Abstract With the increasing incidence of colorectal cancer, understanding different subgroups, such as the microsatellite stable (MSS) population, is crucial. Our study aimed to explore the unique characteristics and underlying genetic and epigenetic features of the microsatellite stable (MSS) population within colorectal cancer. Using 325 samples, we compared MSS with other microsatellite statuses on more than 50 clinical indicators. A significantly different overall gene mutation rate was observed in the MSS group compared to the other groups, especially in P53 mutations. No notable changes were found in epigenetics. The results suggest that MSS colorectal cancer cases are more likely to have gene mutations than other subgroups. We also found that the N stage was lower in the MSI-H group. These findings provide crucial insights that can guide future drug development and treatment plans for the MSS population. Keywords: MSI, P53, Mutation, Epigenetics, Colorectal Cancer

The authors have declared no competing interest.

This study did not receive any funding

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Ethics Committee of Xiangya Hospital, Central South University gave ethical approval for this work.

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All data produced in the present study are available upon reasonable request to the authors