Evaluation of anticancer activity of novel platinum(II) bis(thiosemicarbazone) complex against breast cancer

ElsevierVolume 148, July 2024, 107486Bioorganic ChemistryAuthor links open overlay panel, , , , , , , , , Highlights•

Bis(thiosemicarbazone) derivatives with/without platinum were synthesized.

TioPt exhibited the most potent anticancer properties against breast cancer cells.

TioPt had a sparing effect against MCF-10A normal breast epithelial cells.

Pt(II) complex induced apoptosis (↑ caspase 8 and 3/7 activity, ↓ ΔΨm) in cells.

The tested derivatives were not activators of autophagy in breast cancer cells.

Abstract

The study aims to synthesize a novel bis(thiosemicarbazone) derivative based on platinum (thioPt) and evaluate its anticancer properties against MFC-7 and MDA-MB-231 breast cancer cells. A new platinum complex was synthesised by reacting K2PtCl4 with 2,2′-(1,2-diphenylethane-1,2-diylidene)bis(hydrazine-1-carbothioamide) in ethanol in the presence of K2CO3. In the obtained complex, the platinum atom is coordinated by a conjugated system = N-Ndouble bondC-S-The structures of the new compound were characterised using NMR spectroscopy, HR MS, IR, and X-ray structural analysis. The obtained results of the cytotoxicity assay indicate that compound thioPt had potent anticancer activity (MCF-7: 61.03 ± 3.57 µM, MDA-MB-231: 60.05 ± 5.40 µM) with less toxicity against normal MCF-10A breast epithelial cells, even compared to the reference compound (cisplatin). In addition, subsequent experiments found that thioPt induces apoptosis through both an extrinsic (↑caspase 8 activity) and intrinsic (↓ΔΨm) pathway, which ultimately leads to an increase in active caspase 3/7 levels. The induction of autophagy and levels of proteins involved in this process (LC3A/B and Beclin-1) were examined in MCF-7 and MDA-MB-231 breast cancer cells exposed to tested compounds (thio, thioPt, cisPt) at a concentration of 50 µM for 24 h. Based on these results, it can be concluded that thio and thioPt do not significantly affect the autophagy process. This demonstrates their superiority over cisplatin, which can stimulate cancer cell survival through its effect on stimulation of autophagy.

Keywords

Thiosemicarbazones

Platinum(II)

Breast cancer

Anticancer drugs

Apoptosis

Autophagy

© 2024 The Author(s). Published by Elsevier Inc.

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