Ultrasonography, a non-invasive and useful technique, is used for the examination of Atlanto-occipital space structural visualization. The collection of cerebrospinal fluid is more accurate and easier under ultrasound-guided procedure. In this study, longitudinal and transverse views of the Atlanto-occipital space were scanned and their different structural dimensions were measured in sixty healthy Beetal goats. In longitudinal plane, gap between skin and arachnoidea ranged from 8.71 to 10.21 mm (mean ± SD, 9.76 ± 0.44 mm). Depth of the subarachnoid gap dorsal and ventral to the spinal cord ranged from 2.14 to 3.23 mm (mean ± SD, 2.81 ± 0.33mm) and from 6.09 to 7.68 (mean ± SD, 7.02 ± 0.45 mm) respectively. Spinal cord diameter varied from 3.76 to 5.26 mm (mean ± SD, 4.57 ± 0.44 mm) and entire dural sac diameter varied from 12.59 to 15.69 mm (mean ± SD, 14.37 ± 0.74 mm). The spinal cord can be seen only in longitudinal plane over a distance of 1.81 to 2.93 mm (mean ± SD, 2.46 ± 0.35 mm). While in the transverse plane, gap between the skin and arachnoidea ranged from 11.01 to 13.11 mm (mean ± SD, 12.39 ± 0.54 mm). Depth of the subarachnoid space dorsal and ventral to spinal cord varied from 5.05 to 6.13 mm (mean ± SD, 5.59 ± 0.34 mm) and 4.12 to 5.25 (mean ± SD, 4.65 ± 0.29 mm) respectively. Spinal cord diameter ranged from 4.45 to 5.90 mm (mean ± SD, 5.24 ± 0.44 mm) and entire dural sac diameter varied from 14.68 to 16.96 mm (mean ± SD, 15.58 ± 0.57 mm). These standard measurements will be the reference values in healthy Beetal goats. Cerebrospinal fluid was colourless with the quantity of 2–4 ml (mean ± SD, 3 ± 0.89 ml). It was neither turbid nor coagulate. The white blood cell count was 10/µl and red blood cells were not present. Furthermore, total protein and glucose were also measured, which ranged from 23.5 to 28 mg/dl (mean ± SD, 25.78 ± 2.32 mg/dl) and 38–50 mg/dl (mean ± SD, 43.33 ± 4.60 mg/dl) respectively. Ziehl–Neelsen Staining and gram staining were negative.