A sensitive sandwich-type electrochemical aptasensing platform based on Ti3C2Tx/MoS2/MWCNT@rGONR for simultaneous detection of kanamycin and chloramphenicol in food sample

The Ti3C2Tx/MoS2/MWCNT@rGONR nanocomposite was firstly prepared for building a sensitive electrochemical aptasening platform to simultaneous detect kanamycin (Kana) and chloramphenicol (Cap). Owning to the accordion-like structure, rich surface groups, and high charge mobility, the Ti3C2Tx/MoS2/MWCNT@rGONR provided a spacious covalent immobilization surface and a better electrochemical aptasensing platform. The aptamers of Kana and Cap used in sensor enhancing the selectivity. Furthermore, TiP loading more different metal ion were used as label to form a sandwich-type sensor together with Ti3C2Tx/MoS2/MWCNT@rGONR, which further improving the electrochemical sensitivity and obtaining high distinguishable signal readout. Under the optimized conditions, the sensor has good detection limits of Kana and Cap respectively at 0.135 nmol/L and 0.173 nmol/L in a same linearity concentration of 0.5~2500 nmol/L. Finally, it was successfully applied for detection in milk and fish meat and the results were compared with standard method HPLC, indicating a great potential for food safety monitor.

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