Objectives To develop reference intervals for leukocyte lysosomal enzymology.

Design Standards-based establishment of reference intervals by analysis of 7851 for 17 assays of 15 lysosomal enzymes in leukocyte and plasma (January 1985 through September 2013).

Methods Reference intervals were calculated using MedCalc for Windows version 12.5 (MedCalc Software, Ostend, Belgium), according to the Clinical and Laboratory Standards Institute C-28A protocol (CSLI-C28A).

Results and Conclusion Evidence based reference intervals are presented in tabular form for seventeen assays of fifteen lysosomal enzymes.

The authors have declared no competing interest.

Work funded through employment with SA PAthology, a public laboratory.

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The details of the IRB/oversight body that provided approval or exemption for the research described are given below:

Data was accessed in the study under and institutional review board waiver for use of data under quality assurance purposes, in this case specifically for the development of reference intervals. The institutional review board is that of South Australia (SA) Pathology.

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