L. Rachmadi, F. Hasan, M. Linggodigdo, Y. Dwina, R. Cahyanur, M. Adham

Department of Anatomical Pathology, Faculty of Medicine, Universitas Indonesia, Dr. Cipo Mangunkusumo General Hospital, Jakarta, Indonesia. lisnawatidrsppa@gmail.com

OBJECTIVE: Nasopharyngeal carcinoma (NPC) is a prevalent malignancy in Southeast Asia and Southern China, with a notable incidence in Indonesia. This study aimed to characterize the expression and correlation of Yes-associated protein (YAP1) and miR-340-5p in NPC metastasis tissues.

MATERIALS AND METHODS: This study utilized clinical samples from primary tumors of NPC patients to investigate the expression of YAP1 and miR-340-5p. The Cancer Genome Atlas (TCGA) Head and Neck Cancer dataset was analyzed to assess YAP1 and miR-340-5p expression in broader head and neck cancer samples. Protein-protein interaction (PPI) and functional enrichment analyses were performed to understand the putative regulatory mechanisms of YAP1 and miR-340-5p head and neck cancer. YAP1 mRNA and miR-340-5p level expression were measured by reverse transcription-quantitative polymerase chain reaction (RT-qPCR), and statistical analyses were performed to compare the expression of these markers in NPC samples.

RESULTS: Analysis of clinical samples revealed lower expression levels of YAP1 and miR-340-5p in metastasis NPC cases compared to non-metastatic cases (p<0.0001). YAP1 and miR-340-5p revealed a negative correlation in metastasis and non-metastasis samples but were statistically insignificant. Additionally, both genes showed significantly lower expression in stage IVB compared to stage II, III, and IVA NPC tissues (p<0.0001). The TCGA dataset showed consistent decreases in YAP1 and miR-340-5p expression in head and neck cancer tumors as opposed to normal tissues. Functional enrichment and PPI analysis suggested the involvement of the Hippo signaling pathway and other cancer-related pathways in NPC progression.

CONCLUSIONS: The study highlights the under-expressed YAP1 and miR-340-5p in metastasis tumor cases, suggesting their potential role as a tumor suppressor in NPC.

This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License

L. Rachmadi, F. Hasan, M. Linggodigdo, Y. Dwina, R. Cahyanur, M. Adham
Unveiling the interplay of YAP1-driven pathways and miR-340-5P expression: insights into nasopharyngeal cancer metastasis

Eur Rev Med Pharmacol Sci
Year: 2024
Vol. 28 - N. 22
Pages: 4576-4590
DOI: 10.26355/eurrev_202411_36954