Eur Rev Med Pharmacol Sci 2024; 28 (21): 4546-4552
DOI: 10.26355/eurrev_202411_36913

E. Uzunhisarcıklı

Department of Pharmacology, Faculty of Pharmacy, Erciyes University, Kayseri, Turkey. ebruozturk@erciyes.edu.tr

OBJECTIVE: This study aimed to investigate the effect of psoralidin, a natural phenolic coumarin compound, on MK-801-induced neurotoxicity that may cause Alzheimer’s disease and to determine the phosphodiesterase (PDE)-related molecular mechanism of action.

MATERIALS AND METHODS: In this study, neurotoxicity was performed using the MK-801 in the HT-22 cell line. The effects of compounds on the proliferation of HT-22 cells were determined by Real-Time Cell Analysis (RTCA). After measuring the total protein concentration, the PDE4A protein level was determined using the Western blot method.

RESULTS: Psoralidin (100, 200, 400 µM) has been shown to have a neuroprotective effect against MK-801-induced neurotoxicity, as indicated by Real-Time Cell Analysis. In HT-22 cells, the half maximal effective concentration (EC50) value of psoralidin was calculated to be 230.4 µM, IC50 value of MK-801 was calculated to be 62.4 µM at 24 hours. It has been determined that psoralidin (200, 400 µM) inhibits PDE4A by using the Western blot method.

CONCLUSIONS: This research uncovers that psoralidin has neuroprotective effects in MK801-associated accumulation of the excitatory amino acid glutamate neurodegeneration and Alzheimer’s disease.

This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License

E. Uzunhisarcıklı
Potential PDE4A inhibition-mediated neuroprotective effects of psoralidin

Eur Rev Med Pharmacol Sci
Year: 2024
Vol. 28 - N. 21
Pages: 4546-4552
DOI: 10.26355/eurrev_202411_36913