Lu Xu,1,* Jing Liu,2,* Suli Li,1 Xingchen Lu,1 Wenjie Gu,1 Shunhua Zhu,1 Meng Wang,1 Xiaojin Wu,3 Qingli Huang1
1Public Experimental Research Center of Xuzhou Medical University, Xuzhou, Jiangsu Province, 221004, People’s Republic of China; 2Department of Neurology, the Affiliated Xuzhou Municipal Hospital of Xuzhou Medical University, Xuzhou No. 1 People’s Hospital, Xuzhou, Jiangsu Province, 221100, People’s Republic of China; 3Department of radiotherapy, the affiliated Xuzhou Municipal Hospital of Xuzhou Medical University, Xuzhou No. 1 People’s Hospital, Xuzhou, Jiangsu Province, 221100, People’s Republic of China
Purpose: Developing novel multimodal nanomaterials-based anticancer agents to meet complex clinical demands is an urgent challenge. This study presents a novel uniform hollow S-doped NiCuFe Prussian blue analogue (NiCuFe-S) with satisfactory size and properties as anticancer agents for efficient cervical cancer therapy using a simple and environmentally friendly procedure.
Methods: The formation mechanism and the reason for enhanced performance of NiCuFe-S were characterized and discussed by diverse spectroscopic and microscopic methods. Moreover, to demonstrate the anti-cancer ability of NiCuFe-S, in vitro and in vivo experiments were carried out.
Results: Compared to the non-doped NiCuFe, the NiCuFe-S exhibited significantly enhanced photothermal and catalytic activity attributed to the electronic bandgap-narrowing effect and the increased electron circuit paths resulting from S doping. The hollow structure of NiCuFe-S facilitated the loading of small-molecule drugs, such as doxorubicin (DOX), transforming it into a multimodal nanoplatform for cervical cancer treatment. In vitro and in vivo experiments proved the potential of the NiCuFe-S nanotheranostic agent for chemodynamic therapy (CDT), photothermal therapy (PTT), and chemotherapy for cervical cancer.
Conclusion: This research not only overcomes inherent limitations but also significantly broadens the applications of Prussian blue analogues in biomedicine.
Keywords: nanomaterials, Prussian blue analogue, catalytic, photothermal therapy, anticancer
With the development of nanotechnology, various nanomaterials-based cancer therapy strategies including photothermal therapy (PTT), chemodynamic therapy (CDT) and other novel methods were developed and have arisen as research hotspots for researchers, emphasizing the importance of nanoparticles endowed with photothermal or catalytic properties in achieving successful treatment outcomes.1–10 PTT is a novel noninvasive cancer treatment, which can switch light energy into hyperthermia at tumor site by employing site-specific nanoparticles with photothermal conversion performance.1,3,4,11,12 In this way, PTT could induce apoptosis and/or necrosis of cancer cells and achieve ideal curative effect. CDT is defined as an in-situ treatment with little side effects, which could kill cancer cells effectively by highly toxic hydroxyl radical (⋅OH) using metal-related to Fenton reaction or Fenton-like reaction in mildly acidic conditions of cancer.2,5,6,13–16 And, many studies have proved the synergistic anti-tumor effect of PTT combined with CDT, which achieved superior outcomes than the single mode of PTT and CDT.17–20 This combination therapy not only could synchronously kill cancer cells by hyperthermia of PTT and reactive oxygen species (ROS) of CDT to enhance the efficiency of cancer therapy but also enhance the production of ROS by PTT during the period of therapy.17–20
Various nanostructures, including noble metal nanomaterials, inorganic nanoparticles, polymer nanocomposites, and organic nanostructures, have been successfully designed and fabricated to enhance photothermal or catalytic properties, thereby improving the efficiency of cancer treatment.1–20 Among them, traditional transition metal-based nanomaterials including metals oxides, metals sulfides and metals hydroxides of Ni, Cu, Fe, Mn have attracted numerous attentions for anticancer due to their outstanding photothermal or catalytic properties, and facile preparation.21–23 However, poor biocompatibility and small specific surface area of traditional nanomaterials often limited their further application. Fortunately, when integrated them into a metal-organic