Protocol-dependent morphological changes in human embryonic stem cell aggregates during differentiation towards early pancreatic fate

Stem Cells / Research Article

Rezaei Zonooz E. · Ghezelayagh Z. · Moradmand A. · Baharvand H. · Tahamtani Y.

Log in to MyKarger to check if you already have access to this content.

Buy FullText & PDF Unlimited re-access via MyKarger Unrestricted printing, no saving restrictions for personal use
read more

CHF 38.00 *
EUR 35.00 *
USD 39.00 *

Select

KAB

Buy a Karger Article Bundle (KAB) and profit from a discount!

If you would like to redeem your KAB credit, please log in.

Save over 20% compared to the individual article price.

Learn more

Access via DeepDyve Unlimited fulltext viewing Of this article Organize, annotate And mark up articles Printing And downloading restrictions apply

Select

Subscribe Access to all articles of the subscribed year(s) guaranteed for 5 years Unlimited re-access via Subscriber Login or MyKarger Unrestricted printing, no saving restrictions for personal use read more

Subcription rates

Select

* The final prices may differ from the prices shown due to specifics of VAT rules.

Article / Publication Details Abstract

Cell therapy is one of the promising approaches used against type1 diabetes. Efficient generation of Human embryonic stem cell (hESC)-derived pancreatic progenitors (PPs) is of great importance. Since signaling pathways underlying human pancreas development is not yet fully understood, various differentiation protocols are conducted each considering variable duration, timing and concentrations of growth factors and small molecules. Therefore, we compared two PP differentiation protocols in static suspension culture. We tested modified protocols developed by Pagliuca et al. (protocol-1) and Royan researchers (protocol-2) until early PP stage. The morphological changes of hESC aggregates during differentiation, and also gene and protein expression after differentiation were evaluated. Different morphological structures were formed in each protocol. Quantitative gene expression analysis, flow cytometry and immunostaining revealed a high level of PDX1 expression on day 13 of Royan’s differentiation protocol compared to protocol-1. Our data showed that using protocol-2, cells were further differentiated until day16 showing higher efficiency of early PPs. Moreover, protocol-2 is able to produce hESCs-PPs in a static suspension culture. Since protocol-2 is inexpensive in terms of media, growth factors and chemicals, it can be used for massive production of PPs using static and dynamic suspension cultures.

S. Karger AG, Basel

Article / Publication Details Copyright / Drug Dosage / Disclaimer Copyright: All rights reserved. No part of this publication may be translated into other languages, reproduced or utilized in any form or by any means, electronic or mechanical, including photocopying, recording, microcopying, or by any information storage and retrieval system, without permission in writing from the publisher.
Drug Dosage: The authors and the publisher have exerted every effort to ensure that drug selection and dosage set forth in this text are in accord with current recommendations and practice at the time of publication. However, in view of ongoing research, changes in government regulations, and the constant flow of information relating to drug therapy and drug reactions, the reader is urged to check the package insert for each drug for any changes in indications and dosage and for added warnings and precautions. This is particularly important when the recommended agent is a new and/or infrequently employed drug.
Disclaimer: The statements, opinions and data contained in this publication are solely those of the individual authors and contributors and not of the publishers and the editor(s). The appearance of advertisements or/and product references in the publication is not a warranty, endorsement, or approval of the products or services advertised or of their effectiveness, quality or safety. The publisher and the editor(s) disclaim responsibility for any injury to persons or property resulting from any ideas, methods, instructions or products referred to in the content or advertisements.

留言 (0)

沒有登入
gif