

Article  PubMed  PubMed Central  Google Scholar 

Klovstad M, Abdu U, Schüpbach T (2008) Drosophila brca2 is required for mitotic and meiotic DNA repair and efficient activation of the meiotic recombination checkpoint. PLoS Genet. https://doi.org/10.1371/journal.pgen.0040031

Article  PubMed  PubMed Central  Google Scholar 

Kuznetsov SG, Liu P, Sharan SK (2008) Mouse embryonic stem cell-based functional assay to evaluate mutations in BRCA2. Nat Med. https://doi.org/10.1038/nm.1719

Article  PubMed  PubMed Central  Google Scholar 

Kuznetsov SG, Chang S, Sharan SK (2010) Functional analysis of human BRCA2 variants using a mouse embryonic stem cell-based assay. Methods Mol Biol. https://doi.org/10.1007/978-1-60761-759-4_16

Article  PubMed  PubMed Central  Google Scholar 

Liu J, Doty T, Gibson B, Heyer WD (2010) Human BRCA2 protein promotes RAD51 filament formation on RPA-covered single-stranded DNA. Nat Struct Mol Biol 17(10):1260–1262. https://doi.org/10.1038/nsmb.1904

Article  CAS  PubMed  PubMed Central  Google Scholar 

Liu C, Wu P, Zhang A, Mao X (2021) Advances in rodent models for breast cancer formation, progression, and therapeutic testing. Front Oncol. https://doi.org/10.3389/fonc.2021.593337

Article  PubMed  PubMed Central  Google Scholar 

Lodovichi S, Bellè F, Mercatanti A, Spugnesi L, Cozzani C, Caligo MA, Cervelli T, Galli A (2022) RAD52 influences the effect of BRCA1/2 missense variants on homologous recombination and gene reversion in Saccharomyces cerevisiae. FEMS Yeast Res 22(1):foac021. https://doi.org/10.1093/femsyr/foac021

Article  CAS  PubMed  Google Scholar 

Manthey GM, Bailis AM (2010) RAD51 inhibits translocation formation by non-conservative homologous recombination in Saccharomyces cerevisiae. PLoS One. https://doi.org/10.1371/journal.pone.0011889

Article  PubMed  PubMed Central  Google Scholar 

Manthey GM, Clear AD, Liddell LC, Negritto MC, Bailis AM (2017) Homologous recombination in budding yeast expressing the human RAD52 gene reveals a Rad51-independent mechanism of conservative double-strand break repair. Nucleic Acids Res. https://doi.org/10.1093/nar/gkw1228

Article  PubMed  Google Scholar 

McCulloch RD, Read LR, Baker MD (2003) Strand invasion and DNA synthesis from the two 3′ ends of a double-strand break in Mammalian cells. Genetics. https://doi.org/10.1093/genetics/163.4.1439

Article  PubMed  PubMed Central  Google Scholar 

Nickoloff JA, Haber JE (2001) Mating-type control of DNA repair and recombination in Saccharomyces cerevisiae. Contemp Cancer Res. https://doi.org/10.1007/978-1-59259-095-7_5

Article  Google Scholar 

Nogueira A, Fernandes M, Catarino R, Medeiros R (2019) Rad52 functions in homologous recombination and its importance on genomic integrity maintenance and cancer therapy. Cancers. https://doi.org/10.3390/cancers11111622

Article  PubMed  PubMed Central  Google Scholar 

Petrucelli N, Daly MB, Feldman GL (2010) Hereditary breast and ovarian cancer due to mutations in BRCA1 and BRCA2. Genet Med. https://doi.org/10.1097/GIM.0b013e3181d38f2f

Article  PubMed  Google Scholar 

Roy R, Chun J, Powell SN (2011) BRCA1 and BRCA2: different roles in a common pathway of genome protection. Nat Rev Cancer. https://doi.org/10.1038/nrc3181

Article  PubMed  PubMed Central  Google Scholar 

San Filippo J, Sung P, Klein H (2008) Mechanism of eukaryotic homologous recombination. Annu Rev Biochem. https://doi.org/10.1146/annurev.biochem.77.061306.125255

Article  PubMed  Google Scholar 

Shinohara A, Ogawa T (1998) Stimulation by Rad52 of yeast Rad51-mediated recombination. Nature. https://doi.org/10.1038/34943

Article  PubMed  Google Scholar 

Spugnesi L, Balia C, Collavoli A, Falaschi E, Quercioli V, Caligo MA, Galli A (2013) Effect of the expression of BRCA2 on spontaneous homologous recombination and DNA damage-induced nuclear foci in Saccharomyces cerevisiae. Mutagenesis. https://doi.org/10.1093/mutage/ges069

Article  PubMed  Google Scholar 

Vierstraete J, Willaert A, Vermassen P, Coucke PJ, Vral A, Claes KBM (2017) Accurate quantification of homologous recombination in zebrafish: Brca2 deficiency as a paradigm. Sci Rep. https://doi.org/10.1038/s41598-017-16725-3

Article  PubMed  PubMed Central