Herein a magnetic beads-based chemiluminescence assay was reported to detect Type IV collagen (col-Ⅳ) in serum samples. Magnetic beads (MBs) exhibit the biocompatibility properties. Taking the advantages of this property, it was conjugated with the col-Ⅳ antibody. For the determination of col-Ⅳ, the interaction of the col-Ⅳ sample, anti-(col-Ⅳ)-alkaline phosphatase (anti-(col-Ⅳ)-ALP) and anti-col-IV-magnetic beads (anti-(col-Ⅳ)-MBs) was performed to generate the chemiluminescence intensity. Under the optimized conditions, the developed method displayed the good linearity in the concentration range of 20-2000 ng/mL with the limit of 0.79 ng/mL. The repeatability coefficient of variation (CV) for col-Ⅳ detection ranged from 3.16% to 7.50%. The col-Ⅳ level in the sample collected from the hospital was assessed by the chemiluminescence assay. The satisfactory recoveries was ranged from 93.30% to 100.14%. In conclusion, the magnetic beads-based chemiluminescence assay may be used as a routine and efficient tool to detect Type IV collagen in the clinical diagnosis.

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