Available online 27 December 2022, 102682
Author links open overlay panelAbstractThree strictly anaerobic strains of Escherichia coli were misidentified as Fusobacterium mortiferum, due to a deletion of the hemB gene which is involved in anaerobic respiration. An unusual antimicrobial susceptibility pattern sparked the further diagnostic strategies that eventually identified these strains as true anaerobic E. coli This phenomenon is more common than appreciated and can have an impact on clinical practice including persistent and relapsing infections.
Section snippetsResultsThe three variant Fusobacterium strains (anaerobic E. coli) were re-identified using 16S rDNA sequencing and found to match Escherichia coli with a 99.9% confidence level. The anaerobic strains were compared with their aerobic counterparts isolated from the same specimen by whole genome sequencing. After mapping to the K12 reference strain (ATCC 47076), single nucleotide polymorphism (SNP) calling identified 124,210 sites which defined the maximum likelihood phylogeny (Fig. 1). The SNPs
Declaration of competing interestAll authors had no conflict of interest.
There was no funding for this study.
AcknowledgementsAll authors had no conflict of interests. There was no funding for this study.
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