World malaria report 2023. World Health Organization. 2023. https://www.who.int/teams/global-malaria-programme/reports.

Taylor TE, Fu WJ, Carr RA, Whitten RO, Mueller JS, Fosiko NG, et al. Differentiating the pathologies of cerebral malaria by postmortem parasite counts. Nat Med. 2004;10:143–5.

Article  CAS  PubMed  Google Scholar 

Rask TS, Hansen DA, Theander TG, Pedersen AG, Lavstsen T. Plasmodium falciparum erythrocyte membrane protein 1 diversity in seven genomes - divide and conquer. PLoS Comput Biol. 2010;6: e1000933.

Article  PubMed  PubMed Central  Google Scholar 

Lavstsen T, Gilbert MTP, Willerslev E, Baraka V, Theander TG, Marquard AM, et al. Plasmodium falciparum erythrocyte membrane protein 1 domain cassettes 8 and 13 are associated with severe malaria in children. Proc Natl Acad Sci. 2012;109:e1791–800.

Article  CAS  PubMed  PubMed Central  Google Scholar 

Kessler A, Dankwa S, Bernabeu M, Harawa V, Danziger SA, Duffy F, et al. Linking EPCR-binding PfEMP1 to brain swelling in pediatric cerebral malaria. Cell Host Microbe. 2017;22:601-614.e5.

Article  CAS  PubMed  PubMed Central  Google Scholar 

Lennartz F, Adams Y, Bengtsson A, Olsen RW, Turner L, Ndam NT, et al. Structure-guided identification of a family of dual receptor-binding PfEMP1 that is associated with cerebral malaria. Cell Host Microbe. 2017;21:403–14.

Article  CAS  PubMed  PubMed Central  Google Scholar 

Tuikue Ndam N, Moussiliou A, Lavstsen T, Kamaliddin C, Jensen ATR, Mama A, et al. Parasites causing cerebral falciparum malaria bind multiple endothelial receptors and express EPCR and ICAM-1-binding PfEMP1. J Infect Dis. 2017;215:1918–25.

Article  PubMed  Google Scholar 

Adams Y, Olsen RW, Bengtsson A, Dalgaard N, Zdioruk M, Satpathi S, et al. Plasmodium falciparum erythrocyte membrane protein 1 variants induce cell swelling and disrupt the blood–brain barrier in cerebral malaria. J Exp Med. 2021;218: e20201266.

Article  CAS  PubMed  PubMed Central  Google Scholar 

Kanoi BN, Nagaoka H, Morita M, White MT, Palacpac NMQ, Ntege EH, et al. Comprehensive analysis of antibody responses to Plasmodium falciparum erythrocyte membrane protein 1 domains. Vaccine. 2018;36:6826–33.

Article  CAS  PubMed  Google Scholar 

Rambhatla JS, Tonkin-hill GQ, Takashima E, Tsuboi T, Noviyanti R, Trianty L, et al. Identifying targets of protective antibodies against severe malaria in Papua, Indonesia, using locally expressed domains. Infect Immun. 2022;90: e0043521.

Article  PubMed  Google Scholar 

Travassos MA, Niangaly A, Bailey JA, Ouattara A, Coulibaly D, Lyke KE, et al. Children with cerebral malaria or severe malarial anaemia lack immunity to distinct variant surface antigen subsets. Sci Rep. 2018;8:6281.

Article  PubMed  PubMed Central  Google Scholar 

Obeng-Adjei N, Larremore DB, Turner L, Ongoiba A, Li S, Doumbo S, et al. Longitudinal analysis of naturally acquired PfEMP1 CIDR domain variant antibodies identifies associations with malaria protection. JCI Insight. 2020;5: e137262.

Article  PubMed  PubMed Central  Google Scholar 

Tessema SK, Utama D, Chesnokov O, Hodder AN, Lin CS, Harrison GLA, et al. Antibodies to intercellular adhesion molecule 1-binding Plasmodium falciparum erythrocyte membrane protein 1-DBLβ are biomarkers of protective immunity to malaria in a cohort of young children from Papua New Guinea. Infect Immun. 2018;86:1–14.

Article  Google Scholar 

Tessema SK, Nakajima R, Jasinskas A, Monk SL, Lekieffre L, Lin E, et al. Protective immunity against severe malaria in children is associated with a limited repertoire of antibodies to conserved PfEMP1 variants. Cell Host Microbe. 2019;26:579-590.e5.

Article  CAS  PubMed  Google Scholar 

Chan JA, Boyle MJ, Moore KA, Reiling L, Lin Z, Hasang W, et al. Antibody targets on the surface of Plasmodium falciparum-infected erythrocytes that are associated with immunity to severe malaria in young children. J Infect Dis. 2019;219:819–28.

Article  CAS  PubMed  Google Scholar 

Zelter T, Strahilevitz J, Simantov K, Yajuk O, Adams Y, Jensen AR, et al. Neutrophils impose strong immune pressure against PfEMP1 variants implicated in cerebral malaria. EMBO Rep. 2022;23: e53641.

