Effects of oryzalin on cortical microtubules in cesa3 mutants

To determine whether mutations in CESA3 subunit affect cortical microtubule stability, microtubule depolymerization by oryzalin was assessed. In this study, A. thaliana root apex was classified into four distinct zones, the meristematic, transition (also referred as distal elongation zone), fast elongation (also known as elongation zone) and growth terminating zone, according to Verbelen et al. [39]. Epidermal cells of the transition and fast elongation zone were studied for cortical microtubule stability, since cell divisions in them are scarce and microtubules are uniformly distributed. Besides, as one of the first effects of oryzalin is the disruption of the cell division-specific microtubule arrays [40,41,42,43], the meristematic zone was also monitored to verify whether seedlings were affected by the drug. The features revealing cortical microtubule stability or susceptibility in the transition and fast elongation zone were their integrity, as exhibited by the degree of fragmentation, and their density in each cell, as revealed by the relevant fluorescence intensity measurement. The orientation and density of cortical microtubules in wild-type seedlings, as well as in the heterozygous than and homozygous, rsw1 and prc1-1 mutants, have been shown in Panteris et al. [28, 29].

Firstly, we investigated cortical microtubule stability in heterozygous than (cesa3; referred to as than/+) seedlings. Cortical microtubules of than/+ roots, under any procedure of treatment (see Methods), appeared more stable than those of the wild-type (Figs. 1 and 2). In the meristematic zone of both wild-type and than/+, abnormal cell divisions, disoriented phragmoplasts and incomplete cell walls could be observed (Figs. 1a and c and 2a, b). In transition and fast elongation zone cells of than/+ roots, cortical microtubules were more integral and densely arranged, compared to those of the wild-type (Figs. 1b, d and 2c, d). The integrity of cortical microtubules was further verified by the fluorescence intensity measurements (Fig. 3). Taken together, cortical microtubules appear more stable in than/+ root cells than in those of the wild-type. This indicates that there might be a correlation between cortical microtubule stability and CESA function or cellulose content, as than/+ is characterized by reduced cellulose content and whole plant dwarfism [14].

Fig. 1

Comparison of microtubule integrity between the wild-type and than/+. Effects of oryzalin on 4-5-d-old wild-type (a, b) and than/+ (c, d) roots. Seedlings were treated for 4 h with 400 nM oryzalin. In the meristematic zone, cell divisions are affected (a, c arrows). Cortical microtubules in transition and fast elongation zone cells of than/+ appear more integral than those of the wild-type (d; cf. b). In all the Figures the insets show immunostained microtubules at higher magnification. Scale bars: 20 μm

Fig. 2

Comparison of microtubule integrity between the wild-type and than/+. Effects of oryzalin on wild-type (a, c) and than/+ (b, d) roots. Seedlings were transplanted from control medium to substrate with oryzalin 200 nM for 6 h. Cell divisions in the meristematic zone of both wild-type and than/+ are affected, as incomplete cell walls and abnormal phragmoplasts can be observed (a, b arrows). Cortical microtubules are longer and more integral in transition and fast elongation zone cells of than/+ compared to wild-type roots (d; cf. c). Scale bars: 20 μm

Fig. 3

Fluorescence intensity measurements of cortical microtubules in the transition and fast elongation zone of wild-type and than/+ roots. Maximum intensity projections of serial CLSM sections of transition zone and fast elongation zone were used. Increased fluorescence intensity in than/+ was observed. (a) Effect of 4 h treatment with 400 nM oryzalin. (b) Effect of oryzalin on roots transplanted from control medium to substrate with oryzalin 200 nM for 6 h. Error bars indicate standard error. The intensity difference was statistically significant compared to control group (oryzalin-treated Col) (t-test, p < 0.001, n = 10). In all the figures the n corresponds to the number of cells measured in each root tip. At least three root tips were used for fluorescence intensity measurements

Subsequently, we investigated whether the increased resistance to oryzalin is restricted to the than/+ mutant only, or it is a general feature of cesa3 mutants. Therefore, the mre1 as well as pcesa3, a transgenic plant expressing the than allele [14], were treated with 400 nM oryzalin for 4 h. Both mutants exhibited cortical microtubules more persistent against oryzalin than the wild-type. Specifically, in transition and fast elongation zone cells of the mutants, cortical microtubules appeared longer and more integral (Fig. 4b, c) than those of wild-type roots (Fig. 4a). In addition, higher fluorescence intensity, as a result of more tubulin polymers, was observed in the mutants, compared to the wild-type (Additional file 1: Fig. S1), which confirms the presence of more and longer microtubules. These observations indicate that cortical microtubule stability is a common feature among cesa3 mutants.