frameworks (MOFs) with desired size (<100nm), it will possess large surface area, porosity, and good biocompatibility, which is expected as a potential candidate for application for cancer therapy.24–29 Metal-organic frameworks (MOFs), with their unique framework featuring high porosity, large specific surface area, and adjustable metal nodes or organic ligands, have garnered substantial attention for their versatility in energy, biomedicine, catalysis, and other applications.24–29 As a subclass of MOFs, Prussian blue analogues (PBAs) have elicited substantial interest and explored as platforms for diagnosis and treatment for various diseases due to lower cost, simpler synthesis, and more adjustable catalytic activity.27–35 However, there are limited reports on the cancer therapy application of PBAs, primarily due to their poor NIR absorption and low photothermal conversion efficiency. Enhancing the photothermal efficiency of PBAs and developing PBA-based anticancer agents for efficient treatment for cancer is significant to meet complex clinical demands. Heteroatom doping has been proposed as a viable approach to enhance the inherent properties of nanomaterials by regulating their electronic structure.36–40 For instance, Zhu et al reported enhanced interfacial electron transfer and improved catalytic activity in (g-C3N4)/MS2 (M=Sn, Zr) heterojunctions through O, S doping.39 The difference in charge density indicated that interfacial electron transfer was increased by O and S doping, thus further improving the catalytic activity of nanocomposites. Su et al developed a sulfur (S)-doped (Ti−S−TiO2−x) nanocomposite with outstanding photothermal conversion efficiency, attributing it to the introduction of oxygen deficiency by S dopants, which improved NIR absorption and the efficiency of electron–hole separation.40
Herein, we prepared a novel uniform hollow S-doped NiCuFe Prussian blue analogue (NiCuFe-S) with satisfactory size (<100nm) and properties as anticancer agents for cervical cancer using a simple and environmentally friendly procedure. As illustrated in Scheme 1, hollow S-doped NiCuFe PBAs were synthesized using a facile method. Our study revealed superior photothermal conversion performance and catalytic activity in S-doped PBAs compared to non-doped PBAs, a topic that has been largely understudied. Additionally, the formation mechanism of NiCuFe-S PBAs and the reason on their enhanced performance were comprehensively investigated. Vitro and vivo experiments proved that the prepared hollow S-doped NiCuFe PBAs could serve as a novel nanoplatform for enhanced photothermal and ROS anticancer therapy against cervical cancer. This research proved the feasibility of tumor therapy using S-doped PBA as anticancer agents and significantly expanded the potential of their applications in biomedicine.
Scheme 1 The illustration of construction of S doped hollow multi-metallic NiCuFe-S nanoplatform and its application for enhanced anticancer for cervical cancer.
Experimental Section Materials, Characterization and PerformanceAll chemicals and characterization instruments were all listed in supplementary materials (S1 and S2). The photothermal and catalytic performance of hollow NiCuFe-S were evaluated using NIR laser irradiation and 3,3′,5,5′-tetramethylbenzidine (TMB) following early procedures (S3).
Synthesis of NiCuFe-SInitially, NiCuFe-PBA nanocubes were synthesized following a modified procedure41 (S4). Subsequently, 10 mL of 2.8 mg/mL sodium sulfide aqueous solution was added to the 20 mL NiCuFe-PBA ethanol suspensions (0.5 mg/mL) with stirring. The mixed solution was then placed into an autoclave and maintained at 100°C for 6 h. The resulting hollow S-doped NiCuFe PBA was obtained through centrifugation and washing, denoted as NiCuFe-S. To enhance biocompatibility and enable chemotherapy, small-molecule PEG and chemotherapy drug DOX were modified and loaded onto NiCuFe-S, resulting in P-NiCuFe-S and DP-NiCuFe-S, respectively. Typically, PEG was added to 20 mL of deionized water at a mass ratio of 1:1 with NiCuFe-S and stirred at room temperature for 6 h. After the precipitate was collected by centrifugation and then added to tris solution with the same mass of DOX and stirred for 6 h. The precipitates were collected by centrifugation.
