Document Type : Research Paper

Authors

1 Brain and Spinal Cord Injury Research Center, Neuroscience Institute, Tehran University of Medical Science, Tehran, Iran

2 Department of Pathology, School of Veterinary Medicine, Sharekord University, Shahrekord, Iran

3 department of tissue engineering and applied cell sciences , school of advanced technologies , Tehran university of medical sciences , Tehran , Iran

4 Department of Periodontics, Dental Research Center, School of Dentistry, Hamadan University of Medical Sciences, Hamadan,Iran

5 Department of Immunology, School of medicine, Tehran University of Medical Sciences, Tehran, Iran.

6 Department of Medical Biotechnology School of Advanced Technologies in Medical, Tehran university of Medical Sciences

7 Department of Animal Biology, School of Biology, College of Science, University of Tehran, Tehran, Iran

8 Department of Tissue Engineering, TUMS, Tehran, Iran

10.30498/ijb.2023.362229.3505

Abstract

Background: Mesenchymal Stem Cell (MSC) Derived Exosomes (Mscs-DE) Have Been Demonstrated to Be Potential Candidates for Treatment of Rat Spinal Cord Injury (SCI).

Objective: The Effect of AD-Mscs and AD-MSC-DE Encapsulated into Collagen and Fibrin Hydrogels On Treatment of SCI in A Rat Animal Model Was Aimed.

Materials and Methods: Fibrin and Collagen Hydrogels Were Synthesized and Then Mixed with AD-Mscs Suspension. The AD-MSC-Des Were Isolated Using Ultra-Centrifugation at 100,000×g for 120 Min and Were Characterized. The Characterized AD-MSC-Des Were Encapsulated into Collagen and Fibrin Hydrogels. Eighteen Adult Male Wistar Rats Were Randomly Classified into 3 Equal Groups (N=6): The Control Group (SCI Rat Without Treatment), SCI Rat Treated with Either AD-MSC-DE Encapsulated in Collagen Hydrogel or Encapsulated in Fibrin Hydrogel Groups. The Treatment Approaches Were Evaluated Using Clinical, Histological and Molecular Assays.

Results: The AD-MSC-DE Encapsulated into Fibrin and Collagen Groups Showed Better Clinical Function Than the Control Group. The AD-MSC-DE Encapsulated into Fibrin and Collagen Also Improved SCI-Induced Polio and Leuko-Myelomalacia and Led to Higher Expression NF Protein Than the Control Group. In The AD-MSC-DE Encapsulated into Collagen and Fibrin Led to Up-Regulation The Mean Levels of NEFL (23.82 And 24.33, Respectively), Enos (24.31 And 24.53, Respectively), And CK19 mRNAs (24.23 And 23.98, Respectively) Compared to The Control Group.

Conclusion: The AD-Mscs-DE Encapsulated Within ECM-Based Hydrogel Scaffolds Such as Collagen and Fibrin Can Regenerate the Injured Nerve in SCI Rat and Reduce Spinal Cord Lesion-Induced Central Neuropathic Pain.

Key Word: Mesenchymal Stem Cell Derived Exosomes, Fibrin, Collagen, Hydrogel, Spinal Cord Injury

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