1 Interdisciplinary Nanoscience Center, Aarhus University, Aarhus, Denmark.

2 Department of Physics, University of York, York, UK.

3 Department of Biochemistry and Molecular Biology, University of Southern Denmark, Odense, Denmark.

4 Department of Biology, University of York, York, UK.

5 Department of Biology, Aarhus University, Aarhus, Denmark.

# Joint first authors.

Keywords: fusion protein, Gram positive bacteria, monomeric superfolder GFP, coagulase, biofilms.

Abbreviations:

Coa – coagulase,

GFP – green fluorescent protein,

msfGFP – monomeric superfolder GFP,

Sec – Secretory pathway,

Tat – Twin Arginine Translocation pathway,

vWbp – von Willebrand factor binding protein.
Received originally: 22/12/2022 Received in revised form: 14/06/2023
Accepted: 19/06/2023 Published: 28/06/2023

Correspondence:
Rikke L. Meyer, Interdisciplinary Nanoscience Center, Aarhus University, Gustav Wieds Vej 14, 8000 Aarhus C, Denmark; rikke.meyer@inano.au.dk
Mark C. Leake, Departments of Physics and Biology, University of York, York, YO10 5DD, UK; mark.leake@york.ac.uk

Conflict of interest statement: The authors declare no conflicts of interest.
Please cite this article as: Dominique C. S. Evans, Amanda B. Khamas, Lisbeth Marcussen, Kristian S. Rasmussen, Janne K. Klitgaard, Birgitte H. Kallipolitis, Janni Nielsen, Daniel E. Otzen, Mark C. Leake and Rikke L. Meyer (2023). GFP fusions of Sec-routed extracellular proteins in Staphylococcus aureus reveal surface-associated coagulase in biofilms. Microbial Cell 10(7): 145-156. doi: 10.15698/mic2023.07.800