The advent of single cell DNA sequencing revealed astonishing dynamics of genomic variability, but failed at characterizing smaller to mid size variants that on the germline level have a profound impact. In this work we discover novel dynamics in three brains utilizing single cell long-read sequencing. This provides key insights into the dynamic of the genomes of individual cells and further highlights brain specific activity of transposable elements.

FJS receives research support from PacBio, Illumina and Oxford Nanopore.

The study is funded in part by the joint efforts of The Michael J. Fox Foundation for Parkinsons Research (MJFF) and the Aligning Science Across Parkinsons (ASAP) initiative. MJFF administers the grant [Grant ID 000430] on behalf of ASAP and itself. This study is in part supported by NIH (UG3NS132105).

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Yes

The details of the IRB/oversight body that provided approval or exemption for the research described are given below:

All donors had given informed consent for the use of their brain in research and ethics approval was provided tissue bank by the UK National Research Ethics Service (07/MRE09/72). All ethical regulations relevant to human research participants were followed.

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