Abdel Menaem, H., Mostafa, M., Sarhan, R., William, S., Abdel Rahman, A. (2022). Experimental in vivo assessment of immunomodulatory effect of Kalobin (Pelargonium reinforme/sidoides extract) on schistosomiasis mansoni. Parasitologists United Journal, (), -. doi: 10.21608/puj.2022.100824.1138

Heba Abdel Menaem; Manal Mostafa; Rania Sarhan; Samia William; Abeer Abdel Rahman. "Experimental in vivo assessment of immunomodulatory effect of Kalobin (Pelargonium reinforme/sidoides extract) on schistosomiasis mansoni". Parasitologists United Journal, , , 2022, -. doi: 10.21608/puj.2022.100824.1138

Abdel Menaem, H., Mostafa, M., Sarhan, R., William, S., Abdel Rahman, A. (2022). 'Experimental in vivo assessment of immunomodulatory effect of Kalobin (Pelargonium reinforme/sidoides extract) on schistosomiasis mansoni', Parasitologists United Journal, (), pp. -. doi: 10.21608/puj.2022.100824.1138

Abdel Menaem, H., Mostafa, M., Sarhan, R., William, S., Abdel Rahman, A. Experimental in vivo assessment of immunomodulatory effect of Kalobin (Pelargonium reinforme/sidoides extract) on schistosomiasis mansoni. Parasitologists United Journal, 2022; (): -. doi: 10.21608/puj.2022.100824.1138

Articles in Press, Accepted Manuscript, Available Online from 29 March 2022   Document Type: Original Article DOI: 10.21608/puj.2022.100824.1138Authors Heba Abdel Menaem1; Manal Mostafa2; Rania Sarhan 3; Samia William4; Abeer Abdel Rahman51Medical Parasitology, Faculty of Medicine, Ain Shams university2Parasitology Dept., Faculty of Medicine, Ain Shams Univ.3Department of Medical Parasitology, Faculty of Medicine,Ain Shams University.4parasitology department,TBRI, Cairo, Egypt.5Parasitology department,Faculty of Medicine, Ain shams university, cairo, EgyptAbstractSearching for a new immunomodulating supportive aid for the sole drug of choice to schistosomiasis mansoni, praziquantel, is crucial.The aim of the present work is to assess the immunomodulatory effect of Kalobin on schistosomiasis mansoni in vivo.
The study included 80 mice, 20 in the control groups and 60 in the experimental groups. Mice were divided into two major categories immunocompetent and immunosuppressed. The study started by a preliminary trial including 5 IC mice given a combination of 100 mg/kg of each of the 2 drugs PZQ and Kalobin. According to the results obtained, settled groups for IC mice included: 5 mice given 200 mg/kg PZQ, 6 mice given 50 mg/kg PZQ, 5 mice given 200 mg/kg Kalobin, 5 mice given a 50 mg/kg combination of each drug. The settled subgroups for IS drugs included: 5 mice given 200 mg/kg PZQ, 6 mice given 50 mg/kg PZQ, 6 mice given 200 mg/kg Kalobin, and 5 mice given a 50 mg/kg combination of each drug. All mice were sacrificed nine wpi. Assessment included parasitological parameters, measurement of hepatic granuloma number and size. Immunohistochemical procedure to detect the expression of VEGF.Therapy with Kalobin combined to PZQ proved to be superior to the sole PZQ, as shown by its effect on TWB and oogram pattern and greater reduction in intestinal and hepatic egg counts in IS groups. The combination (50 mg/kg each) approached the individual higher PZQ dose (200 mg/kg) in reducing the granuloma number and was even better in reducing VEGF expression in IC groups with a better impact in IS groups. Individual Kalobin had an unexpected moderate potential effect.Keywords Anti-schistosomal; Kalobin; praziquantel; S. mansoni; experimental study; immunomodulation; VEGF Main Subjects Helminthology

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