In vitro Anti-Cancer Experiments of Hollow NiCuFe-SInitially, the biocompatibility and biosafety of the prepared hollow NiCuFe-S were evaluated in vitro using the normal cell line (NRK-52E) with the traditional Cell Counting Kit (CCK8) method. Subsequently, human cervical cancer Hela cells were co-incubated with the hollow NiCuFe-S nanoplatform to investigate the in vitro anti-cancer performance of NiCuFe-S, employing the CCK8 assay. All cells were purchased from the Procell Life Science & Technology Co (Wuhan, China). More details were shown in S5.
To visually confirm the anti-cancer effect of NiCuFe-S, Calcein AM (green fluorescence)/propidium iodide (PI, red fluorescence) live/dead stain experiments were conducted. Hela cells (1 × 104 cells) were seeded into 96-well plates and incubated in an incubator for 12 hours. After treated with P-NiCuFe-S and DP-NiCuFe-S of 100 μg/mL in different groups for another 4 hours, Calcein-AM/PI (KeyGen) solution (100 μL) for staining live/dead cells was used to replace the supernatant in 96 well plate. The experiments were observed with different colors under the inverted fluorescence microscope (Olympus IX73). Intracellular reactive oxygen species (ROS) assay, flow cytometry assay, and Western blot experiments were also performed to confirm the anti-cancer performance of NiCuFe-S nanoplatform in vitro. The details were shown in S6.
In vivo Anti-Cancer Performance of Hollow NiCuFe-SAll experimental protocols involving animals were carried out with permission from the Institutional Animal Care and Use Committee (IACUC) of Xuzhou Medical University (permission NO.202309T022 in 2023), and the procedures were as per the regulations and guidelines of IACUC (Guidelines for ethical review of experimental animal welfare in Xuzhou Medical University). The animal tumor models were established using balb/c nude female mice (6 weeks old, GemPharmatech LLC, Jiangsu, China) by injecting Hela cells (6 × 105). Once tumors reached a certain size (approximately 100 mm3), mice were injected through the tail vein on days 2 and 10 with modified and drug-loaded NiCuFe-S (P-NiCuFe-S and DP-NiCuFe-S). Mice were divided into six groups (n = 5 per group) based on different treatment programs: control (injected with PBS), control+NIR (injected with PBS and laser irradiation (1064 nm, 1.0 W/cm−2, 5 minutes)), P-NiCuFe-S (injected with modified NiCuFe-S), P-NiCuFe-S+NIR (injected with modified NiCuFe-S and laser irradiation (1064 nm, 1.0 W/cm−2, 5 minutes)), DP-NiCuFe-S (injected with drug-loaded NiCuFe-S), and DP-NiCuFe-S+NIR (injected with drug-loaded NiCuFe-S and laser irradiation (1064 nm, 1.0 W/cm−2, 5 minutes)). During the process of laser irradiation, real-time temperature changes at the tumor site were monitored using an infrared thermal imager, and infrared thermal imaging images of the tumor site were recorded at 1, 2, 3, 4, and 5 minutes. The weights and tumor sizes of mice were monitored every two days for 16 days, and weight change curves and volume changes for each group were plotted according to experimental records. Finally, the mice were euthanized after 16 days, and the tumor tissue and main organs were harvested for H&E staining. Additionally, analyses for blood routine parameters and blood biochemistry were performed using blood samples taken through the retroorbital plexus region of mice in each group after anti-cancer treatment. The blood samples were left at room temperature for 2 h and centrifuged at 4°C with 3000 rpm for 15 min, and then the supernatant was taken for biochemical detection. The blood samples extracting in vacutainer (containing 3.2% sodium citrate anticoagulant) were taken 200 μL for the blood routine parameters.
