Available online 23 November 2022, 115864
Author links open overlay panelABSTRACTA rapid (<3h) and reliable multiplex PCR was developed for detecting simultaneously known plasmid-mediated fos genes conferring acquired resistance to fosfomycin. Our technique was tested on a collection of Escherichia coli isolates previously identified as bearing the fosA-, fosC- and fosL-like genes, showing a sensitivity and a specificity of 100%.
Section snippetsCRediT authorship contribution statementSamanta Freire: Methodology, Investigation. Teresa Grilo: Methodology, Investigation. Patrice Nordmann: Writing – review & editing. Laurent Poirel: Conceptualization, Methodology, Investigation, Writing – review & editing. Marta Aires-de-Sousa: Conceptualization, Methodology, Investigation, Supervision, Writing – original draft, Writing – review & editing.
Declaration of competing interestThis study was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest. All the authors declare the absence of any dual or conflicting interest.
FundingThis work was partially supported by the Portuguese Red Cross, by the University of Fribourg, and by the Swiss National Science Foundation (projects FNS-31003A_163432 and FNS-407240_177381).
Ethical approvalNot required.
REFERENCES (22)TY Yang et al.Update on fosfomycin-modified genes in EnterobacteriaceaeJ Microbiol Immunol Infect
(2019)
WE Stamm et al.Urinary tract infections: disease panorama and challengesJ Infect Dis
(2001)
AL Flores-Mireles et al.Urinary tract infections: epidemiology, mechanisms of infection and treatment optionsNat Rev Microbiol
(2015)
ME Falagas et al.FosfomycinClin Microbiol Rev
(2016)
LL. SilverFosfomycin: mechanism and resistanceCold Spring Harb Perspect Med
(2017)
K Zurfluh et al.Fosfomycin resistance genes in Enterobacteriaceae-An increasing threatMicrobiologyopen
(2020)
R Ito et al.Widespread fosfomycin resistance in gram-negative bacteria attributable to the chromosomal fosA genemBio
(2017)
H Xu et al.Identification of a novel fosfomycin resistance gene (fosA2) in Enterobacter cloacae from the Salmon RiverCanada. Lett Appl Microbiol
(2011)
MA Rehman et al.First Detection of a fosfomycin resistance Gene, fosA7, in Salmonella enterica serovar Heidelberg isolated from broiler chickensAntimicrob Agents Chemother
(2017)
R Ito et al.Origin of the plasmid-mediated fosfomycin resistance gene fosA3J Antimicrob Chemother
(2018)
MM Rodriguez et al.Proposing Kluyvera georgiana as the origin of the plasmid-mediated resistance gene fosA4Antimicrob Agents Chemother
(2018)
View full text© 2022 Elsevier Inc. All rights reserved.
留言 (0)