One of the most important aspects in the implementation of immunogenetics in both research and routine diagnostics concerns standardization of terminology. IMGT®, the international ImMunoGeneTics information system® (https://www.imgt.org), was at the origin of immunoinformatics, a new science at the interface of immunogenetics and bioinformatics [12].

IMGT-ONTOLOGY includes a controlled vocabulary and annotation rules which are indispensable to ensure accuracy, consistency and coherence of immunogenetics data with scientific knowledge and, most particularly, in clinical applications [13]. The IMGT concepts and standards are needed more than ever in the era of immunogenetics using next generation sequencing (NGS), which has raised the major challenge of analyzing millions of rearranged IG and TR gene sequences with inherent complexity, variability and mutational capacity [14]. This challenge extends from raw data processing and V-(D)-J gene assignment and clonotype identification to meta-analysis and clonotype comparisons between different lymphoid individuals/diseases.

A major additional challenge concerns the “vocabulary” and “language” of immunogenetics in the specific field of antigen receptors. IMGT has systematically worked on this [15,16,17,18] by providing a standardized nomenclature for immunoglobulin (IG) and T cell receptor (TR) genes in human and other vertebrates that is based on the concepts of classification (locus, group, subgroup, gene, allele) of the IMGT-ONTOLOGY (‘CLASSIFICATION’ axiom) (Fig. 1), and follows as closely as possible the HUGO Gene Nomenclature Committee (HGNC) guidelines [19]. This was applied in 1988 for the human IGL and IGH loci, and in 1989 for the variable (V), joining (J) and constant (C) genes of the newly identified human TRG locus [20, 21]: gene names/symbols in capital letters, no Greek letters, no commas, no periods/full stops (hyphens are accepted, as they are for the HLA genes). IG and TR genes and alleles are not italicized in publications.

A Hierarchy of the concepts of classification and their relationships. The definition of the reciprocal relations between concepts can be read, from one concept to the other, either ascending the hierarchy (solid arrows) or descending the hierarchy (dotted arrows). B Examples of concept instances for each concept of classification. The concept instances are associated with an instance of the “Taxon” concept, and more precisely for the “Gene” and “Allele” concepts, to an instance of the “Species” concept (here, Homo sapiens). The “Locus” concept is a concept of localization (LOCALIZATION axiom). It is shown with a reciprocal relationship to the “Gene” concept. (With permission from M-P. Lefranc and G. Lefranc, LIGM, Founders and Authors of IMGT®, the international ImMunoGeneTics information system®, https://www.imgt.org).

The three first letters indicate the locus: IGH, IGK, IGL, TRA, TRB, TRG, TRD [20,21,22,23]. The fourth letter is V (for a V-GENE), D (for a D-GENE), J (for a J-GENE) or C (for a C-GENE) (except for IGH, see below). The subsequent number(s) and/or letter(s) allow, if necessary, unambiguous identification of the gene, a single number or letter being used whenever possible: IGKC, TRGV9, TRGVA, TRAJ4 etc. For the IGH locus, the constant genes are designated by the letter (and number suffix) corresponding to the encoded isotype (IGHM, IGHD, IGHG3,…), instead of using the letter C. For the more complex situation where genes have not yet been localized and have to be named according to their subgroup (for V) or cluster (for D and J), a temporary designation is used in which the Arabic number (for the subgroup or cluster) is followed by the letter S (for subgroup or sequential, respectively), followed by the number of the gene in the subgroup or cluster. Orphon genes (OR) located outside the main chromosomal locus are designated by the IMGT four letter gene designation, followed by a number for the subgroup (if known) or between parentheses a Roman numeral for the clan, a slash, “OR”, the chromosome number (if known), a hyphen and a specific gene number and/or letter. Examples: IGKV2/OR2-1, IGHD1/OR15-1a, IGKV1/OR-1, IGHV(III)/OR16-25.

IMGT gene names (or symbols) and IMGT gene definitions (or full names) for all the human IG and TR genes were approved by the HGNC in 1999 and listed originally in the Genome DataBase (GDB), and at the NCBI’s LocusLink, then Entrez Gene, and now Gene (at www.ncbi.nlm.nih.gov/gene), as well as in the HGNC’s database at www.genenames.org. Note that in the HGNC symbols for IG and TR genes the slashes and parentheses are omitted. Otherwise, the IG and TR gene names/symbols are identical between IMGT and HGNC. All the IMGT gene definitions (including those with slashes and parentheses) have been endorsed as official gene names by HGNC (“approved name”) and NCBI Gene (“official full name”).

Under normal circumstances IG and TR names/symbols are not italicized. The single tolerated exception to these consensus rules concerns the use of italics for the names/symbols of IG and TR loci participating in chromosomal rearrangements, described in specialist databases and/or journals following the IMGT and HGNC recommendations. The use of italics indicates that an IG or TR locus is ‘translocated’, i.e affected by an internal or external (beyond the locus 5’ or 3’ border) breakpoint, and therefore does not represent the normal IG or TR locus [1, 2]. This unique exception for IG and TR loci was agreed between IMGT and HGNC following the publication of the HGNC’s recommendation to use a double colon (::) as the separator between gene symbols in translocations generating fusion genes [24]. Subsequently, the 5th edition of the WHO classification of hematological neoplasms (WHO-HAEM5) has adopted this separator for translocations which involve conventional genes and IG and TR loci in hematological neoplasms, also italicizing these genes and IG and TR loci (e.g IGH::MYC and IGH::CCND1) [25]. While the WHO-HAEM5 “follows the HGNC guidelines regarding italicizing immunoglobulin (IGH, IGK, IGL) and T cell receptor (TRA, TRB, TRD, TRG) loci”, note that this italicization is an exception limited only to ‘translocated’ IG or TR loci and only in this context of IMGT and HGNC recommendations.