Results and Discussion CharacterizationTransmission electron microscopy (TEM) and scanning electron microscopy (SEM) were utilized to examine the morphological evolution of the prepared samples. As depicted in Figure 1a, uniform NiCuFe nanocubes with an average size of approximately 50 nm were obtained through a modified precipitation method. Subsequently, these nanocubes were subjected to treatment with Na2S, resulting in the formation of hollow S-doped NiCuFe, as illustrated in Figure 1b. SEM images (Figure 1c) revealed the presence of holes, providing further confirmation of the hollow structure after hydrothermal treatment with Na2S. Energy dispersive X-ray (EDX) spectrum analysis was performed to investigate the elemental compositions of hollow S-doped NiCuFe in Figure 1d. The presence of six elements, namely C, N, S, Ni, Fe, and Cu, was confirmed, validating successful S doping. These elements were found to be homogeneously distributed in all hollow cubes, as indicated by EDX mapping images (Figure 1e–k), validating the uniform distribution of S dopants. X-ray diffraction (XRD) was employed to examine variations in the crystal structure (Figure 1l). Typical peaks at 17.3, 24.6, 35.1, and 39.4° were observed, consistent with the early report on NiCuFe.41 Importantly, after doping with S2-, no substantial change was found in the XRD pattern, indicating that the addition of S2- did not affect the crystal structure. X-ray photoelectron spectroscopy (XPS) experiments also prove this result. In Figure 1m and n, we can find that low valence of Fe2+ and Cu+ become dominant valence state in NiCuFe-S after S doping, which will enhance the Fenton-like catalytic activity of the PBAs.
Figure 1 TEM images of (a) NiCuFe (b) NiCuFe-S (c) the SEM images of NiCuFe-S (d) EDS spectrum and (e) the HAADF images and (f–k) EDS mapping NiCuFe-S (l) XRD (m and n) XPS spectra of Fe 2p and Cu 2p in NiCuFe and NiCuFe-S.
Performance TestingWe found strong UV–vis absorption of NiCuFe from 600 nm to 1100 nm after S doping in Figure S1, which will benefit to its photothermal performance. In Figure 2, the photothermal properties of NiCuFe-S were investigated. The photothermal-induced temperatures of NiCuFe-S were significantly higher than those of NiCuFe and water, as depicted in Figure 2a and b. The temperature of the NiCuFe-S aqueous suspension rose rapidly to 45.7 °C in 5 min under 808 nm laser radiation (1.5 W/cm2), resulting in an approximately 18.7 °C temperature increase. In contrast, only temperature increases of 1.2°C and 2.1°C were observed in water and NiCuFe aqueous suspension under the same conditions (Figure 2a and b). Moreover, an apparent concentration-dependent and laser power-dependent photothermal effect was observed (Figure 2c and Figure S2). When the concentration of NiCuFe-S aqueous suspension increased to 500 µg/mL, a 33.3°C temperature change was observed, indicative of a typical state of hyperthermia. As Figure 2d shows, photothermal stability experiments were conducted by monitoring the temperature variation every 30 minutes during laser on/off irradiation, repeated five times. The maximum temperature of each cycle remained almost identical, demonstrating the good photo and thermal stability of the hollow NiCuFe-S. The photothermal conversion efficiency was also calculated according to early methods in Figure 2e and 2f which is approximately 52.1% and superior than many other PB-based nanomaterials reported in previous literatures.42–45 According to earlier reports highlighting the desirable deeper tissue penetration ability of the second NIR (NIR-II) light compared to the first NIR (NIR-I) laser,46,47 the photothermal performance of NiCuFe-S in the second NIR (NIR-II) bio-windows (1064 nm) was also evaluated. The same trend was observed, as depicted in Figure 2g–i. All experimental results indicated that NiCuFe-S has the valuable potential to enhance photothermal therapy in both NIR-I and NIR-II dual windows.
Figure 2 (a) Thermal infrared images of H2O and the as-prepared nanomaterials (NiCuFe and NiCuFe-S) solutions (200 μg/mL) under 808 nm NIR laser irradiation (1.5 W/cm2) for 5 min and (b) the corresponding heating curves (c) heating curves of NiCuFe-S solutions with different concentrations (d) The photothermal stability of NiCuFe-S dispersion (200 μg/mL) by five cycles of laser on/off irradiation under 808 nm laser irradiation (1.5 W/cm2) (e) The course of the temperature of the NiCuFe-S dispersion (200 μg/mL) under 808 nm laser irradiation (1.5 W/cm2) to the highest point, followed by cooling to room temperature after turning off the laser, respectively (f) The linear relationship of time data versus −ln θ obtained from the cooling period of picture under 808 nm respectively (g) Heating curves of H2O and the as-prepared nanomaterials (NiCuFe and NiCuFe-S) solutions (200 μg/mL) under 1064 nm NIR laser irradiation (1.0 W/cm2) for 5 min (h) the corresponding heating curves (i) The photothermal stability of NiCuFe-S dispersion (200 μg/mL) by five cycles of laser on/off irradiation under 1064 nm laser irradiation (1.0W/cm2).
