Eur Rev Med Pharmacol Sci 2023; 27 (10): 4369-4377

DOI: 10.26355/eurrev_202305_32441

Z.-R. Ye, Y.-J. Lin, Y.-N. Cai, N.-J. Hong, D.-L. Kang, M. Li, Z.-X. Zeng

Department of General Practice, Quanzhou First Hospital, Quanzhou, Fujian, China. zxiongzeng@163.com

OBJECTIVE: Atherosclerosis (As) is an inflammatory disease, and 2,3,4′,5-tetrahydroxystilbene-2-O-β-d-glucoside (TSG) has been shown to suppress inflammation. However, it is still unclear if TSG alleviates As by inhibiting inflammation.

MATERIALS AND METHODS: Quantitative real-time polymerase chain reaction (qRT-PCR) was performed to assess the mRNA levels of tumor necrosis factor (TNF) receptor-associated factor 6 (TRAF6), TNF-α and interleukin-6 (IL-6) in lipoprotein E knockout (ApoE -/-) mice with As. Hematoxylin-eosin (H&E) staining was performed to examine the atherosclerotic plaques in the aortic sinus. QRT-PCR and western blotting were used to measure the expression levels of TRAF6, TNF-α, and IL-6 in human umbilical vein endothelial cells (HUVECs), and enzyme-linked immunosorbent assays (ELISAs) were performed to monitor the levels of TNF-α and IL-6 in serum and cell culture medium.

RESULTS: TSG inhibited subendothelial plaques formation in the aortic sinus and inhibited the levels of total cholesterol (TCHO), low-density lipoprotein (LDL), TRAF6, TNF-α and IL-6 in AS mice in a dose-dependent manner. Moreover, TSG attenuated the oxidatively modified LDL (ox-LDL)-induced increases in TRAF6, TNF-α and IL-6 expression, whereas TRAF6 overexpression reversed the TSG-induced decreases in TRAF6, TNF-α, and IL-6 expression in HUVECs.

CONCLUSIONS: TSG attenuates atherosclerotic progression by inhibiting inflammation via the downregulation of TRAF6 in ApoE-/- mice and HUVECs.

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Z.-R. Ye, Y.-J. Lin, Y.-N. Cai, N.-J. Hong, D.-L. Kang, M. Li, Z.-X. Zeng
2,3,4’,5-Tetrahydroxystilbene-2-O-β-d-glucoside attenuates atherosclerotic progression by inhibiting inflammation via downregulation of TNF receptor-associated factor 6 expression

Eur Rev Med Pharmacol Sci
Year: 2023
Vol. 27 - N. 10
Pages: 4369-4377
DOI: 10.26355/eurrev_202305_32441