Cross Clinical Reactivity among Wild Privet and Gaint Hazel Pollinosis

Background: Pollen from various tree and grass species is the most common cause of allergy responses around
the world. Objectives: In this study, we focused on the allergenic potential and cross-reactivity between pollen
extracts of Ligustrum robustum and Corylus maxima, aiming to understand the clinical significance of these
interactions. Several proteins found in Ligustrum pollen have previously been identified as allergens that
cause sensitization and the onset of allergic symptoms in atopic people. Our study includes the cross-reactivity
between pollen extracts of L. robustum with C. maxima for clinical significance. Methods: Immunological and
biochemical characterization was carried out for the purified GST compound which was assessed by sodium
dodecyl sulfate polyacrylamide gel electrophoresis. The cross-reactivity studies among allergens and relevant
species were evaluated by enzyme-linked immunosorbent assay inhibition assays. Results: In biochemical assays,
central goods and services tax (c-GST) was not enzymatically active, whereas l-GST demonstrated high enzymatic
activity. Conclusion: This research contributes to a better understanding of the allergenic properties and crossreactivity
patterns between these two pollen sources. The obtained information could have implications for the
development of diagnostic and therapeutic strategies for individuals with allergies.

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