Leptospirosis poses a significant threat as a reemerging disease, leading to acute febrile illness in many developing countries [1].

The clinical manifestations of leptospirosis are diverse and complex, and infected humans may present minimal or no clinical symptoms. Due to its non-specific symptoms and restricted diagnostic methods, leptospirosis is often misdiagnosed as influenza, aseptic meningitis, hepatic disease, or other infectious diseases, such as COVID-19 [2]. Thus, early detection,