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The following concerns were raised about Figs 3 and 5 of this article [1]:
In Fig 5Ac, lanes 2 and 3 appear similar. When levels are adjusted to visualize background, there is no visible evidence that Figs 3B, 3H and 5Bd FITC panels show the same fields of view as the corresponding white light microscopy (Fig 3) and Hoechst/Cy3/Merge (Fig 5Bd) panels.Corresponding author YH stated that the original data underlying the results reported in this article are no longer available, and so the above issues cannot be resolved.
In addition, the study reports the use of “human normal hepatocyte L-02” cells in flow cytometry experiments shown in Fig 6B. Following the publication of this article [1], the L-02 cell line was identified as contaminated and shown to be a HeLa derivative instead [2]. Therefore, the results statements in [1] about effects of rCsseverin on human normal hepatocyte cells are not supported, although this may not critically impact the PLC cell results reported in Fig 6A.
The PLOS Neglected Tropical Diseases Editors issue this Expression of Concern to inform readers of these unresolved issues. Readers should interpret the results of the affected figures with caution.
1. Chen X, Li S, He L, Wang X, Liang P, Chen W, et al. (2013) Molecular Characterization of Severin from Clonorchis sinensis Excretory/Secretory Products and Its Potential Anti-apoptotic Role in Hepatocarcinoma PLC Cells. PLoS Negl Trop Dis 7(12): e2606. https://doi.org/10.1371/journal.pntd.0002606 2. Ye F, Chen C, Qin J, Liu J, Zheng C (2015) Genetic profiling reveals an alarming rate of cross-contamination among human cell lines used in China. FASEB J 29(10):4269–72. pmid:26116706Citation: The PLOS Neglected Tropical Diseases Editors (2024) Expression of Concern: Molecular Characterization of Severin from Clonorchis sinensis Excretory/Secretory Products and Its Potential Anti-apoptotic Role in Hepatocarcinoma PLC Cells. PLoS Negl Trop Dis 18(3): e0012043. https://doi.org/10.1371/journal.pntd.0012043
Published: March 19, 2024
Copyright: © 2024 The PLOS Neglected Tropical Diseases Editors. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
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