PBAs exhibit outstanding catalytic activity due to the presence of adjustable metal active sites, crucial for enhancing catalytic performance. As shown in Figure 3, peroxidase-like activity was investigated using TMB as an indicator. Compared to H2O2 and NiCuFe, the as-prepared hollow NiCuFe-S showed the highest UV–vis absorption peaks at 652 nm and 895 nm in Figure 3a, suggesting higher catalytic performance than H2O2 and NiCuFe. Furthermore, acidic conditions were found to be more suitable for enzyme-like catalytic reactions than a neutral environment (Figure 3b), aligning with the acidic nature of the tumor microenvironment, making it more conducive to target cancer. Additionally, even at a reduced concentration of 1 µg/mL, significant peaks were observed (Figure 3c), indicating the high efficiency of the catalytic performance of NiCuFe-S. The typical Michaelis–Menten model was used to investigate the apparent steady-state kinetic parameters (Km). The Km values were 0.838 mM by changing the concentration of TMB in Figure 3d and e, comparable to Fe-Ag2S (0.821 mM) and MoS2@MgFe2O4 (0.806 mM) nanozymes in earlier literature.48,49 These results indicate that the as-prepared hollow NiCuFe-S exhibited excellent peroxidase-like activity. ESR spectroscopy with DMPO as a spin-trapping agent was also conducted (Figure 3f). Higher ESR signals were detected in NiCuFe-S than in H2O2 and NiCuFe, indicating the generation of more ·OH by NiCuFe-S.
Figure 3 (a) Enzyme catalytic activity of the as-prepared nanomaterials (NiCuFe and NiCuFe-S) on TMB at pH 5.0 (nanomaterials, 16 μg/mL; TMB 0.1M; H2O2, 50 mM; pH 5.0) (b) Enzyme catalytic activity of NiCuFe-S on TMB at different pH (c) Enzyme catalytic activity of NiCuFe-S with different concentrations. (d) Kinetic study of NiCuFe-S against different concentration of TMB (e) The Michaelis–Menten curves of NiCuFe-S against the mentioned concentration of TMB (f) EPR spectra of H2O2, NiCuFe and NiCuFe-S.
Mechanism AnalysisThe mechanisms of the formation of the hollow structures and their outstanding performances were comprehensively investigated. The formation of hollow structures can be ascribed to the differential diffusion rates between metal ions and S2- ions. In this case, when the Na2S solution was added to the solid NiCuFe nanocubes, outward transport of metal ions and inward transport of S2- ions occurred. However, as a result of the larger size of S2- ions, they diffused more slowly than metal ions, leading to the reactions predominantly occurring in the outer layers. As a result, the valence states of some metal ions (Fe3+ and Cu2+) were adjusted, leading to the formation of a hollow structure with high photothermal and catalytic performance, as illustrated in Scheme 1.50 The semiconductor’s band structure is crucial for its efficiency and optical properties. The band gaps of NiCuFe-S (Eg values) were calculated and found to be 1.85 eV and 1.83 eV for NiCuFe and NiCuFe-S in Figure S3, respectively. The doping of heteroatoms (S atom) into the PBA lattice induced a net electronic bandgap-narrowing effect, resulting in red-shifting and moving closer to the NIR regime due to the lower bandgap energy. Moreover, the introduction of heteroatoms (S atom) increased the electron circuit paths, reducing the recombination of electron–hole pairs and promoting the catalytic activity of PBAs.
Therapeutic Properties of NiCuFe-S in vitroConsidering the outstanding photothermal and catalytic properties discussed earlier, the S-doped PBA was anticipated to replace Prussian blue as a new platform with higher performance for tumor therapy. The construction of the nanoplatform of hollow NiCuFe-S and their anti-cancer properties in vitro is demonstrated in Figure 4. Initially, biocompatible polyethylene glycol (PEG) was surface-modified on the NiCuFe-S to enhance their biosafety, a critical factor for the clinical application of nanotherapeutics.
