Red-complex bacteria exhibit distinctly different interactions with human periodontal ligament stromal cells compared to Fusobacterium nucleatum

AbbreviationsCLSM

confocal laser scanning microscopy

DAPI

4′,6-diamidino-2-phenylindole

DMEM

Dulbecco's Modified Eagle's Medium

ELISA

enzyme-linked immunosorbent assay

F. nucleatum

Fn, Fusobacterium nucleatum

FA

Fastidious Anaerobe agar

GAPDH

glyceraldehyde 3-phosphate dehydrogenase

hPDL-MSCs

human periodontal ligament mesenchymal stem cells

MCP-1

monocyte chemoattractant protein-1

MOI

multiplicity of infection

MTT

3-(4,5-dimethylthiazol-2-yl)−2-diphenyltetrazolium bromide

P. gingivalis

Pg, Porphyromonas gingivalis

PBS

phosphate-buffered saline

Pen-Strep

penicillin-streptomycin

qRT-PCR

quantitative real-time polymerase chain reaction

RANKL

receptor activator of nuclear factor-B ligand

SEM

standard error of the mean

T. forsythia

Tf, Tannerella forsythia

TNF-α

tumor necrosis factor α

Keywords

Porphyromonas gingivalis

Tannerella forsythia

Human periodontal ligament mesenchymal stromal cells

Inflammatory mediators

Bacterial viability

© 2024 The Author(s). Published by Elsevier Ltd.

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