Fig. 4

Comparison of microtubule integrity between the wild-type and mre1 and pcesa3. Effects of 4 h treatment with 400 nM oryzalin on 4-5-d-old wild-type (a), mre1 (b) and pcesa3 (c) roots. In both mutants (b, c) cortical microtubules of transition and fast elongation zone cells appear longer and more integral, compared to those of wild-type roots (a). Scale bars: 20 μm

The effect of oryzalin on cortical microtubules in cesa1 and cesa6 mutants

In order to examine whether cortical microtubule stability is limited to cesa3 mutants or it might be a general feature of primary cell wall-related cesa mutants, rsw1 and any1 (cesa1 mutants), as well as prc1-1 (cesa6 mutant) seedlings were treated with 200 nM oryzalin for 6 h. In the transition and fast elongation zones of any1 and prc1-1, more integral microtubules could be observed (Fig. 5b, c), compared to those of wild-type roots. At 30 °C, root cells of rsw1 showed reoriented but integral cortical microtubules, after treatment with oryzalin, while wild-type roots exhibited fewer and shorter microtubules under the same treatment (Fig. 5e; cf. 5d). The increased persistence of cortical microtubules against oryzalin in the mutants was also confirmed by fluorescence intensity quantification (Additional file 2: Fig. S2). It can, therefore, be concluded that increased cortical microtubule persistence against oryzalin is a common feature of primary cell wall-related cesa mutants.

Fig. 5

Comparison of microtubule integrity between the wild-type and cesa1 and cesa6 mutants. Effects of 6 h 200 nM oryzalin treatment after transplantation of 5-7-d-old wild-type, any1, prc1-1 and rsw1 seedlings to medium containing the drug. Seedlings in (d) and (e) were treated at 30 oC, while the others at room temperature. Cortical microtubules in any1 and prc1-1 appear less affected, compared to the wild-type (b and c, compare to a). In any1 and prc1-1 cortical microtubules are longer and more densely arranged, compared to the wild-type. In rsw1 cortical microtubules appear more integral than in the wild-type at 30 oC. Scale bars: 20 μm

Our results are different from those of Paredez et al., who described the prc1-20 (cesa6 mutant) as hypersensitive to oryzalin [25]. This statement was supported by reorientation of cortical microtubules in root cells, as well as by root swelling when exposed to oryzalin. The differences between our findings and the above may be due to different observation approaches and/or the specific criteria for assessing stability. In the above work [25], a GFP-MAP4 marker was used to visualize microtubules, while here α-tubulin immunostaining was performed. Microtubule-Associated Protein 4 (MAP4) decorates microtubules and its fusion to GFP allows microtubule visualization [24]. It has been considered that overexpression of GFP-MAP4 fusion proteins may produce an altered developmental phenotype of microtubule dynamics and orientation [24, 44]. In contrast to GFP markers, immunostaining provides more precise and exact microtubule phenotype as it does not interfere with expression and/or function.

In addition, Paredez et al. did not observe any microtubule disassembly but reorientation [25]. In our study, however, cortical microtubules underwent depolymerization without reorientation. In terms of the properties of anti-microtubule drugs, this behavior appears more expected [40] than microtubule reorientation.

The effect of colchicine on cortical microtubules of cesa1, cesa3 and cesa6

In order to further investigate whether the increased cortical microtubule stability of the above cesa mutants is limited to oryzalin or it is generally manifested against anti-microtubule drugs, treatments with colchicine, another microtubule-depolymerizing drug were administered. Colchicine is the most “classic” and “notorious” drug that disrupts microtubules (among others [45, 46]). Wild-type, any1, than/+ and prc1-1 seedlings were treated with 2 mM colchicine for 1.5 h. The concentration of the drug applied was much higher than that of oryzalin, since colchicine was reported to be effective on plants at higher concentrations (among others [45, 47]). All the seedling roots affected by colchicine showed extensive microtubule depolymerization (Fig. 6). The microtubule remnants were scarce and short. However, the fragments of microtubules appeared longer in the mutants than in the wild-type (Fig. 6b–d, cf. 6a). These results were further verified by the fluorescence intensity measurements (Additional file 3: Fig. S3). Increased cortical microtubule stability may thus be considered as a general feature among cesa mutants, irrespective of the drug or the CESA subunit mutation.

