Preeclampsia is a "placenta-derived disorder" characterized disease, which remains a major unaddressed public health problem. The differentiation and development of cytotrophoblasts and extravillous trophoblasts are two crucial processes, which are tightly regulated. And any abnormal regulation can lead to the occurrence of pregnancy-related diseases such as preeclampsia. In this article, we performed Spatial transcriptomics on tissues of PE and Normal, and found a large amount of extravillous trophoblasts (EVTs) were accumulated in the cluster 8 (cytotrophoblast shell) of PE_decidua, and Trajectory analysis revealed KRT8 was over-expressed in the cytotrophoblast shell of PE_decidua. The accumulation of EVTs caused by the increase of KRT8 promotes the development of preeclampsia.

The authors have declared no competing interest.

not applicable

Fund of Hubei Province Chutian Talent Program; Major science and technology projects in Hubei Province?No.2022AC005?; Hubei Provincial Department of Science and Technology (No. 2022CFB561)

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The details of the IRB/oversight body that provided approval or exemption for the research described are given below:

The study was approved by the Ethics committee of Maternal and Child Health Hospital of Hubei Province (2021XM049).

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The datasets used and analyzed during the current study are available from the corresponding author on reasonable request