Article  CAS  PubMed  PubMed Central  Google Scholar 

Arora G, Hart GT, Manzella-Lapeira J, Doritchamou JY, Narum DL, Thomas LM, et al. NK cells inhibit Plasmodium falciparum growth in red blood cells via antibody-dependent cellular cytotoxicity. Elife. 2018;7:1–20.

Article  Google Scholar 

Arnold KB, Chung AW. Prospects from systems serology research Immunology. 2018;153:279–89.

CAS  PubMed  Google Scholar 

Aitken EH, Damelang T, Ortega-Pajares A, Alemu A, Hasang W, Dini S, et al. Developing a multivariate prediction model of antibody features associated with protection of malaria-infected pregnant women from placental malaria. Elife. 2021;10:1–30.

Article  Google Scholar 

Tonkin-Hill GQ, Trianty L, Noviyanti R, Nguyen HHT, Sebayang BF, Lampah DA, et al. The Plasmodium falciparum transcriptome in severe malaria reveals altered expression of genes involved in important processes including surface antigen–encoding var genes. PLoS Biol. 2018;16: e2004328.

Article  PubMed  PubMed Central  Google Scholar 

Olsen RW, Ecklu-Mensah G, Bengtsson A, Ofori MF, Lusingu JP, Castberg FC, et al. Natural and vaccine-induced acquisition of cross-reactive IgG-inhibiting ICAM-1-specific binding of a Plasmodium falciparum PfEMP1 subtype associated specifically with cerebral malaria. Infect Immun. 2018;86:1–17.

Article  CAS  Google Scholar 

Harawa V, Njie M, Kessler A, Choko A, Kumwenda B, Kampondeni S, et al. Brain swelling is independent of peripheral plasma cytokine levels in Malawian children with cerebral malaria. Malar J. 2018;17:435.

Article  CAS  PubMed  PubMed Central  Google Scholar 

Molyneux ME, Taylor TE, Wirimaf JJ, Borgsteinj A. Clinical features and prognostic indicators in paediatric cerebral malaria a study of 131 comatose Malawian children. Q J Med. 1989;71:441–59.

CAS  PubMed  Google Scholar 

Olsen RW, Ecklu-Mensah G, Bengtsson A, Ofori MF, Kusi KA, Koram KA, et al. Acquisition of IGG to ICAM-1-binding DBLΒ domains in the Plasmodium falciparum erythrocyte membrane protein 1 antigen family varies between groups A, B, and C. Infect Immun. 2019;87:e00224-e319.

Article  CAS  PubMed  PubMed Central  Google Scholar 

Bengtsson A, Joergensen L, Rask TS, Olsen RW, Andersen MA, Turner L, et al. A novel domain cassette identifies Plasmodium falciparum PfEMP1 proteins binding ICAM-1 and is a target of cross-reactive, adhesion-inhibitory antibodies. J Immunol. 2013;190:240–9.

Article  CAS  PubMed  Google Scholar 

Walker IS, Chung AW, Damelang T, Rogerson SJ. Analysis of antibody reactivity to malaria antigens by microsphere-based multiplex immunoassay. In: Hviid L, editor. Jensen ATR. Malaria immunology. Methods in molecular biology. New York: Humana; 2022. p. 309–25.

Google Scholar 

Wines BD, Vanderven HA, Esparon SE, Kristensen AB, Kent SJ, Hogarth PM. Dimeric FcγR ectodomains as probes of the Fc receptor function of anti-influenza virus IgG. J Immunol. 2016;197:1507–16.

Article  CAS  PubMed  Google Scholar 

Jensen JB. In vitro culture of Plasmodium parasites. In: Doolan DL, editor. Malaria methods and protocols. Totowa: Humana; 2002. p. 477–88.

Chapter  Google Scholar 

Joergensen L, Bengtsson DC, Bengtsson A, Ronander E, Berger SS, Turner L, et al. Surface co-expression of two different PfEMP1 antigens on single plasmodium falciparum-infected erythrocytes facilitates binding to ICAM1 and PECAM1. PLoS Pathog. 2010;6: e1001083.

Article  PubMed  PubMed Central  Google Scholar 

Lennartz F, Bengtsson A, Olsen RW, Joergensen L, Brown A, Remy L, et al. Mapping the binding site of a cross-reactive Plasmodium falciparum PfEMP1 monoclonal antibody inhibitory of ICAM-1 binding. J Immunol. 2015;195:3273–83.

Article  CAS  PubMed  PubMed Central  Google Scholar 

Ataíde R, Hasang W, Wilson DW, Beeson JG, Mwapasa V, Molyneux ME, et al. Using an improved phagocytosis assay to evaluate the effect of HIV on specific antibodies to pregnancy-associated malaria. PLoS ONE. 2010;5: e10807.

Article  PubMed  PubMed Central