B Alhatlani and W Aljabr designed the study, analyzed and interpreted the results, and drafted the manuscript. M Almarzouqi, S Alhatlani, R Alzunaydi, A Alsaykhan, A Almusallam, and S Almaiman collected the samples and performed experiments. AH Aleid, A Alsughayir and Y Bishawri conducted the statistical analysis. All authors read and approved the final manuscript.