ABDELTAWAB, M., ABDEL-SHAFI, I., ABOULHODA, B., WANAS, H., SAAD EL-DIN, S., AMER, S., HAMED, A. (2022). Investigating the effect of the nitric oxide donor L-arginine on albendazole efficacy in Trichinella spiralis-induced myositis and myocarditis in mice. Parasitologists United Journal, (), -. doi: 10.21608/puj.2022.119638.1153

MAGDA ABDELTAWAB; IMAN ABDEL-SHAFI; BASMA ABOULHODA; HANAA WANAS; SHIMAA SAAD EL-DIN; SAMAR I.I AMER; ALSHAIMAA M.R HAMED. "Investigating the effect of the nitric oxide donor L-arginine on albendazole efficacy in Trichinella spiralis-induced myositis and myocarditis in mice". Parasitologists United Journal, , , 2022, -. doi: 10.21608/puj.2022.119638.1153

ABDELTAWAB, M., ABDEL-SHAFI, I., ABOULHODA, B., WANAS, H., SAAD EL-DIN, S., AMER, S., HAMED, A. (2022). 'Investigating the effect of the nitric oxide donor L-arginine on albendazole efficacy in Trichinella spiralis-induced myositis and myocarditis in mice', Parasitologists United Journal, (), pp. -. doi: 10.21608/puj.2022.119638.1153

ABDELTAWAB, M., ABDEL-SHAFI, I., ABOULHODA, B., WANAS, H., SAAD EL-DIN, S., AMER, S., HAMED, A. Investigating the effect of the nitric oxide donor L-arginine on albendazole efficacy in Trichinella spiralis-induced myositis and myocarditis in mice. Parasitologists United Journal, 2022; (): -. doi: 10.21608/puj.2022.119638.1153

Articles in Press, Accepted Manuscript, Available Online from 28 March 2022   Document Type: Original Article DOI: 10.21608/puj.2022.119638.1153Authors MAGDA ABDELTAWAB1; IMAN ABDEL-SHAFI2; BASMA ABOULHODA3; HANAA WANAS4; SHIMAA SAAD EL-DIN5; SAMAR I.I AMER6; ALSHAIMAA M.R HAMED 71medical parasitology, faculty of medicine, Cairo university, Cairo, Egypt.2Medical Parasitology, Kasr Al Ainy Faculty of Medicine, Cairo University3Anatomy & Embryology Department, Faculty of Medicine Cairo University4Pharmacology Department, Faculty of Medicine Cairo University5Biochemistry Department, Faculty of Medicine Cairo University6Pathology Department, Faculty of Medicine Cairo University.7Department of Medical Parasitology, Faculty of Medicine, Cairo UniversityAbstractBackground:The immune response against Trichinella spiralis results in a mixed cytokine state and nitric oxide (NO) production. Albendazole (ALB) influences the oxidative status and cytokine profile of the host, in addition to its direct action on the parasite beta-tubulin
Objective: The current study was designed to assess the effect of the NO donor, L-arginine, on the muscular phase of experimental trichinosis in mice with and without ALB administration.
Material and Methods: The study included five groups (G); negative non-infected control group (G1); positive infected non-treated control group (G2); ALB-treated group (G3); L-arginine-treated group (G4); and combined ALB and L-arginine regimen group (G5). Study parameters included larval count, histopathological changes, and NO synthase (iNOS) immunohistochemical expression in skeletal muscle and heart during the muscular phase. Gene expression levels of TNF and IFN, and NO production were also evaluated by quantitative RT-qPCR.
Results: L-arginine resulted in a significant increase in tissue iNOS expression and serum NO levels, in addition to the increase in TNF and INF gene expression. Albendazole, on the other hand, was found to decrease local tissue expression of iNOS and serum NO in infected mice, whereas gene expression levels of TNF and IFN were elevated.
Conclusion: Nitric oxide homeostasis is vital to the infection outcome and regeneration during muscular trichinellosis. Albendazole exerts immunomodulatory effects in addition to its direct anti-parasitic action on T. spiralis.Keywords Key Words: Albendazole; immunohistochemistry; inflammation; L-arginine; muscular phase; nitric oxide; RT-qPCR; Trichinella spiralis Main Subjects Helminthology

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