Gomaa, M., Sheta, E. (2022). Efficacy of Cuminum cyminum L. Seed Oil on Acute Toxoplasmosis: An Experimental Study on Albino Mice.. Parasitologists United Journal, (), -. doi: 10.21608/puj.2022.124104.1158

Maha Mohamed Gomaa; Eman Sheta. "Efficacy of Cuminum cyminum L. Seed Oil on Acute Toxoplasmosis: An Experimental Study on Albino Mice.". Parasitologists United Journal, , , 2022, -. doi: 10.21608/puj.2022.124104.1158

Gomaa, M., Sheta, E. (2022). 'Efficacy of Cuminum cyminum L. Seed Oil on Acute Toxoplasmosis: An Experimental Study on Albino Mice.', Parasitologists United Journal, (), pp. -. doi: 10.21608/puj.2022.124104.1158

Gomaa, M., Sheta, E. Efficacy of Cuminum cyminum L. Seed Oil on Acute Toxoplasmosis: An Experimental Study on Albino Mice.. Parasitologists United Journal, 2022; (): -. doi: 10.21608/puj.2022.124104.1158

Articles in Press, Accepted Manuscript, Available Online from 07 April 2022   Document Type: Original Article DOI: 10.21608/puj.2022.124104.1158Authors Maha Mohamed Gomaa 1; Eman Sheta212 Ahmed Hamdy Street from Abdel Salam Aref street, Louran2Pathology Department, Alexandria Faculty of Medicine, Egypt.AbstractToxoplasmosis is one of the most common parasitic infections affecting human and warm-blooded animals, caused by Toxoplasma gondii. T. gondii is also a highly significant economic and veterinary medical concern in livestock industry as an important zoonosis. Although the available therapeutics possess adequate efficiencies, they are associated with adverse effects. This highlights the urgent need for development of new, safe and cost-effective medications for treatment of toxoplasmosis. Natural compounds are the most productive source for the treatment of a wide range of diseases. Herein, we assessed the efficacy of cumin seed oil (CSO), for the first time in the treatment of acute toxoplasmosis in mice, experimentally-infected with virulent T. gondii RH strain in comparison to a reference drug SeptrinTM. It is the essential oil of the spice “Cuminum cyminum L”. The total phenol and flavonoid contents of CSO were determined to be 84.65 ± 0.22 mg GAE/g and 52.08 ± 0.18 mg QE/g, respectively. It was orally administered at a dose of 5mL/Kg/day for five consecutive days. Both SeptrinTM and CSO had markedly improved the animal survival and reduced the parasite burdens in liver, spleen, brain and peritoneal exudate. They significantly deteriorated tachyzoites viability and infectivity. In CSO-treated mice, distorted crescent-shaped tachyzoites, deep depressions, membrane pores and huge vesicular swellings were detected by scanning electron microscopy. On immunological basis, treatment with either SeptrinTM or CSO obviously decreased tumor necrosis factor alpha (TNF-α)-serum-level. It substantially reduced the necro-inflammatory reaction and ameliorated the histopathologic changes in different organs. the evidences gathered herein, support that CSO may be useful as a safe, natural therapy for acute toxoplasmosis considering both the anti-inflammatory and the anti-Toxoplasma properties. However, the mode of antiprotozoal action of the oil components should be further investigated.Keywords Toxoplasma gondii RH strain; Cumin seed oil; Swiss Albino mice; Tumor necrosis factor alpha (TNF-α); Scanning electron microscopy; Hepatic necro-inflammatory grading; Brain histopathology Main Subjects Protozoology

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