Tim Czopka1, Kelly Monk2 and Francesca Peri3 1Centre for Clinical Brain Sciences, The University of Edinburgh, Edinburgh EH16 4SB, United Kingdom 2Vollum Institute, Oregon Health and Science University, Portland, Oregon 97239, USA 3Department of Molecular Life Sciences, University of Zürich, 8057 Zürich, Switzerland Correspondence: Tim.Czopkaed.ac.uk; monkohsu.edu; Francesca.periuzh.ch

Over the past decades the zebrafish has emerged as an excellent model organism with which to study the biology of all glial cell types in nervous system development, plasticity, and regeneration. In this review, which builds on the earlier work by Lyons and Talbot in 2015, we will summarize how the relative ease to manipulate the zebrafish genome and its suitability for intravital imaging have helped understand principles of glial cell biology with a focus on oligodendrocytes, microglia, and astrocytes. We will highlight recent findings on the diverse properties and functions of these glial cell types in the central nervous system and discuss open questions and future directions of the field.