Figure 4 (a) Absorption spectra of NiCuFe-S and DP-NiCuFe-S (b) Cell viability of normal NRK-52E cells treated with P-NiCuFe-S at different concentrations (n=3). (c) Cell viability of Hela cells treated with P-NiCuFe-S, P-NiCuFe-S+NIR, DP-NiCuFe-S, DP-NiCuFe-S-NIR for 24h, respectively (“+NIR” means with 1064 nm, 1.0W/cm2, 5min laser irradiation) (n=3). (d) Fluorescence images of calcein-AM/PI double-staining Hela cells after incubation with PBS, PBS+NIR, P-NiCuFe-S, P-NiCuFe-S+NIR, DP-NiCuFe-S, DP-NiCuFe-S+NIR, respectively.
FTIR analysis in Figure S4 revealed the successful PEG coating due to new peaks of C-O at 989 cm−1.51 Subsequently, the PBAs loaded with the cytotoxic chemotherapy drug, DOX, leveraging the porous and high specific surface properties of MOFs. Figure 4a and Figure S5 also demonstrated the presence of PEG and DOX, as evidenced by the appearance of DOX absorption at 480 nm and the significant change in Zeta potential. Furthermore, we also found that the modification of PEG and loading DOX had little affection of the morphologies and properties of NiCuFe-S in Figures S6 and S7. The DOX loading rate was also calculated, which is about 96.0%, as per the following formula, which is higher than early reports on similar nanostructures:35,36 Drug Load rate = (loaded DOX/input DOX)*100%.
As shown in Figure 4b, more than 80% of normal NRK-52E cells survived, even as the concentrations of PEG-modified NiCuFe-S increased to 200 μg/mL. This survival rate was superior to that of most PBAs reported in earlier studies.35,52 Subsequently, human cervical cancer Hela cells were selected to investigate the anticancer performance of the prepared nanoplatform (P-NiCuFe-S and DP-NiCuFe-S) in vitro (Figure 4c). Six experimental groups with different treatments were established: I: PBS (pH 7.4, 10 mM); II: PBS+1064 nm laser irradiation (1.0 W/cm2, 5 min); III: P-NiCuFe-S (0, 6.25, 12.5, 25, 50, 100, 200μg/mL); IV: P-NiCuFe-S (0, 6.25, 12.5, 25, 50, 100, 200μg/mL) +1064 nm laser irradiation (1.0 W/cm2, 5 min); V: DP-NiCuFe-S (0, 6.25, 12.5, 25, 50, 100, 200μg/mL); VI: DP-NiCuFe-S (0, 6.25, 12.5, 25, 50, 100, 200μg/mL) +1064 nm laser irradiation (1.0 W/cm2, 5 min). Observations revealed that almost no cells were killed in groups I and II. Group III (P-NiCuFe-S only) exhibited a slight killing effect on Hela cells at high concentrations (50, 100, 200 μg/mL), attributed to the outstanding catalytic activity in the acidic environment of cancer cells. In comparison, DP-NiCuFe-S in group V caused some harm to the cells, with 31.9% and 26.0% of cells killed at concentrations of 100 and 200 μg/mL, respectively. Importantly, the released DOX played a significant role in inhibiting tumor cells through chemotherapy effects, as shown in Figure S8. Similarly, cells in group IV showed approximately 50% mortality, while 50% of cancer cells died when treated with P-NiCuFe-S and laser irradiation. Excitingly, the survival rate dropped to less than 20% for Hela cells in group VI when treated with the constructed nanoplatform (DP-NiCuFe-S, 200 μg/mL) under 1064 nm laser irradiation for 5 min attributed to the synergistic effect of multi-mode treatments. Fluorescence microscope images of cells in different groups stained by AM and PI assay were acquired to distinguish between living and dead cells (Figure 4d). The highest cell mortality was observed in group VI, followed by group V, group IV, group III, group II and group I, consistent with the CCK 8 assay results. These findings further confirm the in vitro advanced therapeutic effect of hollow S-doped NiCuFe PBAs.
