The CYP2C19 gene single nucleotide polymorphisms (SNPs) should be considered in the clinical use of clopidogrel, which has important guiding value for predicting the risk of bleeding and thrombosis after clopidogrel treatment. The CRISPR/Cas system is increasingly used for SNP detection due to its single nucleotide mismatch specificity. Simultaneous detection of multiple SNPS for rapid identification of the CYP2C19 genotype is important, but there is no method to detect a wide variety of CYP2C19 genes at the same time. This paper proposed a new integrated system, by integrating PCR reaction and CRISPR/Cas detection of three CYP2C19 genes on a device, achieving rapid, sensitive, and specific detection. In our design, magnetic beads with three different sizes capture target nucleic acid from the sample, dragging through the different areas by the magnetic force, for CYP2C19*2, CYP2C19* 3 and CYP2C19 * 17 genes of PCR amplification reaction and CRISPR/Cas 13 a detection respectively. Magnetic beads were sorted by microporous PC membranes of different apertures. The study has a broad clinical application prospect, providing a favorable tool for clinical clopidogrel administration.

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