Int J Angiol
DOI: 10.1055/s-0044-1779663
Yu Qiu
1
Department of Vascular and Endovascular Surgery, Second Affiliated Hospital of Naval Medical University, Shanghai, China
,
Meiying Yang
2
Department of Trauma Orthopedics, Zibo Central Hospital, Zibo, China
,
Xinting Che
3
Department of Vascular and Endovascular Surgery, School of Medicine, Xiamen University, Xiamen, China
,
Xinming Yu
4
Department of Vascular Surgery, Zibo Central Hospital, Zibo, China
,
Kangkang Zhi
1
Department of Vascular and Endovascular Surgery, Second Affiliated Hospital of Naval Medical University, Shanghai, China
› Author Affiliations
Funding This study was funded by Shanghai 2020 “Science and Technology Innovation Action Plan” Biomedical Science and Technology Support Special Project (20S31902000) and the National Natural Science Foundation of China (81971710).
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Abstract
Deep vein thrombosis (DVT) is a common postoperative complication of orthopaedic surgery with a complex pathogenesis mechanism. The effect of the miR-2467-3p/acting-binding LIM protein 1 (ABLIM1) axis on thrombus formation and human vascular endothelial cells (HUVECs) progression was evaluated aiming to identify a novel potential biomarker of DVT. DVT rat models were established by inferior vena cava stenosis. The expression of the miR-2467-3p/ABLIM1 axis was analyzed by PCR. HUVECs were induced with oxidative low-density lipoprotein (ox-LDL). Cell growth and motility were assessed by cell counting kit 8 (CCK8) and Transwell assay. The inflammation and oxidative stress were estimated by proinflammatory cytokines and generation of MDA and reactive oxygen species (ROS). ABLIM1 was downregulated in DVT rats. Overexpressing ABLIM1 could suppress the formation of thrombosis and alleviate inflammation and oxidative stress. In HUVECs, ox-LDL induced significantly increased miR-2467-3p and decreased ABLIM1, and miR-2467-3p could negatively regulate ABLIM1. The knockdown of miR-2467-3p could alleviate the inhibited cell growth and motility by ox-LDL, and the inflammation and oxidative stress were also attenuated. While silencing could reverse the effect of miR-2467-3p on ox-LDL-induced HUVECs. The miR-2467-3p/ABLIM1 axis regulates the occurrence and development of DVT through modulating HUVECs inflammation and oxidative stress.
Keywords
microRNA -
HUVECs injury -
ceRNA -
cell proliferation -
cell migration
Authors' Contributions
Y.Q. and X.M.Y designed the research study. M.Y.Y and X.T.C performed the research. Y.Q., X.M.Y, and K.K.Z analyzed the data and wrote the manuscript. All authors contributed to editorial changes in the manuscript. All authors read and approved the final manuscript.
Ethical Approval
The study protocol was approved by The Ethics Committee of Zibo Central Hospital and followed the principles outlined in the Declaration of Helsinki.
Patient Consent
Iinformed consent has been obtained from the participants involved.
Publication History
Article published online:
21 February 2024
© 2024. International College of Angiology. This article is published by Thieme.
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