Mechanism of Anti-Tumor of NiCuFe-SUnderstanding the mechanisms underlying the anti-tumor properties of nanomaterials is crucial for their medical application in cancer treatment. In this context, various experiments were conducted to explore the subcellular localization of hollow cubes, intracellular ROS production, and marker protein levels in Hela cells. As shown in Figure 5a and b, typical images of cells treated with DP-NiCuFe-S revealed the presence of many hollow nanocubes in the cytoplasm, confirming efficient uptake by cancer cells. Once taken up, these hollow PBA nanocubes were triggered by the acidic tumor microenvironment (TME), characterized by excessive H+, H2O2, and GSH. On the one hand, the peroxidase-like performance of NiCuFe-S was activated by the abundant H+ and H2O2 in TME. This led to the generation of large amounts of reactive oxygen species (ROS) through a cascade of Fenton-like reactions involving transition metal ions (Cu2+/Cu+ and Fe3+/Fe2+), as demonstrated in the intracellular ROS production experiments in Figure 5c. The use of DCFH-DA as a probe converted it into dichlorofluorescein (DCF) with green fluorescence under oxidative stress in cells. The fluorescence signals observed in the cells treated with the nanocubes confirmed ROS production and NIR irradiation enhanced the signals, indicating nanocube degradation and increased •OH generation, leading to enhanced cell apoptosis. On the other hand, as the nanocubes degraded in the TME, DOX was sustainably released in the hollow section, triggering targeted chemotherapy for cancer and reducing the side effects of traditional chemotherapy. Furthermore, the prepared NiCuFe-S exhibited excellent photothermal conversion performance. The introduction of NIR laser irradiation achieved local hyperthermia at the tumor site, further enhancing ROS production and DOX release. To further elucidate the antitumor mechanism of NiCuFe-S, flow cytometry was employed to quantitatively detect apoptosis through Annexin V-FITC/PI double staining. In Figure 5d, Hela cells treated with irradiation alone showed negligible necrotic and apoptotic cells. Compared to NiCuFe-S, the percentage of apoptotic cells in NiCuFe-S treated with laser irradiation (NiCuFe-S+NIR) and DP-NiCuFe-S increased to 23.90 % and 87.44%, respectively. The DP-NiCuFe-S+NIR group exhibited the highest apoptosis rate (94.62%). Further investigation into the molecular-level impact of NiCuFe-S on the apoptosis pathway revealed a significant decrease in the expression of antiapoptotic protein Bcl-2 and a notable increase in cleaved caspase-3 levels in DP-NiCuFe-S groups in Figure 5e, suggesting that DP-NiCuFe induced tumor cell apoptosis to the greatest extent. Moreover, the photothermal effect enhanced the antitumor efficacy of these nanomaterials in vitro.
Figure 5 (a and b) TEM images of Hela cells after treatment with DP-NiCuFe-S. (c) Intracellular DCF fluorescence of Hela cells after incubation with PBS, PBS+NIR, P-NiCuFe-S, P-NiCuFe-S+NIR, DP-NiCuFe-S, DP-NiCuFe-S+NIR. (d) Flow cytometry analysis of cell apoptosis in Hela cells treated with different formulations. (e) Western blot of the protein expression of Bcl-2, caspase-3, cleaved-caspase-3 in Hela cells from different treatments.