Fig. 6

Effects of colchicine on 7-d-old wild-type (a), any1 (b), than/+ (c) and prc1-1 (d). Transition and fast elongation zone cells were examined, after transplantation to medium containing 2 mM colchicine for 1.5 h. Cortical microtubules are extensively disrupted and only remnants can be observed in some cells (arrows). These microtubule remnants are longer and exhibit higher density in any1, than/+ and prc1-1 cells, compared to those of wild-type roots (b–d, compare to a). Scale bars: 20 μm

Cortical microtubule stability in the pom2-4 mutant

So far, mutants with deficient CESA subunits and decreased cellulose synthesis, except any1, which shows reduced cell wall crystallinity and CESA velocity but physiological cellulose content [15], have been studied. In order to discriminate if the increased microtubule stability resulted from malfunction of the CESAs per se or from defective cellulose synthesis, the csi1 mutant pom2-4 was treated with oryzalin. In this csi1 mutant, defects in growth and cell expansion occur due to decreased cellulose content, although the CESA subunits are normal [19, 20, 48]. After treatment with 200 nM oryzalin for 6 h, cell divisions appeared affected in the meristematic zone of both wild-type and pom2-4 roots (Fig. 7a, c). In transition and fast elongation zone of roots treated as above, cortical microtubules of pom2-4 appeared longer and more integral, in comparison with those of the wild-type (Fig. 7d, cf. 7b). Increased fluorescence intensity was observed in pom2-4, as well (Additional file 2: Fig. S2). Contrarily to our results, Mei et al. reported that cortical microtubules in hypocotyl cells of csi1-2 were more sensitive to oryzalin than those of the wild-type [30]. However, their observations were made by GFP-MAP4 imaging, which may affect microtubule properties and behavior (see above). In addition, root cells may react differently to oryzalin than hypocotyl cells. Taken together, our observations support that increased cortical microtubule stability, which is common in cesa and csi1 mutants, should rather be attributed not to CESA malfunction per se, but to decreased cellulose content in the cell wall.

Fig. 7

Comparison of microtubule integrity between the wild-type and pom2-4. Microtubule integrity in 5-d-old wild-type (a, b) and pom2-4 (c, d) roots, after transplantation to medium containing 200 nM oryzalin for 6 h. Cell divisions in the meristematic root zone are affected by the drug (arrows in a and c). In transition and fast elongation zone cells, pom2-4 exhibits longer and more integral cortical microtubules (d), in comparison to the wild-type, the microtubules of which appear depolymerized and display intense background fluorescence (b). Scale bars: 20 μm

Inhibition of cellulose synthesis increases cortical microtubule stability in wild-type seedlings

To address the question whether defective cellulose biosynthesis may improve cortical microtubule stability, wild-type roots were examined after combined treatment with DCB and oryzalin. DCB is a synthetic herbicide causing cessation of CESA complex movement, resulting in inhibition of cellulose synthesis and deposition (among others [49, 50]). For this experiment, 4-d-old wild-type seedlings were transferred to a medium containing 400 nM DCB for 22 h and then transferred to medium with 400 nM DCB and 200 nM oryzalin for 6 h. Seedlings treated for 6 h with 200 nM oryzalin alone were used as control (Fig. 8). Root tip bulging and inhibition of seedling growth was observed in DCB and combined DCB and oryzalin treatment (Additional file 4: Fig. S4). Abnormal cell divisions and incomplete cell walls in oryzalin and in the combined treatment prove the effect of oryzalin in the roots (Fig. 8a, b). The length of all root cell types appeared affected after treatment with DCB, and the cells appeared shorter and flattened, compared to untreated root cells (Fig. 8b; cf. 8a, 8d, 8e; cf. 8c). As shown in Fig. 8d, DCB did not affect cortical microtubules. Wild-type roots subjected to combined treatment exhibited cortical microtubules as integral as roots treated with DCB only in the transition and fast elongation zones (Fig. 8e; cf. 8d), not depolymerized as in oryzalin-treated roots (Fig. 8c). Increased fluorescence intensity was observed in DCB and combined DCB and oryzalin treatments compared to the control (Fig. 9).

Fig. 8

DCB treatment increases microtubule resistance to oryzalin. Effects of combined treatment with DCB and oryzalin on wild-type roots. a, b Effect of 6 h treatment with 200 nM oryzalin on meristematic zone cells without a or after b 400 nM DCB treatment. Disordered phragmoplasts and incomplete cell walls can be observed in both cases (a, b). c Oryzalin-affected transition/fast elongation zone cells exhibit disrupted and depolymerized cortical microtubules. d Roots of seedlings transplanted for 28 h in medium with 400 nM DCB exhibit short cells with unaffected cortical microtubules. e In roots treated for 6 h with 400 nM DCB and 200 nM oryzalin, after been transplanted for 22 h in medium containing 400 nM DCB, cortical microtubules appear unaffected by oryzalin in the transition and fast elongation zone (e compare to d). Scale bars: 20 μm

Fig. 9

Fluorescence intensity measurements of cortical microtubules of wild-type roots. Prior to 200 nM oryzalin treatment for 6 h, seedlings were transplanted to media containing either 400 nM DCB for 28 h or 10 mg L− 1 Congo red for 22 h. Oryzalin-treated seedlings showed the lowest fluorescence intensity. The fluorescence intensity difference was statistically significant for DCB, Congo red and combined treatment of Congo red and oryzalin, compared to the control (p < 0.0001, n = 10). No statistical significance was observed for intensity fluorescence when oryzalin-treated and combined oryzalin- and DCB-treated roots were compared (p = 0.6835, n = 10). Error bars indicate standard error

