Available online 30 April 2024, 104108
Author links open overlay panel, Highlights•Highest survival rate of S.mutans CAD disinfected with Chlorhexidine
•Lowest survival rate of S.mutand CAD disinfected with PC@AgNPs-PDT
•Maximum outcomes of bond integrity was observed in group 4 CAD disinfected with PC@AgNPs-PDT
•CAD disinfected with Nd:YAG laser demonstrated the lowest bond scores
ABSTRACTAIMSTo assess the impact of various cavity disinfectants PC-PDT (Phycocyanin activated by Photodynamic therapy), PC@AgNPs-PDT (Phycocyanin and silver nanoparticles activated by PDT), and Nd: YAG laser on the survival rate of S.mutans and the bond integrity of composite restoration
METHODSSixty human mandibular molars that scored 4 and 5 based on ICDAS criteria were included. The infected dentin was removed while the CAD was preserved based on visual, tactile, and staining assessment. S.mutans were cultured on the CAD of twenty samples. All the specimens were indiscriminately distributed into four groups based on cavity disinfection (n=20 each includes n=5 each group incubated with S.mutans) Group 1: CHX, Group 2: Nd:YAG laser, Group 3: PC-PDT and Group 4: PC@AgNPs-PDT. S.mutans survival rate was assessed for each group(n=5). Forty samples underwent composite bonding for SBS and failure mode assessment using universal testing machine (UTM) and stereomicroscope. The calculations for the mean and standard deviation (SD) and their comparison among different groups were performed using a one-way analysis of variance (ANOVA) and the Tukey post hoc test (p ≤ 0.05)
RESULTSCAD surface treated disinfected with PC@AgNPs-PDT yielded the lowest survival rates (0.13 ± 0.05 CFU/ml) and highest SBS (17.23 ± 1.45 MPa). Group 1 (CHX) unveiled the highest survival rate of S.mutans (0.33 ± 0.12 CFU/ml). However, Group 2 (Nd:YAG Laser) (11.87 ± 0.67 MPa) presented the lowest SBS
CONCLUSIONThe combination of Phycocyanin loaded with silver nanoparticles and activated with Photodynamic therapy demonstrates the highest antimicrobial potential and bond strength of composite restorations.
KeywordsSilver Nanoparticles
Shear bond strength
Phycocyanin
Carious affected dentin
Photodynamic therapy
© 2024 Published by Elsevier B.V.
留言 (0)