As a thriving group of two-dimensional (2D) nanomaterials, MXenes offer tremendous application and development prospects because of their excellent physical and chemical properties. Rhizosphere bacteria colonize the narrow ecosystem region surrounding plant roots and are critical in supporting the growth and ecological fitness of the host plant, while also exhibiting sensitivity to variations in the soil environment. However, through complex and unrecognized interactions, MXenes may have unexpected effects on the rhizosphere bacterial community; therefore, their potential risks must be assessed before exposure to the soil environment. In this study, we optimized the efficient one-step mechanical exfoliation (ECO-ME) method, enabling the successful multiple times preparation of MXene within one day. Subsequently, the influence of the prepared E-Ti3C2 on the rhizosphere bacterial communities of tomato plants was assessed by high-throughput sequencing. Surprisingly, no significant variations in the rhizosphere bacterial communities were observed even with directly applied 10 mL of 1200 µg mL-1 E-Ti3C2 dispersion through the root irrigation strategy. Furthermore, the triggered slight fluctuations reverted to the initial state after 6 days, confirming the negligible effect of introducing E-Ti3C2 into the soil environment. This study offers a rapid and economical method for MXene preparation and provides a prospective investigation of the soil environmental risk for practical MXene applications.

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