Available online 17 April 2024, 101940

Tanycytes express the G6Pase system.

Tanycytes sequester G6P in the ER.

Tanycytes release glucose in hypoglycaemia through GLUTs.

Tanycytes-expressing G6Pase system regulate the energy balance.

The liver releases glucose into the blood using the glucose-6-phosphatase (G6Pase) system, a multiprotein complex located in the endoplasmic reticulum (ER). Here, we show for the first time that the G6Pase system is also expressed in hypothalamic tanycytes, and t is required to regulate energy balance.

Using automatized qRT-PCR and immunohistochemical analyses, we evaluated the expression of the G6Pase system. Fluorescent glucose analogue (2-NBDG) uptake was evaluated by 4D live-cell microscopy. Glucose release was tested using a glucose detection kit and high-content live-cell analysis instrument, Incucyte s3. In vivo G6pt knockdown in tanycytes was performed by AAV1-shG6PT-mCherry intracerebroventricular injection. Body weight gain, adipose tissue weight, food intake, glucose metabolism, c-Fos, and neuropeptide expression were evaluated at 4 weeks post-transduction.

Tanycytes sequester glucose-6-phosphate (G6P) into the ER through the G6Pase system and release glucose in hypoglycaemia via facilitative glucose transporters (GLUTs). Strikingly, in vivo tanycytic G6pt knockdown has a powerful peripheral anabolic effect observed through decreased body weight, white adipose tissue (WAT) tissue mass, and strong downregulation of lipogenesis genes. Selective deletion of G6pt in tanycytes also decreases food intake, c-Fos expression in the arcuate nucleus (ARC), and Npy mRNA expression in fasted mice.

The tanycyte-associated G6Pase system is a central mechanism involved in controlling metabolism and energy balance.

Tanycytes

G6Pase system

hypoglycaemia

energy balance

glucose-6-phosphate transporter

glucose-6-phosphatase

glucose transporter 1

glucose transporter 6

glucose transporter 2

© 2024 Published by Elsevier GmbH.