Inhibition of cell expansion results in more stable cortical microtubules in the transition and fast elongation zones

A common feature of cellulose-deficient mutants, as well as of DCB-treated wild-type seedlings, is the inhibition of cell elongation [9, 10, 14, 34, 49]. In addition, although the any1 mutant of cesa1 is characterized by normal cellulose content, it exhibits decreased cell elongation due to defective cellulose crystallinity [15]. In order to clarify whether inhibition of cell elongation might be the main cause for increased microtubule stability, the effect of Congo red, a dye that inhibits cell expansion, was investigated (Fig. 10). Congo red, binds to cellulose without affecting its biosynthesis but preventing glucan chain crystallization and, consequently, typical microfibril formation [32, 33, 51]. Wild-type seedlings were treated either with 200 nM oryzalin for 6 h, or they were first transplanted for 22 h to medium supplemented with 10 mg L− 1 Congo red and then transplanted to medium containing 10 mg L− 1 Congo red and 200 nM oryzalin for 6 h. Root tips of seedlings treated with Congo red or Congo red and oryzalin exhibited intense curling (Additional file 4: Fig. S4). Abnormal cell divisions observed in both oryzalin and combined treatments revealed the depolymerizing effect of oryzalin (Fig. 10a, b). Congo red application resulted in decreased cell elongation in the root apex developmental zones without affecting cortical microtubules (Fig. 10d). After the combined Congo red and oryzalin treatment, cortical microtubules in transition and fast elongation zone cells appeared integral and almost unaffected by oryzalin (Fig. 10e; cf. 10d), in contrast to those of roots treated with oryzalin alone, in which cortical microtubules appeared fragmented and partially depolymerized (Fig. 10c). The increased integrity in Congo red and combined Congo red and oryzalin treatments, compared to the control, was verified by fluorescence intensity measurements (Fig. 9) and was found statistically significant (p < 0.0001). According to these findings, it may be concluded that inhibition of cell expansion, irrespective of the cause, results in increased microtubule stability.

Fig. 10

Congo red treatment increases microtubule resistance to oryzalin. Wild-type seedlings treated with Congo red and oryzalin. a, b Cell divisions (arrows) appear affected in the meristematic zone of roots treated for 6 h with 200 nM oryzalin (a) or with 10 mg L− 1 Congo red and 200 nM oryzalin for 6 h (b). c Transition/fast elongation zone cells after 6 h exposure to 200 nM oryzalin exhibit fragmented and partially depolymerized cortical microtubules. d Congo red treatment (10 mg L− 1 for 22 h) does not affect cortical microtubules. e Combined treatment as referred in b. Cortical microtubules appear integral, not disrupted as in roots treated with oryzalin alone (e compare to c). Scale bars: 20 μm

Fisher and Cyr were the first to describe a bidirectional relationship between cortical microtubules and the cell wall [23]. According to them, the mechanical properties of cell wall, rather than cellulose biosynthesis, affect cortical microtubule stability and organization. When Nicotiana tabacum BY-2 protoplasts were treated with isoxaben (a cellulose biosynthesis inhibitor), microtubules were randomly organized. Cultivation in the absence of isoxaben resulted in highly organized cortical microtubules [23]. Since then, it was supported by several authors that cellulose biosynthesis provides spatial cues for cortical microtubule organization. Chu et al. and Paredez et al. reported alterations in cortical microtubule organization and stability in cesa mutants, concluding that cellulose synthesis may play a regulatory role in microtubule organization [24, 25]. On the other hand, Le et al. supported that inhibition of cell elongation in ethylene-treated roots results in microtubule reorientation [27]. In agreement with the latter work, Panteris et al., have demonstrated that chemical, mechanical or genetic inhibition of cell expansion affects cortical microtubule orientation in A. thaliana root apex, supporting the bidirectional relationship of cell wall and cortical microtubules [28, 29].

The results of this study support that cell expansion is associated not only with cortical microtubule orientation [28, 29] but also with microtubule stability. The common feature among cesa mutants (any1, rsw1, than/+, mre1, prc1-1), csi1 mutant (pom2-4), DCB and Congo red treatments is that, in all cases, cell expansion is negatively affected. More specifically, decreased cellulose biosynthesis, either caused by CESA malfunction (any1, rsw1, than/+, mre1, prc1-1 and DCB), or by CSI1 (pom2-4) mutation, results in inhibition of cell elongation [9, 10, 14, 34, 48,49,50]. On the other hand, Congo red prevents cellulose crystallization [32, 33], while any1 shows altered cell wall crystallinity [