Introduction

This study aimed to determine the causes of disruption of the three-dimensional architecture of endometrial glands prepared using BD SurePath™- Liquid based cytology (SP-LBC) reagents. These reagents are presented as a sample preparation method for endometrial cytology and how this three-dimensional architecture could be retained.

Methods

The following three types of SP-LBC specimens were prepared: 1) specimens were prepared using the BD PrepMateTM (PrepMate) System after cellular fixation for 1 to 6 h (method A), 2) specimens were prepared without using the PrepMate System after cellular fixation for 1 to 6 h (method B), and 3) specimens were prepared using the PrepMate System after cellular fixation for at least 18 h (method C). Size and number of endometrial cell clusters and number of solitary scattered cells were then evaluated.

Results

Method C (71.3±57.2) yielded significantly higher numbers of cell clusters with a major axis of 200 μm or more than methods A (9.3±5.9, p < 0.001) or B (44.3±28.8, p < 0.05). Method B yielded significantly higher values than method A (p < 0.001). Method A (132.2±107.7, p < 0.001) yielded significantly higher numbers of solitary scattered cells than methods B (29.1±14.8) and C (35.7±23.3). No significant difference was found between methods B and C.

Conclusions

Retention of endometrial glandular architecture is rendered possible by allowing endometrial sample fixation times of 18 h or more, and preparing specimens on the following day.