New Findings

Our study evaluated the effects of diabetes and exercise on LPS-induced ALI. By providing a comprehensive analysis of redox status, blood-gases and histological parameters, we aimed to contribute to the ongoing debate in the literature. We demonstrated the preventive effect of exercise, but diabetes did not alter the severity of ALI.

Abstract Background and objective

: Acute lung injury (ALI) is a life-threatening respiratory condition. Diabetes (DM) is a metabolic disease, which is characterized by hyperglycemia. There is an ongoing debate on if there is a protective effect of diabetes in ALI. Exercise is a special type of physical activity that has numerous beneficial effects. Our study aims to investigate the effects of diabetes and exercise on the prognosis of ALI.

Methods

: Male, Wistar albino rats were divided into two groups (sedentary&exercise). Both groups were divided into four subgroups: Control, ALI, DM, DM+ALI (n = 6 each).Diabetes was induced by Streptozotocin (STZ) injection (50mg/kg). Maximal exercise capacity (MEC) was determined with the incremental load test. Animals were exercised on the treadmill for 45 minutes on 70% of the MEC, five days a week for 12 weeks. ALI was induced by intratracheal Lipopolysaccharide(LPS) injection (100μg/100g) 24 hours before sacrification. We made an arterial blood-gas analysis. Redox status was measured both in plasma and lung tissue. MDA and 8OHdG levels were measured in lung tissue. Histological evaluation of lung tissue has been made.

Results

: ALI caused significant damage in lung tissue, which was verified histologically, also an increase in oxidative stress parameters. Exercise prevented the lung damage done by ALI and reduced the oxidative stress in lung tissue. Diabetes did not alter the magnitude of damage done by ALI.

Conclusion

: The exercise showed a protective effect against diabetes and acute lung injury in rats. The effect of diabetes was insignificant on the prognosis of ALI.

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