The integration of artificial intelligence (AI) in healthcare has emerged as a transformative force, promising to enhance medical diagnosis, treatment, and overall healthcare delivery. Hence, this study investigates the university students perceptions toward using AI in healthcare. A cross-sectional survey was conducted at two major universities using a paper-based questionnaire from September 2023 to November 2023. Participants’ views regarding using artificial intelligence in healthcare were investigated using 25 items distributed across five domains. The Mann-Whitney U test was applied for the comparison of variables. The response rate for the survey was 75%, with a sample size of 279. More than half of the participants (52%, n = 145) expressed their belief in AI’s potential to reduce treatment errors in the future. However, about (61.6%, n = 172) of participants fear the influence of AI that could prevent doctors from learning to make correct patient care judgments, and it was widely agreed (69%) that doctors should ultimately maintain final control over patient care. Participants with experience with AI, such as engaging with AI chatbots, significantly reported higher scores in both the “Benefits and Positivity Toward AI in Healthcare” and “Concerns and Fears” domains (p = 0.024) and (p = 0.026), respectively. The identified cautious optimism, concerns, and fears highlight the delicate balance required for successful AI integration. The findings emphasize the importance of addressing specific concerns, promoting positive experiences with AI, and establishing transparent communication channels. Insights from such research can guide the development of ethical frameworks, policies, and targeted interventions, fostering a harmonious integration of AI into the healthcare landscape in developing countries.

The authors have declared no competing interest.

This study did not receive any funding.

I confirm all relevant ethical guidelines have been followed, and any necessary IRB and/or ethics committee approvals have been obtained.

Yes

The details of the IRB/oversight body that provided approval or exemption for the research described are given below:

Consent and ethical approval Verbal consent was obtained from all participants included in the study. They were informed about the proposal of the study, their rights as participants, and the confidentiality of their data. Their voluntary participation was acknowledged, and they were assured that their identities would remain anonymous throughout the study. The study design and procedure were approved by the Institutional Review Board at Aden University, Aden, Yemen, in compliance with the International Conference of Harmonization (ICH) Research Code (REC-164-2023).

I confirm that all necessary patient/participant consent has been obtained and the appropriate institutional forms have been archived, and that any patient/participant/sample identifiers included were not known to anyone (e.g., hospital staff, patients or participants themselves) outside the research group so cannot be used to identify individuals.

Yes

I understand that all clinical trials and any other prospective interventional studies must be registered with an ICMJE-approved registry, such as ClinicalTrials.gov. I confirm that any such study reported in the manuscript has been registered and the trial registration ID is provided (note: if posting a prospective study registered retrospectively, please provide a statement in the trial ID field explaining why the study was not registered in advance).

Yes

I have followed all appropriate research reporting guidelines, such as any relevant EQUATOR Network research reporting checklist(s) and other pertinent material, if applicable.

Yes

All data produced in the present study are available upon reasonable request to the authors.