Evaluation of the impact of iPSC differentiation protocols on transcriptomic signatures

Keywords

Induced pluripotent stem cells (iPSC)

Transcriptomics

New approach methodologies (NAMs)

Characterisation

AbbreviationsaFGF

acidic Fibroblast Growth Factor

BBB

Brain capillary endothelial cells

BDNF

Brain Derived Neurotrophic Factor

BEGM

Bronchial Epithelial Cell Growth Medium

bFGF

basic Fibroblast Growth Factor

BMP4/7

Bone Morphogenetic Protein 4/7

EGF

Epidermal Growth Factor

EGM-2

Endothelial cell Growth Medium-2

ESRG

embryonic stem cell related gene

FGF9

Fibroblast Growth Factor 9

GDNF

Glial-Derived Neurotrophic Factor

GFP

green fluorescent protein

HAPLN1

hyaluronan and proteoglycan link protein 1

hCG

human chorionic gonadotropin

hESFM

human Endothelial Serum Free Medium

HGF

Hepatocyte Growth Factor

IMI

Innovative Medicines Initiative

iPSC

induced pluripotent stem cells

ITS

Insulin Transferrin Selenium

LDMAA

Liver Differentiation Medium + Amino acids

LDEV

lactose dehydrogenase elevating virus

M-CSF

Macrophage-Colony Stimulating Factor

NAMs

New approach methodologies

NEAA

Non-Essential Amino Acid

PCA

Principal component analysis

RARA

retinoic acid receptor alpha

RBP1

retinol binding protein 1

ROCKi

ROCK pathway inhibitor

RPMI

Roswell Park Memorial Institute medium

TGFβ

Transforming Growth Factor Beta

ULA

Ultra-Low Adherence Plate

VEGF

Vascular Endothelial Growth Factor

VMAT2

Vesicular Monoamine Transporter 2

Wnt3a

Wingless-Type MMTV Integration Site Family Member 3A

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