Therapeutic Properties of NiCuFe-S in vivoThe in vivo antitumor experiments of the NiCuFe-S nanoplatform were conducted using balb/c nude female mice, as depicted in Figure 6. The tumor model was established by injecting Hela cells, and all mice underwent the treatment procedure outlined in Figure 6a. According to the treatment methods, the mice were divided into six groups (5 mice per group): I: intravenous injection of PBS (pH 7.4, 10 mM); II: intravenous injection of PBS+1064 nm laser irradiation; III: intravenous injection of P-NiCuFe-S (5 mg/kg); IV: intravenous injection of P-NiCuFe-S (5 mg/kg)+1064 nm laser irradiation (1.0 W cm−2, 5 min); V: intravenous injection of DP-NiCuFe-S (5 mg/kg); VI: intravenous injection of DP-NiCuFe-S (5 mg/kg)+1064 nm laser irradiation (1.0 W cm−2, 5 min). First, photothermal images in the laser irradiation groups (groups II, IV, and VI) were obtained to evaluate the antitumor efficacy of NiCuFe-S in vivo (Figure 6b). In comparison to the negligible temperature fluctuation of tumors in the PBS + 1064 nm laser irradiation group (II group), the temperature at the tumor sites in groups IV and VI (intravenous injection of P-NiCuFe-S (5 mg/kg) + 1064 nm laser irradiation and intravenous injection of DP-NiCuFe-S (5 mg/kg) + 1064 nm laser irradiation group) increased rapidly, demonstrating the satisfactory photothermal performance of NiCuFe-S in vivo. Next, the tumor sizes and mice weights in the different groups were recorded (Figure 6c–e). No significant change in the body weight of all mice was observed (Figure 6c), indicating negligible side effects of the nanoplatforms on normal organs. However, for tumors, despite achieving a certain therapeutic effect in groups III, IV, and V, the VI group (intravenous injection of P-NiCuFe-S (5 mg/kg) + 1064 nm laser irradiation) displayed the best tumor inhibition efficacy among all groups (Figure 6d and e), indicating the synergistic effect of PTT, chemodynamic therapy (CDT), and chemotherapy in the treatment process. To further investigate the biosafety profile of the NiCuFe-S nanoplatform, major organs (heart, liver, spleen, lung, and kidney) and tumors of the mice were harvested after 16 days of treatment, and hematoxylin and eosin (H&E) staining experiments were performed (Figure 7a). Negligible tissue damage or inflammatory lesions were observed, indicating little toxicity of NiCuFe-S in vivo. However, at tumor site, the nucleus of tumor cells was large and were pale blue in control group. For the P-NiCuFe-S+NIR group treated tumors, the cancer cells were clearly damaged and got local necrosis, indicating that the nanomaterials had superior killing ability against tumor. Additionally, no significant differences were detected in the listed blood routine indexes and blood biochemistry parameters (Figure 7b and c), confirming the good biocompatibility of the as-prepared NiCuFe-S. These results collectively demonstrate the good biosafety profile and high anti-cancer efficiency of the developed NiCuFe-S nanoplatform.
Figure 6 (a) Schematic illustration of therapeutic protocol for tumor-bearing mice (b) Typical thermal images of tumor-bearing mice under NIR irradiation after tail injection of P-NiCuFe-S and DP-NiCuFe-S (c) Animal body weight change curves. (d) Images of tumors of tumor-bearing mouse after 16 days treated with different formulations (PBS, PBS+NIR, P-NiCuFe-S, P-NiCuFe-S+NIR, DP-NiCuFe-S, and DP-NiCuFe-S+NIR). (e) The corresponding curves of the relative volume of tumors from different treatment.
Figure 7 (a) H&E-stained sections of the tumor tissue and main organs from Hela tumor-bearing mice in various groups, Scale bar, 100 μm. (b and c) Typical biochemical parameters for Hela tumor-bearing mice in various groups (notes: ALT-alanine aminotransferase; AST: aspartate aminotransferase; T-BIL-total bilirubin; ALB-albumin; WBC-white blood cells; RBC-red blood cells; PLT- platelet).
ConclusionOverall, this work successfully constructed a novel anti-cancer nanoplatform based on uniform hollow S-doped NiCuFe PBA. The introduction of S dopants significantly enhanced the photothermal conversion efficiency and enzyme-like catalytic activity of PBAs. In vitro and in vivo experiments confirmed the excellent anti-cancer performance of the S-doped PBAs. The formation and performance mechanisms were comprehensively investigated through a series of experiments. Importantly, this work overcomes the persistent problem of low photothermal conversion performance of PBAs, opening up new possibilities for their application in biomedicine.
Data Sharing StatementThe authors declare that the main data supporting the findings and conclusions of this study are available within the article. Extra data are available from the corresponding author upon request.
AcknowledgmentsThis work was financially supported by the Natural Science Foundation of Jiangsu Province of China (BK20231171) Science and Technology Innovation Project of Xuzhou City (KC22120) and Jiangsu Province Traditional Chinese medicine science and technology development program (grant No. MS2022066).
Author ContributionsAll authors made a significant contribution to the work reported, whether that is in the conception, study design, execution, acquisition of data, analysis and interpretation, or in all these areas; took part in drafting, revising or critically reviewing the article; gave final approval of the version to be published; have agreed on the journal to which the article has been submitted; and agree to be accountable for all aspects of the work.
DisclosureThe authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
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