Fasciolosis is one of the most important liver diseases caused by the trematode Fasciola hepatica and is one of the zoonoses classified within neglected liver diseases worldwide. Within the field of veterinary medicine, it causes economic losses estimated at millions of dollars (Mehmood et al., 2017; Fairweather et al., 2020). In Mexico, an annual economic loss of US $4,239,667 has been estimated (Rodríguez-Vivas et al., 2017).

Over the years, this disease has spread due to the growth of the livestock industry throughout the world and the change in climatic factors that have allowed for the adaptation and greater geographical distribution of the intermediate host (Mas-Coma et al., 2005; Rojo et al., 2012).

For decades, the main method of control of this disease has been chemotherapy; unfortunately, the indiscriminate use of these drugs in conjunction with poor prevention and inappropriate diagnoses has allowed for the generation and increase in anthelmintic resistance worldwide (Moll et al., 2000; Ortiz et al., 2013; Ceballos et al., 2010; Olaechea et al., 2011; Kaplan and Vidyashankar, 2012; Fairweather et al., 2020; Fernández et al., 2015). Furthermore, genetic adaptations by the parasite have been identified that have fostered an increase in anthelmintic resistance (Fairweather et al., 2020).

An alternative solution to this problem is herbal medicine or the use of plant extracts with a parasiticidal effect, including Artemisia ludoviciana Nutt. spp Mexicana, which is a plant of the Asteraceae family that is located extending from Canada to Guatemala and is able to adapt to a great variety of terrains (BDMTM/UNAM, 2009). Its use is mainly medicinal for treating gastrointestinal conditions and respiratory problems, as well as for antispasmodic and dewormer treatments in humans (Andrade, 2009; BDMTM/UNAM, 2009; Juárez et al., 2013). In previous studies against F. hepatica, A. ludoviciana has shown promising in vitro fasciolicidal efficacy at different concentrations (Álvarez et al., 2015; Ibarra et al., 2012a; Ezeta et al., 2020).

Considering the possible applications of A. ludoviciana as an integral option for the treatment of fasciolosis, the objective of this study was to evaluate the toxicity of an extract in CD-1 mice.